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A Quantitative Detection Method of Jiuwei Xifeng Granule Components and Its Fingerprint Construction Method

A technology of Jiuwei Xifeng and detection method, which is applied in the field of analytical chemistry to achieve the effect of good linear relationship, accurate detection and stable baseline

Active Publication Date: 2017-05-03
JIANGSU KANION PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no clear report on the quality control method of Jiuwei Xifeng Granules, so it is of great practical significance to provide a quality control method for Jiuwei Xifeng Granules

Method used

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  • A Quantitative Detection Method of Jiuwei Xifeng Granule Components and Its Fingerprint Construction Method
  • A Quantitative Detection Method of Jiuwei Xifeng Granule Components and Its Fingerprint Construction Method
  • A Quantitative Detection Method of Jiuwei Xifeng Granule Components and Its Fingerprint Construction Method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1 9

[0074] Example 1 Quantitative detection method for components of Jiuwei Xifeng Granules

[0075] 1. Chromatographic conditions and system adaptability test: Octadecylsilane bonded silica gel was used as filler (chromatographic column: Phenomenex GeminiC 18 , the column length is 250mm, the inner diameter is 4.6mm, and the particle diameter is 5μm); methanol is used as mobile phase A, and 0.1% triethylamine is used as mobile phase B, and the gradient elution is carried out according to the regulations in the table below; the flow rate is 1.0mL / min; the detection wavelength is 246nm; the column temperature is 35°C; the number of theoretical plates is not less than 5000 based on the rhynchophylline peak.

[0076] Table 1 Gradient elution program

[0077] time (minutes) Mobile phase A(%) Mobile phase B(%) 0～5 40 60 5～30 40→60 60→40 30～50 60→75 40→25 50～60 75 25

[0078] 2. Preparation of the reference substance solution: Accurately weig...

Embodiment 2 9

[0082] Example 2 Quantitative detection method for components of Jiuwei Xifeng Granules

[0083] 1. Chromatographic conditions and system adaptability test: Octadecylsilane bonded silica gel is used as filler (chromatographic column: Thermo syncronis C18 column, column length is 250mm, inner diameter is 4.6mm, particle size is 5μm); methanol Be mobile phase A, with 0.05% triethylamine as mobile phase B, carry out gradient elution according to the regulation in the table 1 of embodiment 1; Flow velocity is 1.2mL / min; Detection wavelength is 230nm; Column temperature 30 ℃; Number of theoretical plates Calculated according to rhynchophylline peak not less than 5000.

[0084] 2. Preparation of the reference substance solution: Accurately weigh the appropriate amount of the reference substance rhynchophylline, isorhynchophylline, dehydrorhynchophylline, and isodehydrorhynchophylline, add 80% ethanol to make 45.05 μg / mL of rhynchophylline , a mixed reference solution of isorhynchop...

Embodiment 3 9

[0087] Example 3 Quantitative Detection Method of Jiuwei Xifeng Granule Components

[0088] 1. Chromatographic conditions and system adaptability test: Octadecylsilane bonded silica gel is used as filler (chromatographic column: Agilent 5TC-C18 chromatographic column, column length is 250mm, inner diameter is 4.6mm, particle size is 5μm); Methanol is the mobile phase A, and 0.15% triethylamine is the mobile phase B, and carries out gradient elution according to the regulations in Table 1 of Example 1; the flow rate is 0.8mL / min; the detection wavelength is 260nm; the column temperature is 40°C; the theoretical plate The number is not less than 5000 based on the rhynchophylline peak.

[0089]2. Preparation of the reference substance solution: Accurately weigh the appropriate amount of the reference substance rhynchophylline, isorhynchophylline, dehydrorhynchophylline, and isodehydrorhynchophylline, add 80% ethanol to make 45.05 μg / mL of rhynchophylline , a mixed reference solu...

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Abstract

The present invention relates to the technical field of analytical chemistry, and particularly relates to a nine-element wind-extinguishing particle component quantitative detection method and a fingerprint construction method. The detection method comprises taking a nine-element wind-extinguishing particle to-be-tested sample and a standard substance for HPLC detection, and obtaining nine-element wind-extinguishing particle components and contents according to HPLC detection results; wherein HPLC detection chromatographic conditions are as follows: a C18 chromatographic column is used, methanol is used as a mobile phase A, and triethylamine is used as a mobile phase B for gradient elution. The fingerprint measured by the detection method can more fully reflect nine-element wind-extinguishing particle chemical components, chromatographic peak separation is good, baseline is steady, peak forms are good, reproducibility is good, the method has good linear relationship, precision, stability and recovery rate, and can accurately detect the nine-element wind-extinguishing particle chemical component content, and the obtained control fingerprint can objectively assess the quality of the sample.

Description

technical field [0001] The invention relates to the technical field of analytical chemistry, in particular to a method for quantitatively detecting components of Jiuweixifeng granules and a method for constructing fingerprints thereof. Background technique [0002] Tourette syndrome, also known as Tourette's syndrome or Tourette's syndrome, is a hereditary neuropsychiatric disorder characterized by chronic, fluctuating, multiple motor tics accompanied by involuntary vocalizations. Tourette syndrome has become relatively common, and many children come to see a doctor every year in primary hospitals alone. In recent years, the incidence of tourette syndrome has a tendency to increase significantly. Epidemiological surveys show that the annual incidence of Tourette's is generally 0.5 to 1 / 100,000 (the number of new patients per 100,000 per year). The incidence of Tourette's in males is significantly more than that in females, and the results reported in the literature are quit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 萧伟付娟李家春黄文哲王振中周习
Owner JIANGSU KANION PHARMA CO LTD
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