Antioxidant or skin whitening composition containing 21-o-angeloyl theophylsaponol e3 derived from green tea seeds
An angeloyl teasapogenol, 21-O- technology is applied to the field of antioxidant or skin whitening compositions containing 21-O-angelioyl teasapogenol E3 derived from green tea seeds, and can solve usage limitations, inconveniences Sufficient whitening effect Skin stability problems, formulation and stability problems, to achieve the effect of inhibiting melanin production and excellent skin whitening effect
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Embodiment 1
[0048] [Example 1]: the preparation of green tea seed extract
[0049] Add 6 L of hexanoic acid to 2 kg of green tea seeds, degrease by stirring and extracting at room temperature 3 times, add 4 L of 50% ethanol to 1 kg of defatted green tea seeds, reflux extraction 3 times, and precipitate at 15°C 1 day. Then, filter through filter cloth and centrifuge to separate the residue from the filtrate, then suspend the extract obtained by concentrating the separated filtrate under reduced pressure in water, extract 5 times with 1 L of ether to remove the pigment, and wash the water layer with 500ml of 1-butanol extracted 3 times. The entire 1-butanol layer thus obtained was concentrated under reduced pressure to obtain a 1-butanol extract, and the 1-butanol extract obtained above was dissolved in a small amount of methanol, and a large amount of ethyl acetate was added for precipitation. The precipitate was dried, thereby obtaining 300 g of a green tea seed extract (crude saponin e...
Embodiment 2
[0050] [Example 2]: Prepare 21-O-angeloyl tea saponol E3 by acid hydrolysis method
[0051] [2-1] Add 20 times (v / w) 1N HCl-50% methanol solution (v / v) to 10 g of the green tea seed extract obtained in Example 1 above, and heat in a water bath at 80°C Reflux for 8 hours to hydrolyze the sugar bound to the crude saponins of green tea seeds. The reaction liquid was concentrated under reduced pressure to remove the solvent, and then ethanol (200 ml) was added to the residue and stirred 3 times, and the precipitated salt was removed by filtration. The filtered filtrate was concentrated under reduced pressure to obtain a crude product, and then the crude product obtained above was separated by silica gel column chromatography (chloroform:methanol=7:1~3:1), thereby obtaining 0.55 g of 21-O- Angelica Acid Tea Saponol E3.
[0052] [2-2] To 10 g of the green tea seed extract obtained in Example 1 above was added 20 times (v / w) of 1MH 2 SO 4 -30% aqueous solution (v / v), heated to re...
Embodiment 3
[0054] [Example 3] Prepare 21-O-angeloyl tea saponol E3 by alkali hydrolysis
[0055] [3-1] 10 g of the green tea seed extract obtained in Example 1 above was dissolved in anhydrous pyridine (500 ml), and sodium methoxide (powder, 10 g) was added thereto, and in an oil bath Reflux reaction for 8 hours to hydrolyze the sugar bound to the crude saponins of green tea seeds. The reaction solution was concentrated under reduced pressure to remove the solvent, washed with purified water three times, and filtered to obtain a filtrate. Ethanol (200 ml) was added to the residue, stirred three times, and the precipitated salt was removed by filtration. The filtered filtrate was concentrated under reduced pressure to obtain a crude product, and then the crude product obtained above was separated by silica gel column chromatography (chloroform:methanol=8:1~3:1), thereby obtaining 0.35 g of 21-O- Angelica Acid Tea Saponol E3.
[0056] [3-2] 10 g of the green tea seed extract obtained in ...
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