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A kind of primary culture method of lotus root stem tip tissue

A primary culture and tissue technology, applied in the fields of agriculture and biology, can solve the problems of high mutation rate, low seedling rate, and inability to elongate petiole, so as to speed up expansion, prevent mutation, and ensure purity

Active Publication Date: 2017-12-22
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the problems existing in the first generation of lotus root stem tip culture are: the shoot tip tissue is prone to high browning rate after inoculation; low rate

Method used

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  • A kind of primary culture method of lotus root stem tip tissue
  • A kind of primary culture method of lotus root stem tip tissue
  • A kind of primary culture method of lotus root stem tip tissue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0040] 1. Collection of buds: Three varieties of lotus root, 'Elian 4', 'Meirenhong' and 'Piaohua' were selected for cultivation. In order to ensure the purity of the variety, the bud collection is carried out in the variety garden, and the collection time is from May to August. The terminal buds of the hypertrophic underground stems are selected, and the length of the buds in the field sampling is 5-8cm.

[0041] 2. Sprout treatment: wash the collected sprouts with tap water for 15-20 minutes, soak them in 400 times 84 disinfectant for 10-15 minutes, and then rinse them with distilled water for 10-15 minutes.

[0042] 3. Disinfection and inoculation: both disinfection and inoculation are carried out in the ultra-clean workbench

[0043] 3.1 Disinfection:

[0044] 3.1.1 Cut the above-mentioned buds 3-5cm from the top.

[0045] 3.1.2 Disinfect with 75% alcohol for 15-20 seconds;

[0046] 3.1.3 Sodium hypochlorite solution with an effective chlorine concentration of 1%, disin...

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Abstract

The invention discloses a method for primary culture of lotus root stem tip tissue. The method comprises the following steps: 1) collection of lotus root buds; 2) pretreatment of buds; 3) disinfection and inoculation; 4) expansion culture of shoot tip tissue; 5) leaf expansion culture. The present invention prevents browning of explant tissue by adopting suitable culture medium and culture method; prevents deformity and variation of leaves by adopting leaf-expanding culture medium, so that stems and leaves develop normally. The method can make the seedling formation rate of the top meristem of the shoot tip of the lotus root reach more than 90%.

Description

technical field [0001] The invention relates to a primary culture method of lotus root stem tip tissue, belonging to the fields of agriculture and biotechnology. Background technique [0002] Plant tissue culture refers to separating the required tissues, organs or cells, protoplasts, etc. from the plant, and cultivating them under artificial control conditions through aseptic operation to obtain regenerated complete plants or produce other products with economic value. technology. This technology has broad application prospects in rapid propagation of rare plants or plants of high economic value, virus removal from plant bodies and preservation of plant germplasm resources. [0003] Lotus root is a vegetative plant, the parent plant is easy to produce underground stems, and the nodes of the rhizomes can produce new plants and reproduce offspring. This method of reproduction is easy to pass on the diseases and physiological disorders of the mother to the offspring, and it ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 高红胜陈学好李良俊王悠陈立宝
Owner YANGZHOU UNIV
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