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Method for extracting hirudin by electrically stimulating living leeches

A hirudin and electrical stimulation technology, applied in the preparation method of peptides, from leech inhibitors, chemical instruments and methods, etc., can solve problems such as unsuccessful implementation, overcome seasonal obstacles, improve product purity, and maintain repeatability The effect of using

Inactive Publication Date: 2015-12-16
南宁市和兰记生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the extraction of hirudin, the live leeches are directly stimulated by weak direct current electricity, so that their salivary glands are stressed to secrete hirudin-rich saliva, so as to realize the batch extraction of high-purity hirudin and maintain the living traits of leeches by controlling the current The method simplifies the production process of hirudin, improves the product purity, keeps the repeated utilization of wild leech resources, reduces the production cost, and overcomes the method of the seasonal obstacle problem of production, so far there is no report of successful realization

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] At a room temperature of 1-30 degrees, first take the living leeches from the breeding tank to the storage tank, rinse with deionized water for 20 minutes to remove the impurities on the surface of the living leeches, filter out the water, and then immerse in physiological saline to rinse for 20 seconds to cover the surface of the leeches Electrolyte, filter out the salt water, put it in a rectangular stainless steel filter tank with a 200-mesh filter at the bottom (the filter tank is insulated from the ground) and spread it flat. Under the filter are disinfected and clean utensils of the same area. The two ends of the filter tank are respectively connected to the two poles of the direct current. Adjust to the minimum output current, combine the power switch, adjust the current to 1-20mA, after 5-30 minutes, disconnect the power, collect the liquid dripping from the filter tank in the vessel, and then pour the leeches from the filter tank back to the storage tank. Pour ba...

Embodiment 2

[0018] At a room temperature of 1-30 degrees, first take the living leeches from the breeding tank to the storage tank, rinse with deionized water for 2 minutes to remove the impurities on the surface of the living leeches, filter out the water, and then immerse in physiological saline to rinse for 20 seconds, and put in the bottom stainless steel The basket of the net is immersed in a salt water tank with a concentration of 0.5 to 1%. Direct current is applied to the salt water tank to make the salt water in the tank live. Adjust to the minimum output current. Combine the power switch to adjust the current to 1~ 20mA, 5-30 minutes later, remove, and then pour the leeches from the basket of the rust-steel mesh back to the storage tank, then back to the breeding tank, and repeat the immersion of the other basket of the rust-steel mesh with the newly installed living leeches into the salt water tank , To achieve continuous production of hirudin extracted from living leeches.

[001...

Embodiment 3

[0021] At a room temperature of 1-30 degrees, first take the living leeches from the breeding tank to the storage tank, rinse with deionized water for 2 minutes to remove the impurities on the surface of the living leeches, filter out the water, and put them in a 50-200 liter plastic bucket, and then pour Fill in the salt water with a concentration of 0.5 to 1%, the volume of the salt water is 1:0.5 to 1.5 between the volume of the leeches and the volume of the salt water. The metal electrode is placed at the bottom of the plastic bucket, and the metal electrode is placed on the top of the plastic bucket to the liquid surface. Adjust the two poles to the minimum output current, merge the power switch, slowly adjust the current to 1-20mA, after 5-30 minutes, disconnect the power, pour the leeches from the plastic bucket back to the storage tank, and then back to the breeding tank. Collect the liquid in the plastic bucket. Most of this liquid is a mixture of hirudin active ingredi...

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PUM

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Abstract

The invention discloses a method for extracting hirudin by electrically stimulating living leeches. The method includes four steps of extraction of secretion, preparation of protectant, processing of secretion and drying of secretion. The product dry powder contains the main effectively active ingredient, namely hirudin, extracted from the living leeches, which can be used for preventing and treating vein thrombosis, disseminated intravascular coagulation, brain coagulation, thrombophlebitis, coronary thrombosis and the like. Compared with the prior art, the method has the advantages that hirudin production process is simplified while living leeches are maintained for extracting high-purity hirudin multiple times.

Description

Technical field [0001] The invention relates to a body leech that is activated by weak direct current stabs to make the head glands of the leech release gland fluid, and at the same time, by controlling the intensity of the current, it is ensured that the leech remains in a living state, so as to realize multiple removal from the living body under the condition of limited living resources of the leech A method of obtaining high-purity hirudin from the gland fluid of leeches. Background technique [0002] Hirudin is named after it was first isolated from leeches. Natural hirudin is composed of 65 amino acid residues and has a molecular weight of 7000 Daltons. It is a water-soluble animal protein peptide. Hirudin has the function of inactivating thrombin. The functional mechanism is that it can irreversibly bind to the catalytic center of thrombin and the fibrinogen binding center, so that thrombin can not achieve the coagulation function. Hirudin is currently the most potent thr...

Claims

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Application Information

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IPC IPC(8): C07K14/815C07K1/14
CPCC07K14/815
Inventor 苏国强刁建中
Owner 南宁市和兰记生物科技有限公司
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