A method for grading humic acid macroporous resin in algae
A classification method, humic acid technology, applied in the direction of organic chemistry, etc.
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Embodiment 1
[0059] Embodiment 1: Fractional extraction of humic acid in brown algae East China Sea sample:
[0060] In April 2014, samples of brown algae from the East China Sea were collected;
[0061] XAD-8 resin column; Sigma company;
[0062] Sodium hydroxide: analytically pure; hydrochloric acid: analytically pure; sodium pyrophosphate: analytically pure;
[0063] The specific operations of this embodiment are as follows:
[0064] Step a, algae body pretreatment: take the air-dried algae body, remove impurities, dry at room temperature, grind and sieve to obtain 5 kg of algae body powder;
[0065] Step b, algae Soxhlet extraction:
[0066] Put 5 kg of algae powder in a Soxhlet extractor, and use ether as the extraction solution for Soxhlet extraction. During the extraction process, measure the absorbance of the extraction solution under ultraviolet-visible light with a wavelength of 220nm every 5 hours. When the absorbance is greater than or When it is equal to 0.01, the process ...
Embodiment 2
[0110] Embodiment 2: This embodiment discloses a method for grading humic acid macroporous resins in algal bodies, and the method for grading and extracting comprises the following steps:
[0111] Step a, algae body pretreatment: take the air-dried algae body, remove the sundries, in 30-50 o Dry at C, and sieve after grinding to obtain the algae body powder;
[0112] Step b, Soxhlet extraction of algae body: place the algae body powder in a Soxhlet extractor, and use ether, acetone, 95% ethanol, and dioxane as the extracting solution to carry out extraction; during the extraction process, when When one of the extraction solutions is used for extraction, the absorbance of the extraction solution is measured under ultraviolet-visible light with a wavelength of 220nm every 5h. When the absorbance of the extraction solution is greater than or equal to 0.01, the extraction is continued. Process; when the measured absorbance is less than 0.01, replace the next extraction solution f...
Embodiment 3
[0131] Embodiment 3: The difference between this embodiment and Example 2 is that in step h in this embodiment, when washing the XAD-8 resin column with a sodium pyrophosphate buffer solution of a certain pH value, every 2- 50 ml of effluent is measured with specific wavelength UV / Visible wavelength. The UV / Visible absorbance of the effluent increases first and then decreases. When the UV / Visible absorbance of the effluent is less than 0.5% of the maximum absorbance, the pH value is stopped. Rinse with sodium pyrophosphate buffer.
[0132] In step i of this embodiment, after the dialysis system of each dialysis bag was stirred for 12 hours, the content of total phosphorus and the content of chloride ions in deionized water in step i were respectively determined by ammonium molybdate spectrophotometry and silver nitrate method, if the total phosphorus content > 0.01 mg / L or silver chloride exists, replace the ultrapure water in the dialysis system several times, add deionized w...
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