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A kind of preparation method of ivy leaf-solidified water body denitrification microbial preparation

A microbial preparation and creeper technology, which is applied in the field of preparation of creeper leaf-solidified water body denitrification microbial preparations, can solve the problems of greater influence on microbial activity, single application effect, and small number of effective viable bacteria, and achieve short processing time, The effect of large number of effective viable bacteria and low cost

Active Publication Date: 2017-08-08
海南正强超越生化技术开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention: the microbial activity is greatly affected by various environmental factors of the water body when the current microbial preparation is used to treat the aquaculture water body, it needs to be used for a long time, its properties are unstable during use, the number of effective viable bacteria is small, and most of the existing microbial preparation In order to introduce foreign common bacterial agents, which lead to the disadvantages of long treatment time, single application effect and high treatment cost, a preparation method of creeper leaf-solidified water body denitrification microbial preparation is provided. , take mannitol, tryptone and other substances to prepare microbial culture medium, put pond sludge in culture medium, mix with sterile water and filter the filtrate to inoculate on microbial culture medium, add nutrient solution for repeated cultivation, and aseptic The filtrate is obtained by mixing with water and filtering, centrifuged to obtain the denitrification biological strain, and then mixed with the spare creeper leaf scraps, glycerin and skim milk, dried and sieved to obtain the filtrate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0016] First, select the ivy leaves that grow in January in summer, remove them and wash them, expose them to the sun until they are completely withered and dehydrated, rub and squeeze them until the leaves of the ivy are completely separated from the main stem, and collect the broken leaves And place it in a jet mill to pulverize and sieve to obtain 100 mesh creeper leaf scraps for subsequent use; then, in parts by weight, select 42 parts of mannitol, 5 parts of tryptone, 18 parts of starch and 35 parts of agar , stir and mix to form a culture medium, and add deionized water to adjust the pH to 5.0. After the pH is adjusted, it is heated to 120 ° C for 10 minutes to sterilize to obtain a microbial culture medium; then select the pond sludge that needs to be treated, Place it in a cool place to air-dry until the water content is 60%, then place the air-dried pond sludge in the microbial culture medium prepared above at 20g / part, and cultivate it at 18°C ​​for 2 weeks, then cult...

example 2

[0019] First, select the ivy leaves that grow for 1.5 months in summer, remove them, wash them, expose them to the sun until they are completely withered and dehydrated, rub and squeeze them until the leaves of the ivy are completely separated from the main stem, and collect the broken leaves And place it in a jet mill to pulverize and sieve to obtain 110 mesh creeper leaf scraps for subsequent use; then, in parts by weight, select 45 parts of mannitol, 10 parts of tryptone, 15 parts of starch and 30 parts of agar , stir and mix to form a culture medium, and add deionized water to adjust the pH to 5.2. After the pH is adjusted, it is heated to 120 ° C for 15 minutes to sterilize to obtain a microbial culture medium; then select the pond sludge that needs to be treated, Place it in a cool place to air-dry until the water content is 65%, then place the air-dried pond sludge in the microbial culture medium prepared above at 20g / part, and cultivate it at 19°C for 2.5 weeks, then cu...

example 3

[0022] First, select the creeper leaves that grow in February in summer, remove them, wash them, expose them to the sun until they are completely withered and dehydrated, rub them and squeeze them until the leaves of the creeper are completely separated from the main stem, and collect the broken leaves And place it in a jet mill to pulverize and sieve to obtain 120 mesh creeper leaf scraps for subsequent use; then, in parts by weight, choose 35 parts of mannitol, 5 parts of tryptone, 20 parts of starch and 40 parts of agar , stir and mix to form a culture medium, and add deionized water to adjust the pH to 5.5. After the pH is adjusted, it is heated to 120°C for 20 minutes to sterilize to obtain a microbial culture medium; then select the pond sludge that needs to be treated, Place it in the shade and air-dry until the water content is 70%, then place the air-dried pond sludge in the microbial culture medium prepared above at 20g / part, and cultivate it for 3 weeks at 20°C, and ...

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Abstract

The invention discloses a preparation method of a creeper leaf-solidified water body denitrification microbial preparation, which belongs to the field of preparation of microbial preparations. The microbial preparation of the present invention is to take creeper leaves and process the broken leaves for later use, take mannitol, tryptone, starch and agar to prepare microbial culture medium, place the treated pond sludge in the culture medium, mix and filter with sterile water The obtained filtrate was inoculated on the microbial culture medium, added with nutrient solution for repeated cultivation, mixed with sterile water and filtered to obtain the filtrate, centrifuged to obtain denitrification biological strains, and then mixed with spare creeper leaf scraps, glycerin and skim milk, and passed through It is obtained by drying and sieving. The beneficial effects of the present invention are: the microbial preparation prepared by the present invention has stable properties, a large number of effective viable bacteria, is easy to store, and has a storage period of 10 to 18 months; the preparation method is simple, the cost is low, the processing time is short, and the denitrification The rate is more than 92%, which is worthy of popularization and use.

Description

technical field [0001] The invention relates to a preparation method of a creeper leaf solidified water body denitrification microbial preparation, belonging to the field of microbial preparation preparation. Background technique [0002] With the rapid development of the aquaculture industry, the aquaculture water body is becoming more and more eutrophic, which has caused great harm to the surrounding water environment and ecological environment. The non-standard use of antibiotics and fungicides in the breeding process, on the one hand, leads to the emergence of drug residues in the breeding waters, and then the emergence of various medicinal bacteria. destroy. Therefore, it is more and more urgent to restore the water body of aquaculture ponds, and the emerging industry of microbial preparations for aquaculture has become a key direction for the future development of aquaculture. Under the guidance of microecological theory, microecological preparations are physiologica...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/34
Inventor 朱行超郑德州罗圣馗
Owner 海南正强超越生化技术开发有限公司
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