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Tissue Culture Rapid Propagation Method of Black Tiger

A tissue culture and tiger technology, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of not yet using leaves, few seeds, endangered wild resources, etc., and achieve easy transplanting, survival, and cultivation Short cycle and good genetic stability

Inactive Publication Date: 2017-04-19
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For a long time, black tiger medicinal materials have been completely dependent on wild resources. At the cost of digging the whole plant, the reproduction of the plant is mainly based on seed reproduction. Due to the poor firmness of the plant, wild animals love to eat its fruit, so the stock of seeds is small and the germination rate is low. low, resulting in a very slow reproduction rate, what's more, folk extinct excavation has caused wild resources at home and abroad to be endangered
At present, there are few reports on the tissue culture of the black tiger, especially the use of leaves as explants.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A method for rapid propagation of black tiger through tissue culture, comprising the following steps:

[0034] A, selection and processing of explants: get the young and tender leaves near the top of the stem tip of the black tiger, and get aseptic explants after disinfection;

[0035] B. Induction of explants: Remove the vein tissue of the sterile explants with a blade, cut into 1.0cm square leaf pieces, scratch the edge with a blade, inoculate into the induction medium, and culture at a temperature of 24°C in the dark. Cultivate under conditions for 7 days, then induce and cultivate for 21 days under the conditions of light intensity 1500Lux and light time 10 hours / day to obtain the first callus, wherein the formula in the induction medium is: MS, TDZ 1.0mg / L, 2 , 4-D 1.0mg / L, NAA 0.1mg / L, agar 4.5g / L and sucrose 25g / L, adjust the pH value of the induction medium to 5.8;

[0036] C, anti-browning treatment of callus: the first callus is transferred to the anti-browni...

Embodiment 2

[0044] A method for rapid propagation of black tiger through tissue culture, comprising the following steps:

[0045] A, selection and processing of explants: get the young and tender leaves near the top of the stem tip of the black tiger, and get aseptic explants after disinfection;

[0046] B. Induction of explants: Remove the vein tissue of the sterile explants with a blade, cut into 1.5cm square leaf pieces, scratch the edge with a blade, inoculate into the induction medium, and culture at a temperature of 25°C in the dark. Cultivate under conditions for 7 days, and then induce and cultivate for 21 days under the conditions of light intensity 1500Lux and light time 11 hours / day to obtain the first callus, wherein the formula in the induction medium is: MS, TDZ 1.25mg / L, 2 , 4-D 2.0mg / L, NAA 0.5mg / L, agar 4.5g / L and sucrose 25g / L, adjust the pH value of the induction medium to 5.8;

[0047] C, anti-browning treatment of callus: the first callus is transferred to the anti-b...

Embodiment 3

[0055] A method for rapid propagation of black tiger through tissue culture, comprising the following steps:

[0056] A, selection and processing of explants: get the young and tender leaves near the top of the stem tip of the black tiger, and get aseptic explants after disinfection;

[0057] B. Induction of explants: Remove the vein tissue of the sterile explants with a blade, cut into 2.0cm square leaf pieces, scratch the edge with a blade, inoculate into the induction medium, and culture at a temperature of 26°C in the dark. Under the conditions of culture for 7 days, and then induced culture under the conditions of light intensity 1500Lux and light time 12 hours / day for 21 days, the first callus was obtained, wherein the formula in the induction medium was: MS, TDZ 1.5mg / L, 2 , 4-D 3.0mg / L, NAA 1.0mg / L, agar 4.5g / L and sucrose 25g / L, adjust the pH value of the induction medium to 5.8;

[0058] C, anti-browning treatment of callus: the first callus is transferred to the an...

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Abstract

The invention discloses a tissue culture and rapid propagation method for kadsura coccinea. The method includes the step A of selection and processing of explants, the step B of induction of explants, the step C of browning prevention processing of calluses, the step D of differentiation of calluses, the step E of subculture multiplication culture, the step F of strong seedling culture, the step G of rooting culture and the step H of seedling hardening and transplanting, wherein in the step A, young and tender leaves close to tips of kadsura coccinea stems are taken and disinfected to obtain sterile explants. By means of the method, the browning rate of explants of kadsura coccinea is controlled at 7% or below, the rooting rate reaches 95%, and the seedling formation rate reaches 97% or above.

Description

technical field [0001] The invention relates to the field of black tiger breeding. More specifically, the present invention relates to a method for rapid propagation of black tigers through tissue culture. Background technique [0002] The black tiger is Kadsura coccinea (Lem) A.C.Smith, a magnolia plant. It is also called Zhuanlangfeng, Dazuan, Lengfantuan, etc. It is born in valleys and sparse forests. It has analgesic, sedative, and anti-inflammatory effects. It is used in Chinese medicine to treat rheumatic bone pain, bruises, stomach pain, acute enteritis, hernia, postpartum abdominal pain, and dysmenorrhea. It has been listed as a protected variety of traditional Chinese medicine for many times. Black Tiger is a perennial woody evergreen vine with lush stems, green leaves all year round, and good shading effect. It is suitable for interplanting shade medicinal materials. Its young fruits are green and dark red when mature, which is very ornamental, so it is very popu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 韦荣昌潘丽梅白隆华覃芳黄芩芬冯世鑫陈乾平翟勇进吴庆华
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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