Full-scale range C-reactive protein latex-enhanced immunoturbidimetry detection kit

A detection kit and reactive protein technology, applied in the field of medical immunology, can solve the problems of inability to diagnose infectious diseases or observe curative effect, cannot be used as risk indicators of cardiovascular diseases, and cannot accurately measure CRP content, etc., so as to broaden the detection range , Reagent stability, high accuracy effect

Inactive Publication Date: 2015-12-16
NANJING WENZHI BIOTECH
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Problems solved by technology

The routine clinical method for measuring CRP is the immunoturbidimetric method, and the measuring range is generally 3-200 mg/L, but the sensitivity is relatively low, and the CRP content below 3 mg/L cannot be accurately measured. Therefore, it cannot be used as a risk indicator for cardiovascular diseases. Indicators to predict the risk of cardio

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  • Full-scale range C-reactive protein latex-enhanced immunoturbidimetry detection kit
  • Full-scale range C-reactive protein latex-enhanced immunoturbidimetry detection kit
  • Full-scale range C-reactive protein latex-enhanced immunoturbidimetry detection kit

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[0022] The main raw materials involved in this kit are as follows:

[0023] Rat anti-human CRP antibody (catalog number: NO501194), mouse anti-human CRP antibody (catalog number: NO501195), styrene latex (JSR catalog number: P0014, P0113), PEG (polyethylene glycol).

[0024] The preparation scheme of the main reagent of the present invention is as follows:

[0025] Reagent R1: 4% PEG (polyethylene glycol), 0.5% Nacl, 0.05% TritonX-100 phosphate buffer, the reagent is a colorless and transparent solution. Polyethylene glycol is a sensitizer, which can improve the analytical sensitivity of the reagent. Sodium chloride maintains the ionization strength of the reagent. TritonX-100 maintains the dispersion of latex particles and prevents the latex from self-aggregation.

[0026] Reagent R2: Use rat anti-human CRP antibody and mouse anti-human CRP antibody to sensitize two kinds of latex particles with a particle size of 80nm and 240nm respectively after mixing with a protein concentration ...

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Abstract

The invention discloses a full-scale range C-reactive protein latex-enhanced immunoturbidimetry detection kit. Through turbidity change, quantitative determination is realized. The kit comprises a reagent R1 and a reagent R2. The reagent R1 is a phosphate buffer solution. The reagent R2 comprises two conjugates with different particle sizes, and the conjugates comprise a conjugate of carboxylic polystyrene latex and a rat anti-human CRP antibody and a conjugate of carboxylic polystyrene latex and a mouse anti-human CRP antibody. The kit can be combined with a full-automatic specific protein analyzer so that detection sensitivity and accuracy are improved and detection time is shortened. The full-scale range C-reactive protein latex-enhanced immunoturbidimetry detection kit can detect high concentration CRP content and low concentration CRP content and has the advantages of high sensitivity, strong stability, wide linear range, good repeatability and cheap price.

Description

technical field [0001] The invention belongs to the field of medical immunology, and relates to an immunodetection reagent, in particular to a full-scale C-reactive protein latex-enhanced immune turbidimetric detection kit. Background technique [0002] C-reactive protein (C-reactiveprotein, CRP) is synthesized by hepatocytes, produced during fetal period, and transmitted by non-maternal placenta. Its production mechanism is: when the body is infected or the tissue is damaged, macrophages and other white blood cells are activated to produce interleukin-6 (IL-6), interleukin-1 (IL-1), tumor necrosis factor (TNF-a) and other cytokines and other mediators, these cytokines and mediators reach the liver, stimulate liver cells and epithelial cells to synthesize CRP. C-reactive protein can be divided into ordinary CRP, high-sensitivity CRP (hs-CRP), and full-scale CRP due to the different sensitivity of detection methods. Ordinary CRP is mainly used clinically to evaluate infecti...

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/68G01N2333/4737
Inventor 董梅杨子学
Owner NANJING WENZHI BIOTECH
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