Full-scale range C-reactive protein latex-enhanced immunoturbidimetry detection kit

Abstract

The invention discloses a full-scale range C-reactive protein latex-enhanced immunoturbidimetry detection kit. Through turbidity change, quantitative determination is realized. The kit comprises a reagent R1 and a reagent R2. The reagent R1 is a phosphate buffer solution. The reagent R2 comprises two conjugates with different particle sizes, and the conjugates comprise a conjugate of carboxylic polystyrene latex and a rat anti-human CRP antibody and a conjugate of carboxylic polystyrene latex and a mouse anti-human CRP antibody. The kit can be combined with a full-automatic specific protein analyzer so that detection sensitivity and accuracy are improved and detection time is shortened. The full-scale range C-reactive protein latex-enhanced immunoturbidimetry detection kit can detect high concentration CRP content and low concentration CRP content and has the advantages of high sensitivity, strong stability, wide linear range, good repeatability and cheap price.

Description

technical field [0001] The invention belongs to the field of medical immunology, and relates to an immunodetection reagent, in particular to a full-scale C-reactive protein latex-enhanced immune turbidimetric detection kit. Background technique [0002] C-reactive protein (C-reactiveprotein, CRP) is synthesized by hepatocytes, produced during fetal period, and transmitted by non-maternal placenta. Its production mechanism is: when the body is infected or the tissue is damaged, macrophages and other white blood cells are activated to produce interleukin-6 (IL-6), interleukin-1 (IL-1), tumor necrosis factor (TNF-a) and other cytokines and other mediators, these cytokines and mediators reach the liver, stimulate liver cells and epithelial cells to synthesize CRP. C-reactive protein can be divided into ordinary CRP, high-sensitivity CRP (hs-CRP), and full-scale CRP due to the different sensitivity of detection methods. Ordinary CRP is mainly used clinically to evaluate infecti...

AI Technical Summary

Problems solved by technology

The routine clinical method for measuring CRP is the immunoturbidimetric method, and the measuring range is generally 3-200 mg / L, but the sensitivity is relatively low, and the CRP content below 3 mg / L cannot be accurately measured. Therefore, it cannot be used as a risk indicator for cardiovascular diseases. Indicators to predict the risk of cardiovascular disease in healthy people in the future

The detection range of clinical ultra-sensitive CRP is 0.3-100mg / L, and the measurement range is relatively narrow, so it is impossible to accurately measure the content of CRP at high concentrations. Therefore, it cannot be used for the diagnosis of infectious diseases or observation of curative effect

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