A kind of plant extract compound probiotic time-sharing fermentation culture method

A technology of compounding probiotics and plant extracts, applied in the field of microbial fermentation, can solve the problem of less animal bifidobacteria, and achieve the effect of increasing the quantity

Active Publication Date: 2019-03-15
JINAN WEISHENGUI BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at the deficiencies in the above-mentioned prior art, the present invention provides a method for time-sharing fermentation and cultivation of plant extract compound probiotics, which greatly improves the production of Bifidobacterium animalis. Quantity, solved the technical problem of too little bifidobacteria in the fermentation process of compound probiotics, and enhanced the health effect of compound probiotics

Method used

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  • A kind of plant extract compound probiotic time-sharing fermentation culture method
  • A kind of plant extract compound probiotic time-sharing fermentation culture method

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Experimental program
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Embodiment 1

[0030] Embodiment 1: A time-sharing fermentation culture method of plant extract compound probiotics, comprising the following steps: (hereinafter, "F" plus "time" indicates the fermentation time from inoculation to the end of fermentation)

[0031] Step 1: Preparation of primary seed solution

[0032] Weigh 6g of yeast extract powder, 12g of glucose, 12g of sucrose, 3g of sodium chloride, 1.2g of dipotassium hydrogen phosphate, 0.3g of magnesium sulfate, and 517.5g of purified water to prepare 600ml of primary seed medium, and distribute it evenly to 6 triangles In the bottle, sterilize at a temperature of 121°C for 30 minutes, and cool the sterilized primary seed medium to 37°C for use;

[0033] The primary seed medium was inoculated with frozen strains: inoculated with Bifidobacterium animalis at F0h, Streptococcus thermophilus and Lactobacillus rhamnosus at F4h, placed in a constant temperature incubator at 37°C, and cultured statically; the culture time was 24h At F24h, ...

Embodiment 2

[0043] Embodiment 2: A time-sharing fermentation culture method of plant extract compound probiotics, comprising the following steps: (hereinafter, "F" plus "time" indicates the fermentation time from inoculation to the end of fermentation)

[0044] Step 1: Preparation of primary seed solution

[0045] Weigh 6g of yeast extract powder, 12g of glucose, 12g of sucrose, 3g of sodium chloride, 1.2g of dipotassium hydrogen phosphate, 0.3g of magnesium sulfate, and 517.5g of purified water to prepare 600ml of primary seed culture medium, and evenly distribute to 6 100ml In the triangular flask, sterilize at a temperature of 121°C for 30 minutes, and cool the sterilized primary seed medium to 37°C for later use;

[0046] The primary seed medium was inoculated with frozen strains: Bifidobacterium animalis was inoculated at F0h, Streptococcus thermophilus and Lactobacillus rhamnosus were inoculated at F8h, placed in a constant temperature incubator at 37°C, and cultured statically; the...

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Abstract

The invention discloses a time-sharing fermentation culture method of plant extract compound probiotics. The method includes the steps of first-level seed liquid preparation, second-level seed liquid preparation, fermentation medium preparation, fermentation and the like. In the fermentation and inoculation processes, a time-sharing inoculated fermentation method is adopted, and the problem that the content of bifidobacterium animalis is low is solved. By means of the method, the content of the bifidobacterium animalis in fermentation liquid is 1.0-3*108 CFU / ml; compared with a fermentation method in the prior art, the number of the bifidobacterium animalis is raised by about100 times, and the health efficacy of the compound probiotics is enhanced.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a method for time-sharing fermentation and cultivation of plant extract compound probiotics. Background technique [0002] Probiotics are a class of active microorganisms that are beneficial to the host. They are a general term for active beneficial microorganisms that colonize the human intestinal tract and reproductive system and can produce definite health effects, thereby improving the host's micro-ecological balance and exerting beneficial effects. Probiotics that can be used in food include Streptococcus, Lactobacillus, Bifidobacterium animalis, etc. [0003] Plant extract compound probiotic fermented drink refers to a drink prepared by fermenting with plant extract and inoculated with several different probiotic bacteria. [0004] In the prior art, since the growth conditions of the three probiotics of the genus Streptococcus, Lactobacillus, and Bifidobac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/01C12R1/46C12R1/225
Inventor 汤传忠
Owner JINAN WEISHENGUI BIOLOGICAL TECH CO LTD
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