Crocus sativus L. tissue cultured corm strengthening and rooting medium and tissue culture method

Abstract

The invention discloses a Crocus sativus L. tissue cultured corm strengthening and rooting medium and a tissue culture method. The provided medium employs an LS medium as a basic medium, 1L of the medium comprises 6-8g of carrageenan, 40-60 parts of white sugar, 0.1-0.5 part of active carbon, 20-40 parts of banana flesh, 10-30g of mashed potato, 0.1-1mg of naphthylacetic acid, 1-5mg of indolebutyric acid and 1-5mg of paclobutrazol, and the pH value is 5.8-6.0. The provided medium can promote strengthening and rooting of Crocus sativus L. tissue cultured corm, root warping is not easy, and the transplanting survival rate is raised. The tissue culture method comprises the following steps: firstly, Crocus sativus L. multiple shoots are taken and inoculated into an induction medium and a corm is obtained after induction culture; secondly, the corm is inoculated in the provided medium, and the corm is cultured until the diameter is not less than 10mm. The diameter of the Crocus sativus L. tissue cultured corm cultured through the method is 2.2 times of a diameter of the Crocus sativus L. corm cultured without the strengthening medium, the tissue cultured corm quality is raised, and the field growth cycle of corms is shortened.

Description

technical field

[0001] The invention belongs to the field of plant tissue culture, and in particular relates to a saffron tissue culture corm rooting medium and a tissue culture method. Background technique

[0002] Saffron (Crocussativus L.), also known as saffron and crocus, is a perennial plant of the genus Crocus in the family Iridaceae, and is also a common spice. It is mainly distributed in Europe, the Mediterranean and Central Asia, and is cultivated in Beijing, Shandong, Zhejiang and Sichuan in China. Saffron is a kind of precious Chinese medicinal material. It has three red stigmas on the upper part of the pistil. It has various effects such as lowering blood fat, and also has good immunity-enhancing and anti-tumor activities.

[0003] In my country, saffron mostly adopts the "two-stage" cultivation method, that is, the bulbs are dug up from the ground in May of that year, and the indoor cultivation and flower picking are carried out, and the flower-picked bulbs a...

Examples

Embodiment 1

[0034] (1) with culture medium:

[0035] Each liter of bulblet induction medium consists of the following components: LS+white sugar 50g+NAA 0.5mg+agar powder 7g, pH=5.8.

[0036] The rooting medium for strong balls per liter consists of the following components: LS + 50g of sugar + 30g of banana meat + 20g of mashed potato + 2mg of paclobutrazol + 3mg of IBA + 0.5mg of NAA + 7g of carrageenan + 0.2g of activated carbon, pH=5.8.

[0037] (2) Select the clustered buds of saffron that grow robustly, cut them into individual buds, inoculate them into the bulblet induction medium, culture at 20±2°C, and cultivate under light conditions (12h / d, light intensity 1500lux) for 50 days Small bulbs with a diameter of about 5mm were induced from left to right.

[0038] (3) Inoculate the induced bulblets into the strong bulb rooting medium, cultivate them at a temperature of 20±2°C, and cultivate them under light conditions (12h / d, light intensity 1500lux), and the diameter of the bulblet...

Embodiment 2

[0040] The small bulb induction medium adopts the medium formula in Example 1, and the strong bulb rooting medium formula is as follows: in 1 L, LS+white sugar 40g+banana meat 30g+mashed potato 20g+paclobutrazol 2mg+IBA1mg+NAA1mg+carrageenan 7g+activated carbon 0.2g, pH = 5.8.

[0041] The bulblet induction and culture conditions are the same as in Example 1.

Embodiment 3

[0043] The small bulb induction medium adopts the medium formula in Example 1, and the strong bulb rooting medium formula is as follows: in 1 L, LS+white sugar 60g+banana meat 30g+mashed potato 20g+paclobutrazol 3mg+IBA2mg+NAA0.8mg+carrageenan 7g+activated carbon 0.1g , pH=5.8.

[0044] The bulblet induction and culture conditions are the same as in Example 1.

technical field

[0001] The invention belongs to the field of plant tissue culture, and in particular relates to a saffron tissue culture corm rooting medium and a tissue culture method. Background technique

[0002] Saffron (Crocussativus L.), also known as saffron and crocus, is a perennial plant of the genus Crocus in the family Iridaceae, and is also a common spice. It is mainly distributed in Europe, the Mediterranean and Central Asia, and is cultivated in Beijing, Shandong, Zhejiang and Sichuan in China. Saffron is a kind of precious Chinese medicinal material. It has three red stigmas on the upper part of the pistil. It has various effects such as lowering blood fat, and also has good immunity-enhancing and anti-tumor activities.

[0003] In my country, saffron mostly adopts the "two-stage" cultivation method, that is, the bulbs are dug up from the ground in May of that year, and the indoor cultivation and flower picking are carried out, and the flower-picked bulbs a...

Examples

Embodiment 1

[0034] (1) with culture medium:

[0035] Each liter of bulblet induction medium consists of the following components: LS+white sugar 50g+NAA 0.5mg+agar powder 7g, pH=5.8.

[0036] The rooting medium for strong balls per liter consists of the following components: LS + 50g of sugar + 30g of banana meat + 20g of mashed potato + 2mg of paclobutrazol + 3mg of IBA + 0.5mg of NAA + 7g of carrageenan + 0.2g of activated carbon, pH=5.8.

[0037] (2) Select the clustered buds of saffron that grow robustly, cut them into individual buds, inoculate them into the bulblet induction medium, culture at 20±2°C, and cultivate under light conditions (12h / d, light intensity 1500lux) for 50 days Small bulbs with a diameter of about 5mm were induced from left to right.

[0038] (3) Inoculate the induced bulblets into the strong bulb rooting medium, cultivate them at a temperature of 20±2°C, and cultivate them under light conditions (12h / d, light intensity 1500lux), and the diameter of the bulblet...

Embodiment 2

[0040] The small bulb induction medium adopts the medium formula in Example 1, and the strong bulb rooting medium formula is as follows: in 1 L, LS+white sugar 40g+banana meat 30g+mashed potato 20g+paclobutrazol 2mg+IBA1mg+NAA1mg+carrageenan 7g+activated carbon 0.2g, pH = 5.8.

[0041] The bulblet induction and culture conditions are the same as in Example 1.

Embodiment 3

[0043] The small bulb induction medium adopts the medium formula in Example 1, and the strong bulb rooting medium formula is as follows: in 1 L, LS+white sugar 60g+banana meat 30g+mashed potato 20g+paclobutrazol 3mg+IBA2mg+NAA0.8mg+carrageenan 7g+activated carbon 0.1g , pH=5.8.

[0044] The bulblet induction and culture conditions are the same as in Example 1.