Photo-curing hydrogel for multi-cell sorting and stem cell area-selecting differentiation and preparation method thereof

A multi-cell sorting and light-curing technology, applied in the field of biomedical materials, can solve problems such as discontinuity, and achieve the effects of good affinity, simple and easy-to-obtain raw materials, and low cost.

Active Publication Date: 2016-02-03
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current technology is limited to the limited area design of the topological structure of the substrate, the control of the hardness of the substrate material discontinuity, or the intervention of specific growth factors in the micropattern, and rarely involves the realization of different substrates directly on the surface of a single substrate. Regulation of regional hardness

Method used

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  • Photo-curing hydrogel for multi-cell sorting and stem cell area-selecting differentiation and preparation method thereof
  • Photo-curing hydrogel for multi-cell sorting and stem cell area-selecting differentiation and preparation method thereof
  • Photo-curing hydrogel for multi-cell sorting and stem cell area-selecting differentiation and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A photocurable hydrogel for multi-cell sorting and stem cell selection differentiation, prepared by the following method:

[0034] (1) Polymer modification: Dissolve 1 g of collagen in 0.5 M acetic acid solution at a concentration of 5 mg / ml, add 50 ml of acetone solution containing 10 ml of TEA and 10 ml of GMA, and stir magnetically for 3 days at 37 ° C; then mix Add the solution into a dialysis bag, place it in an external environment of ultrapure water, and change the ultrapure water every 6 hours to fully remove residual organic solvents and impurity ions. After 2 days of dialysis, freeze-dry the dialysis purified product and store it in the refrigerator- Store at 20°C for later use;

[0035](2) Preparation of a micropattern mask: the mask has a light-transmitting area in the shape of straight stripes with a width of 50 μm and a spacing of 50 μm, and the remaining areas are black opaque areas;

[0036] (3) The modified collagen obtained in step (1) was dissolved i...

Embodiment 2

[0040] A photocurable hydrogel for multi-cell sorting and stem cell selection differentiation, prepared by the following method:

[0041] (1) Polymer modification: Dissolve 1 g of collagen in 0.5 M acetic acid solution at a concentration of 5 mg / ml, add 150 ml of acetone solution containing 30 ml of TEA and 30 ml of GMA, and stir magnetically for 3 days at 37 ° C; then mix Add the solution into a dialysis bag, place it in an external environment of ultrapure water, and change the ultrapure water every 6 hours to fully remove residual organic solvents and impurity ions. After 2 days of dialysis, freeze-dry the dialysis purified product and store it in the refrigerator- Store at 20°C for later use;

[0042] (2) Preparation of a micropattern mask: the mask has a square array of light-transmitting areas with a side length of 200 μm and a spacing of 50 μm, and the remaining areas are black opaque areas;

[0043] (3) Dissolve the modified collagen obtained in step (1) in water at a...

Embodiment 3

[0046] A photocurable hydrogel for multi-cell sorting and stem cell selection differentiation, prepared by the following method:

[0047] (1) Polymer modification: Dissolve 1 g of hyaluronic acid in PBS buffer solution at a concentration of 10 mg / ml, add 150 ml of acetone solution dissolved in 30 ml of TEA and 30 ml of isocyanoethyl methacrylate, and magnetically Stir for 5 days; then add the mixed solution to 5 times the volume of acetone for precipitation, dissolve it with PBS, repeat this step 3 times to fully remove residual organic solvents and impurity ions, freeze-dry the purified product, and store it in the refrigerator at -20°C Save for later use;

[0048] (2) Preparation of a micropattern mask: the mask has a small square array light-transmitting area with a side length of 30 μm and a spacing of 50 μm, and the remaining areas are black opaque areas;

[0049] (3) Dissolve the modified hyaluronic acid obtained in step (1) in water at a concentration of 50mg / ml, add 1...

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Abstract

The invention belongs to the field of biomedical materials, and particularly relates to photo-curing hydrogel for multi-cell sorting and stem cell area-selecting differentiation and a preparation method thereof. The photo-curing hydrogel is prepared by taking modified high-polymer materials with the photo-curing performance as raw materials and are divided into multiple different areas by straight-stripe-shaped or net-stripe-shaped or annular-stripe-shaped micro-pattern structures, the hardness of the photo-curing hydrogel in the same area is same, the hardness of the photo-curing hydrogel in the different areas is same or different, and the overall photo-curing hydrogel at least has two kinds of the hardness. According to the photo-curing hydrogel for multi-cell sorting and stem cell area-selecting differentiation and the preparation method thereof, on one hand, the elasticity moduli of all the areas of the photo-curing hydrogel are different by controlling the ultraviolet radiation curing degree; on the other hand, all the areas with the different elasticity moduli present the micro-pattern structures on the photo-curing hydrogel, selective adhesion, proliferation and differentiation of cells on the photo-curing hydrogel can be achieved by combining the elasticity moduli with the micro-pattern structures, and therefore controllable cell sorting or stem cell area-selecting differentiation can be achieved.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a light-cured hydrogel for multi-cell sorting and stem cell differentiation and a preparation method thereof. Background technique [0002] Cell sorting refers to the method of screening and distinguishing cells from different species and different tissue types in a complex environment. Stem cell differentiation refers to the differentiation of stem cells in different regions at different levels and in different directions under the condition of external factors. Through the design of substrate materials, in vitro cell sorting and selective differentiation can be realized, that is, the complex environment in vivo can be simulated, so that co-cultured cells can grow in specific areas according to the design, or stem cells can be differentiated into various groups in the set area, In this way, complex multicellular tissue structures can be biomimetically formed in v...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F289/00C08F251/00C08F220/32C08F220/36C08F220/06C08F2/48C08J3/075C08J3/28C08L51/08C08L51/02C12N5/077C12N5/071
Inventor 张胜民杨高洁刘浩明胡茜茜
Owner HUAZHONG UNIV OF SCI & TECH
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