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Culture solution for fresh water navicula and culture method

A Navicula and culture medium technology, applied in the field of algae cultivation, can solve the problems of difficulty in meeting the needs of aquaculture, unfavorable large-scale cultivation of Navicula, slow growth rate of Navicula, etc., and achieves the promotion of rapid division and proliferation with strong pertinence. , the effect of promoting rapid growth

Inactive Publication Date: 2016-02-24
YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, in natural water bodies, navicula grows mixed with other algae, and the population density is very low, which is difficult to meet the needs of aquaculture
At present, the cultivation of freshwater Navicula mostly adopts CSI medium. In this medium, the growth rate of Navicula is relatively slow, which is not conducive to the large-scale cultivation of Navicula.

Method used

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  • Culture solution for fresh water navicula and culture method
  • Culture solution for fresh water navicula and culture method
  • Culture solution for fresh water navicula and culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A kind of freshwater navicula culture fluid (the embodiment of the invention or claims LXN culture fluid), comprising:

[0021]

[0022]

[0023] The rest is double distilled water.

Embodiment 2

[0025] A method for cultivating freshwater navicula, comprising:

[0026] (1) Prepare LXN culture solution according to the formula described in Example 1, and adjust the pH value to 8. Put it in a sterilizing pot, sterilize at 120°C for 20 minutes. After sterilization, put it in a 4°C refrigerator for later use.

[0027] (2) Cultivate Navicula in CSI medium ("Handbook of Microalgal Culture") to logarithmic phase, and set aside.

[0028] (3) Perform starvation culture on Navicula in (2) for 1 d (the culture medium is a CSI medium without nitrogen, phosphorus, silicon, and iron), and set aside.

[0029] (4) Centrifuge 4000r / min Navicula through starvation culture for 10min, discard the supernatant and inoculate respectively in conical flasks equipped with LXN nutrient solution (test group) and CSI nutrient solution (control group), the inoculum size is 0.52 ×10 5 cells / mL, and seal with parafilm.

[0030] (5) Move the Erlenmeyer flask into a light incubator, set the temper...

Embodiment 3

[0040] A method for cultivating freshwater navicula, comprising:

[0041] (1) Prepare the LXN culture solution according to the formula described in Example 1, adjust the pH value to 7.5, put it into a sterilizer, and sterilize it at 120° C. for 20 minutes. After the sterilization is completed. Put it in a 4°C refrigerator for later use.

[0042] (2) Cultivate Navicula in CSI medium to logarithmic phase, and set aside.

[0043] (3) Perform starvation culture on Navicula in (2) for 1 d (the culture medium is a CSI medium without nitrogen, phosphorus, silicon, and iron), and set aside.

[0044] (4) Centrifuge 4000r / min Navicula through starvation culture for 10min, discard the supernatant and inoculate respectively in conical flasks equipped with LXN nutrient solution (test group) and CSI nutrient solution (control group), the inoculum size is 0.52 ×10 5 cells / mL, and seal with parafilm.

[0045] (5) Move the Erlenmeyer flask into a light incubator, set the temperature at 25...

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Abstract

The invention discloses a culture solution for fresh water navicula and a culture method. Formula components of the culture solution are simple and easy to obtain, according to fresh water navicula growth demand characteristics for nutritive salt, temperature, pH value and the like, the culture solution can promote rapid proliferation of the navicula effectively, promote the navicula to absorb and use various nutrients better, accelerate metabolism of navicula cells, increase the production of the navicula and achieve the purpose of promoting rapid growth of the pure-breed fresh water navicula. The culture solution is combined with the culture method. The culture method is simple to operate, high in pertinence and suitable for rapid growth of the fresh water navicula, has broad market prospect and is particularly suitable for being popularized and applied in the field of aquaculture.

Description

technical field [0001] The invention relates to the field of algae culture, in particular to a freshwater navicula culture solution and a culture method. Background technique [0002] Navicula is a genus of diatoms and is a single-celled algae commonly used for shellfish farming. It has a high fat content and rich types of fatty acids. The results of GC-MS analysis showed that the algae was rich in various unsaturated fatty acids. At the same time, the algal strain can secrete sulfated polysaccharides with various biological activities during the growth process. Therefore, Navicula is not only an ideal opening bait for aquatic animals, but also a planktonic algae with potential pharmaceutical value. [0003] However, in natural water bodies, navicula grows mixed with other algae, and the population density is very low, which is difficult to meet the needs of aquaculture. At present, the cultivation of freshwater Navicula mostly adopts CSI medium, in which the growth rate ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 李晓莉李谷陶玲
Owner YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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