Rapid propagation method for dendrobii officmalis caulis

A rapid technology of Dendrobium candidum, applied in the field of rapid propagation of Dendrobium candidum, can solve problems such as low efficiency of propagation technology, and achieve the effects of shortening the seedling raising period, making the plants robust, and reducing the pollution rate.

Inactive Publication Date: 2015-11-18
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved in the present invention is to provide a rapid propagation method of Dendrobium officinale, to solve the problem of low efficiency of propagation technology existing in the prior art

Method used

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  • Rapid propagation method for dendrobii officmalis caulis
  • Rapid propagation method for dendrobii officmalis caulis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A kind of rapid propagation method of dendrobium candidum, concrete steps are as follows:

[0033] 1. Rinse the 20-week-old uncracked mature capsules collected in the field under tap water, and after natural air-drying, soak them in 75% ethanol aqueous solution for 45 seconds in an ultra-clean workbench, take them out and rinse them with sterilized distilled water for 3 times then soak and sterilize the capsules in 0.1% mercuric chloride aqueous solution for 10 minutes, rinse 6 times with sterilized distilled water after taking out, and stir from time to time; finally dry the moisture on the surface of the capsules with sterilized filter paper, and use Remove the seeds from the middle of the capsule with a sterilized scalpel.

[0034] 2. Sow the taken Dendrobium officinale seeds evenly on the seed germination medium. The medium formula is: MS+0.5mg / LNAA+1.0mg / L6-BA+50g / L potatoes+25g / L sucrose+6.0g / L Agar powder, pH5.8. At 22°C, culture in dark for 7 days, and change ...

Embodiment 2

[0039] A kind of rapid propagation method of dendrobium candidum, concrete steps are as follows:

[0040] 1. Rinse the 20-week-old uncracked mature capsules collected in the field under tap water, and after natural air-drying, soak them in 75% ethanol aqueous solution for 45 seconds in an ultra-clean workbench, take them out and rinse them with sterilized distilled water for 3 times then soak and sterilize the capsules in 0.1% mercuric chloride aqueous solution for 10 minutes, rinse 6 times with sterilized distilled water after taking out, and stir from time to time; Remove the seeds from the middle of the capsule with a sterilized scalpel.

[0041] 2. Sow the taken Dendrobium officinale seeds evenly on the seed germination medium. The medium formula is: MS+0.5mg / LNAA+0.5mg / L6-BA+50g / L potato+25g / L sucrose+6.0g / L Agar powder, pH5.8. At 22°C, culture in dark for 7 days, and change the culture conditions after 7 days, and cultivate for 60 days at a temperature of 25°C, a light...

Embodiment 3

[0046] A kind of rapid propagation method of dendrobium candidum, concrete steps are as follows:

[0047] 1. Rinse the 20-week-old uncracked mature capsules collected in the field under tap water, and after natural air-drying, soak them in 75% ethanol aqueous solution for 45 seconds in an ultra-clean workbench, take them out and rinse them with sterilized distilled water for 3 times then soak and sterilize the capsules in 0.1% mercuric chloride aqueous solution for 10 minutes, rinse 6 times with sterilized distilled water after taking out, and stir from time to time; finally dry the moisture on the surface of the capsules with sterilized filter paper, and use Remove the seeds from the middle of the capsule with a sterilized scalpel.

[0048] 2. Sow the taken Dendrobium officinale seeds evenly on the seed germination medium. The medium formula is: MS+0.5mg / LNAA+1.5mg / L6-BA+50g / L potato+25g / L sucrose+6.0g / L Agar powder, pH5.8. At 20°C, culture in dark for 7 days, change the cu...

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Abstract

The invention discloses a rapid propagation method for dendrobii officmalis caulis. The rapid propagation method comprises the following steps: sterilizing the surfaces of seed capsules, sowing the sterilized seeds in a germination culture medium, grafting seedlings germinated by seeds to a protocorm differential medium, transferring differentiated seedlings to a strong seedling rooting medium to finally obtain the tissue culture seedlings of dendrobii officmalis caulis. Compared with the prior art, the method has the advantages that uncracked capsules of dendrobii officmalis caulis are adopted so as to effectively prevent endophytes of dendrobii officmalis caulis from bringing troubles to tissue culture and lower pollution rate; a tri-generation culturing method including seed germination, subculture and strong seedling rooting is adopted, so that the seedling period is greatly shortened and the production cost is lowered; and the cultured tissue culture seedlings are robust, and have developed roots and high survival rate.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a rapid propagation method of dendrobium officinale. Background technique [0002] Dendrobium officinale, also known as black knot grass, is a perennial herb of the genus Dendrobium orchidaceae. It grows in mountains at an altitude of 100 to 3,000 meters. It likes a warm and humid environment with half shade and half sun, and is not cold-tolerant. Mainly distributed in Anhui, Zhejiang, Yunnan, Fujian and other places. Dendrobium officinale is used as medicine with its fresh or dried stems. It is sweet in taste and slightly cold in nature. The main medicinal ingredients are dendrobium polysaccharides, alkaloids and amino acids. "Included. The seeds of Dendrobium officinale are as small as dust and have no endosperm. It is extremely difficult to germinate under natural conditions. Coupled with artificial over-harvesting, its resources are on the verge of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 席刚俊徐超史俊汪善锋杨鹤同郑凯贾君赵桂华
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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