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Debaryomyces hansenii and application thereof in treatment for high-concentration ammonium sulfate industrial wastewater

An industrial wastewater, ammonium sulfate technology, applied in the food industry wastewater treatment, biological water/sewage treatment, water/sludge/sewage treatment and other directions, can solve the problems of microbial growth inhibition, death, etc., to solve wastewater pollution, realize resource effect of chemical utilization

Active Publication Date: 2016-01-27
ACAD OF NAT FOOD & STRATEGIC RESERVES ADMINISTRATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, it has also been reported that some microbial strains can treat low-concentration ammonium sulfate industrial wastewater, but when the concentration of ammonium sulfate exceeds 10%, the growth of microorganisms will be inhibited and even die

Method used

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  • Debaryomyces hansenii and application thereof in treatment for high-concentration ammonium sulfate industrial wastewater
  • Debaryomyces hansenii and application thereof in treatment for high-concentration ammonium sulfate industrial wastewater
  • Debaryomyces hansenii and application thereof in treatment for high-concentration ammonium sulfate industrial wastewater

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 Debaryomyceshansenii acquisition and identification

[0034]Sampling was taken from the waste liquid pool of the monosodium glutamate factory, screened by PDA plates (containing 15% ammonium sulfate), and single colonies with good separation and different colony shapes and colors were selected, and then inoculated in PDA liquid culture medium containing different concentrations of ammonium sulfate for observation For growth conditions, strains resistant to high concentrations of ammonium sulfate were screened; the screened strains resistant to high concentrations of ammonium sulfate were inoculated in monosodium glutamate waste liquid, and fermentation experiments were performed to verify the metabolic growth of each single colony in wastewater. Finally, a strain capable of utilizing ammonia nitrogen in wastewater was successfully obtained. Pick a single colony in PDA (containing 30% ammonium sulfate) liquid medium, and when it reaches the logarithmic phase...

Embodiment 2

[0040] The growth of embodiment 2ASAGFY31 in the PDA liquid medium containing different concentrations of ammonium sulfate

[0041] The activated ASAGFY31 single colony was inoculated in PDA liquid medium containing 15% ammonium sulfate, shaken and cultured at 28°C and 200rpm for 24h, and transferred to PDA liquid medium containing different concentrations of ammonium sulfate, so that the initial OD600 was Shake culture at 0.05, 28°C and 200rpm, take samples every 4h to measure OD600, the results show that: ASAGFY31 can grow well in PDA liquid medium containing 25% and 35% ammonium sulfate, and plate colonies after 48h and 72h of culture respectively Counting method (colony forming unit, cfu) detects that the number of viable bacteria reaches 10 8 CFU / mL (such as Figure 4 shown). In the PDA liquid culture medium of 50% ammonium sulfate, begin to grow after 96h, after 144h, plate colony counting method (colonyforming unit, cfu) detects that the number of viable bacteria reac...

Embodiment 3

[0042] Example 3 Comparison of tolerance between ASAGFY31 and CGMCC2.33 in PDA medium containing different concentrations of ammonium sulfate

[0043] Transfer single colonies of activated ASAGFY31 and CGMCC2.33 Debaria hansenii (Chinese Patent Publication No. CN103642706A) to ep tubes containing 1 mL of sterile water, shake evenly on a vortex mixer, and take 100 μL The suspension was coated on a PDA solid plate containing 15% ammonium sulfate, and cultured statically in a biochemical incubator at 28°C. After culturing for 3 days, ASAGFY31 covered the entire plate, and the number of colonies of CGMCC2.33 in the plate was significantly less than that of ASAGFY31 ( Such as Figure 5 shown). Further, two strains of Debaria hansenii after activation were respectively inoculated in PDA liquid medium containing 15% ammonium sulfate, and after shaking culture at 28°C and 200 rpm, they were respectively transferred to PDA containing 25% ammonium sulfate. In the liquid medium, the in...

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Abstract

The invention discloses a Debaryomyces hansenii and an application thereof in treatment for high-concentration ammonium sulfate industrial wastewater. The preservation No. of the Debaryomyces hansenii ASAGFY31 is CGMCC No. 11237. The invention also provides a method for utilizing the Debaryomyces hansenii to treat the high-concentration ammonium sulfate industrial wastewater. The Debaryomyces hansenii provided by the invention can be utilized to effectively solve the problem of wastewater pollution, a large amount of mycoprotein can be acquired and applied to the fodder and cultivation industry and the resource utilization of the wastes is realized.

Description

technical field [0001] The invention belongs to the technical field of microbial application, and relates to a strain of Debaria hansenii and its application in treating high-concentration ammonium sulfate industrial wastewater. Background technique [0002] High-concentration ammonium sulfate wastewater is a common type of industrial wastewater. Its ammonium sulfate concentration is high and ammonia nitrogen content is high. In order to prevent the excessive discharge of ammonia nitrogen from causing eutrophication of the water body and causing environmental pollution, this type of wastewater must be treated to meet the discharge standard before it can be discharged. emission. The most common high-concentration ammonium sulfate wastewater, such as monosodium glutamate wastewater, is a kind of difficult-to-treat high-concentration organic wastewater produced by the fermentation and production of monosodium glutamate in monosodium glutamate enterprises. It has high COD, high ...

Claims

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Application Information

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IPC IPC(8): C12N1/16C02F3/34C12R1/645C02F103/32C02F101/16
Inventor 陈园杨博磊赵晨张晓琳黎琪
Owner ACAD OF NAT FOOD & STRATEGIC RESERVES ADMINISTRATION
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