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An inducer for inducing mesenchymal stem cells into neurons and a complete culture medium for inducing differentiation

A complete medium and induction differentiation technology, applied in the field of stem cell induction and differentiation, can solve the problems of high cell death rate, low induction efficiency, long induction time, etc., and achieve the effect of good cell activity, high induction efficiency, and short induction time.

Active Publication Date: 2017-12-29
QINGDAO RESTORE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although the traditional chemical inducer method can efficiently and quickly differentiate mesenchymal stem cells into nerve cells, the cell death rate is high (chemical inducers have certain toxicity, BHA, DMSO, etc. risk of tumorigenicity); although the growth factor induction method has a high cell survival rate after induction, the induction time is long and the induction efficiency is low
Gene transfection induces potential risk of cancer due to genetic alterations

Method used

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  • An inducer for inducing mesenchymal stem cells into neurons and a complete culture medium for inducing differentiation
  • An inducer for inducing mesenchymal stem cells into neurons and a complete culture medium for inducing differentiation
  • An inducer for inducing mesenchymal stem cells into neurons and a complete culture medium for inducing differentiation

Examples

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Effect test

Embodiment 1

[0020] Example 1 An inducer that induces mesenchymal stem cells into neuroblasts, including resveratrol, icariin, hydrocortisone, vascular endothelial growth factor (VEGF), insulin-like growth factor-I (IGF -I), erythropoietin (EPO).

Embodiment 2

[0021] Example 2 In the complete culture medium for inducing mesenchymal stem cells into neurons in this example, the following raw materials were dissolved according to their respective characteristics, and the serum-free complete medium for human mesenchymal stem cells was used as the substrate (LONZA, catalog number 00190632 ), so that the content of each component was adjusted to 1000ml as follows: resveratrol (Sigma, R5010-100MG) 36g, icariin (Shanghai Microcrystalline Bio, 489-32-7, 20mg) 7μmol, hydrocortisone Pine (Sigma, H3160) 6 nmol, VEGF (PeproTech, 96-100-20-2) 10 μg, IGF-I (PeproTech, 96-100-11-20) 6 μg, EPO (PeproTech, CYT-201) 3 μg.

Embodiment 3

[0022] Example 3 In the complete medium for inducing differentiation of mesenchymal stem cells into neurons in this example, the following raw materials were dissolved according to their respective characteristics, and human mesenchymal stem cell serum-free medium was used as the substrate (LONZA, catalog number 00190632) , so that the content of each component was adjusted to 1000ml as follows: resveratrol (Sigma, R5010-100MG) 40g, icariin (Shanghai microcrystalline biology, 489-32-7, 20mg) 8μmol, hydrocortisone (Sigma, H3160) 8nmol, VEGF (PeproTech, 96-100-20-2) 15μg, IGF-I (PeproTech, 96-100-11-20) 7μg, EPO (PeproTech, CYT-201) 4μg.

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Abstract

The invention belongs to the technical field of stem cell induction differentiation, particularly relates to an inducer and an induction medium, and discloses an inducer and induced differentiation complete medium for inducing mesenchymal stem cells into nerve cells. The inducer for inducing the mesenchymal stem cells into the nerve cells comprises resveratrol, icariin, hydrocortisone, VEGF, IGF-I and EPO. Every one thousand milliliters of the induced differentiation complete medium for inducing the mesenchymal stem cells into nerve cells comprises 30-50 micromoles of resveratrol, 5-10 micromoles of icariin, 5-10 micromoles of hydrocortisone, 10-20 micrograms of VEGF, 5-10 micrograms of IGF-I, 2-5 micrograms of EPO and the balance mesenchymal stem cell serum-free complete media. According to the induced differentiation complete medium for inducing the mesenchymal stem cells into the nerve cells, the traditional Chinese medicine ingredients of resveratrol and icariin are combined with hydrocortisone and the growth factors of VEGF, IGF-I and EPO to synergistically induce the mesenchymal stem cells to be directionally differentiated into the nerve cells, and the selected induction components are all free of poison, high in induction efficiency, short in induction time, good in activity of the nerve cells obtained through induction, free of rejection after cell transplantation, free of ethical issues and high in safety.

Description

technical field [0001] The invention belongs to the technical field of induction and differentiation of stem cells, and in particular relates to an inducer and a culture medium for inducing differentiation. Background technique [0002] Since the first report of the application of mesenchymal stem cells (MSCs) in clinical trials in 1995, the cultured MSCs have been widely used in clinical trials, including graft-versus-host disease (GVHD), congestive heart failure, acute myocardial infarction , type 2 diabetes, spinal cord injury, cartilage and bone damage, Crohn's disease, and more. On the other hand, with the continuous discovery of new sources of mesenchymal stem cells, such as fat, amniotic fluid, placenta, umbilical cord, umbilical cord blood, etc., the source of mesenchymal stem cells has been expanded, and various therapeutic methods based on mesenchymal stem cells have been expanded. More and better sources of seed cells are provided. The door to the marketization ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/079C12N5/0775
Inventor 张炳强
Owner QINGDAO RESTORE BIOTECHNOLOGY CO LTD
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