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Obtaining method of wheat blossom midge

A technology of wheat midge and acquisition method, which is applied in animal husbandry and other fields, can solve the problems of not considering the influence of diapause release, obtaining non-diapause insect state, and not studying the influence of temperature and soil moisture content development, etc., achieving simplicity and convenience The effect of efficiency and low cost

Inactive Publication Date: 2015-12-23
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the existing research is to put the diapause larvae of wheat midges in the low temperature below 10 ℃ for 2 to 4 months, and then break the diapause by the high temperature above 20 ℃ to obtain the insect source; but these studies have not considered the effect of natural temperature change on diapause The impact of the removal of the diapause and the influence of temperature and soil moisture on the development after diapause have not been studied. At the same time, it is difficult to obtain a non-diapause state and establish a test population in a short period of time.

Method used

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  • Obtaining method of wheat blossom midge
  • Obtaining method of wheat blossom midge
  • Obtaining method of wheat blossom midge

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] From June 2013 to May 2014, an experiment on the effect of natural temperature change on the elimination of diapause of wheat midges was carried out. The specific steps are as follows:

[0019] (1) Establishment of insect nurseries: During the yellow ripening period of wheat in 2013, a large number of insect-susceptible wheat ears were collected from the re-infested areas of wheat midges in that year, and put into insect nurseries, so that mature larvae fell naturally and entered diapause. In order to prevent the death of insect sources caused by dry soil and high temperature, watering was irregular in summer, and the diapause larvae used in the experiment were all collected from the insect nursery.

[0020] (2) Collect soil containing diapause larvae of wheat midges: collect soil from the insect nursery respectively from June to late October, mid-November, December to early March and late May in 2014, with a depth of 20 cm. Put it into a woven bag and bring it back to ...

Embodiment 2

[0026] In January 2014, an experiment on the influence of temperature and soil moisture content on the elimination of diapause of wheat midges was carried out. The specific steps are as follows:

[0027] (1) Prepare soil with an absolute moisture content of 20%, 30%, 40%, 50% and 60% respectively, and ensure that the soil does not contain midges, and put them into flowerpots.

[0028] (2) collect the soil containing the overwintering diapause larvae of wheat midges from the insect nursery in embodiment 1, scour the soil to obtain diapause round cocoons, put into step (1) respectively the flowerpots with different water content soils in every 100, Put the round cocoons at a distance of 3cm from the surface, and then place the flower pots in an artificial climate chamber with a temperature of 18°C, 22°C, 26°C and 30°C, a relative humidity of 70±5%, and a photoperiod of 16L:8D. Each treatment is repeated 3 times.

[0029] (3) Eclosion observation and calculation: regularly obser...

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Abstract

The invention relates to an obtaining method of wheat blossom midge. The obtaining method comprises the following steps: (1), collecting soil containing diapause larva of wheat blossom midge; (2), placing the soil in a container, adding water to enable the absolute water content of soil to be 15-60%, and further placing in a manual climatic box for breeding with the temperature of 18-30 DEG C and the relative humidity of 70-80%; (3), elutriating soil to obtain free larva after cocoon-break of larva, further elutriating soil to obtain pupas after pupation; (5), collecting wheat blossom midge imagoes. The obtaining method provided by the invention is time-saving, simple, convenient, low in cost, and can obtain experimental population of wheat blossom midge that are consistent in growth and development in indoor condition.

Description

technical field [0001] The invention relates to a method for obtaining small pests, in particular to a method for obtaining wheat midges. Background technique [0002] Wheat midge (Sitodiplosis mosellana (Gehin)) is an important pest that occurs intermittently and rampantly in wheat production. It uses larvae to lurk in the glume and suck the juice of the grains that are filling, causing wheat grains to rot, empty shells or mildew, and generally reduce wheat production. 10% to 20%, 30% to 50% in severe cases, or even no grains, seriously affecting the yield and quality of wheat, and posing a serious threat to the safe production of wheat. [0003] Wheat midge is a typical obligate diapause insect, generally, one generation occurs in one year. After the wheat enters yellow maturity, mature larvae shed their ears and fall into the soil, mainly diapausing in the soil in the form of cocoons until the wheat turns green in March of the following year and resumes activities, and p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 成卫宁龙治任刘洋
Owner NORTHWEST A & F UNIV
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