A modified sea snake antimicrobial peptide qha3 and its preparation method and application
An antibacterial peptide and antibacterial drug technology, applied in the field of biomedicine, can solve problems such as human health threats and antibiotic pathogenic microorganism resistance, and achieve the effects of broad-spectrum high-efficiency antibacterial activity, low hemolytic activity, and small molecular weight
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Embodiment 1
[0012] Chemical Synthesis of Antimicrobial Peptide QHA3 from Transformed Sea Snake
[0013] Qinghuan Sea Snake Antimicrobial Peptide QHA is a linear polypeptide encoded by a gene, containing 30 amino acid residues, with a molecular weight of 3628.5Da and an isoelectric point of 12.61. The complete sequence of Qinghuan sea snake antimicrobial peptide QHA is: Lys 1 Phe 2 Phe 3 Lys 4 Arg 5 Leu 6 Leu 7 Lys 8 Ser 9 Val 10 Arg 11 Arg 12 Ala 13 Val 14 Lys 15 Lys 16 Phe 17 Arg 18 Lys 19 Lys 20 Pro 21 Arg 22 Leu 23 Ile 24 Gly 25 Leu 26 Ser 27 Thr 28 Leu 29 Leu 30 . According to the amino acid sequence of Qinghuan sea snake antibacterial peptide QHA, the modified QHA3 was designed by molecular transformation method, and it was chemically synthesized by peptide solid-phase synthesis method. The specific preparation method is as follows:
[0014] I. Preparation method of QHA3: According to the above amino acid sequence of QHA3, its full sequence was synthesiz...
Embodiment 2
[0020] QHA3 pharmacological experiment:
[0021] 1. Determination of antibacterial activity of QHA3:
[0022] (1) Pick the test strains stored on the slope and evenly spread them on the MH solid medium (purchased from Qingdao Haibo Biotechnology Co., Ltd.) plate, and place the sterilized 0.5cm diameter filter paper on the medium On the surface, 10 μl of 2 mg / ml antibacterial peptide QHA3 sample solution dissolved in sterilized deionized water was added dropwise, and incubated upside down at 37°C for 18-20 hours to observe whether the inhibition zone was formed or not. If the sample has antibacterial activity, a clear and transparent bacteriostatic zone will be formed around the filter paper, and the larger the bacteriostatic zone, the stronger the antibacterial activity of the sample.
[0023] (2) Antimicrobial peptide QHA3 minimum inhibitory concentration (Minimum Inhibitory Concentration) determination (2-fold dilution method):
[0024] Select the strains with the inhibiti...
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