Sweet and fragment natural plant essential oil soap with bacteriostasis function, and production method thereof
A natural plant essential oil, sweet and fragrant technology, applied in the field of daily chemical products, can solve the problems of irritation and corrosion
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Embodiment 1
[0020] Take 4ml of bitter neroli essential oil, 2ml of lavender essential oil, 3ml of melissa essential oil, 3ml of chamomile essential oil, and 1ml of frankincense essential oil in a glass or stainless steel container, mix them evenly, and ultrasonically oscillate for 10 seconds to obtain a mixed essential oil.
[0021] Heat the soap in a water bath at 55°C. After the soap base is dissolved, take 87ml of soap base and 13ml of mixed essential oil, mix them evenly in the container and pour them into a mold. After cooling, put the prepared essential oil soap in a refrigerator at 4°C Save for later.
[0022] Determination of antibacterial activity:
[0023] 1.1 Disc diffusion method
[0024] Culture the bacteria to be tested (Escherichia coli ATCC25922, Staphylococcus aureus subsp. 8 CFU mL -1 .
[0025] Pour the sterilized beef extract peptone agar medium into a 9cm-diameter petri dish. After the medium solidifies, use a pipette gun to draw 100 μl of each bacterial solution ...
Embodiment 2
[0038] Take 4ml of bitter neroli essential oil, 2ml of lavender essential oil, 2ml of melissa essential oil, 2ml of chamomile essential oil, and 2ml of frankincense essential oil, mix them evenly in a glass or stainless steel container, and ultrasonically oscillate for 10 seconds to obtain a mixed essential oil.
[0039]Heat the soap in a water bath at 60°C. After the soap base is dissolved, take 88 parts of the soap base and 12ml of mixed essential oil, mix them evenly in the container and pour them into a mold. After cooling, put the prepared essential oil soap into a refrigerator at 4°C Save it for later use.
[0040] Determination of antibacterial activity:
[0041] 1.1 Disc diffusion method
[0042] The bacterial species to be tested (Escherichia coli ATCC25922, Staphylococcus aureus subsp. Aureus ATCC25923, Pseudomonas aeruginosa) ATCC27583 were respectively cultured with beef extract peptone liquid medium, and each bacterial solution was diluted to 1×10 with a turbidim...
Embodiment 3
[0056] Take 1ml of bitter neroli essential oil, 3ml of lavender essential oil, 3ml of melissa essential oil, 3ml of chamomile essential oil, and 1 part of frankincense essential oil, mix them evenly in a glass or stainless steel container, and ultrasonically oscillate for 10 seconds to obtain a mixed essential oil.
[0057] Heat the soap in a water bath at 60°C. After the soap base is dissolved, take 89ml of soap base and 11ml of mixed essential oil, mix them evenly in the container and pour them into a mold. After cooling, put the prepared essential oil soap in a refrigerator at 4°C for storage spare.
[0058] Determination of antibacterial activity:
[0059] 1.1 Disc diffusion method
[0060] The bacterial species to be tested (Escherichia coli ATCC25922, Staphylococcus aureus subsp. Aureus ATCC25923, Pseudomonas aeruginosa) ATCC27583 were respectively cultured with beef extract peptone liquid medium, and each bacterial solution was diluted to 1×10 with a turbidimeter befor...
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