Chinese yew cell culture medium

Abstract

The invention provides a Chinese yew cell culture medium. According to the culture medium, sodium nitrate and dipotassium phosphate serve as a nitrogen source and a phosphorus source respectively, a certain amount of calcium chloride is added, and on the basis, it is accidentally found that cell culture density is easily improved by adding traditional Chinese medicine materials of specific varieties and with specific components. On the basis of the beneficial discoveries, abelmoschus manilhot, edelweiss, Japan clover herb, clinopodium herb, wintersweet flowers, quassia, flaccid knotweed herb, selaginella moellendorfii hieron, polygala japonica and other traditional Chinese medicine components used for human disease treatment are preferably selected through experiment means, the obtained culture medium accidentally achieves an outstanding culture effect, and the culture density of Chinese yew cells is remarkably increased. Meanwhile, the growth speed of the Chinese yew cells is higher. By means of the innovative technical means, an excellent technical effect is obtained. Meanwhile, the cost is low, and the culture medium is easy to obtain and thus has good application prospects.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a yew cell culture medium. Background technique [0002] Yew, also known as yew, also known as Chinese yew, is a national first-class rare and protected tree species in China, and is also recognized as an endangered natural rare anti-cancer plant in the world. [0003] Paclitaxel and its analogues only exist in the genus Taxus and Australian Taxus in the gymnosperm Taxaceae. There are 11 species of Taxus in the world, which are widely distributed in the frigid, temperate and subtropical regions of Eurasia and North America. area, but is scattered and slow-growing. Taxus genus Australia is only distributed in the southern hemisphere, and its quantity is rare. The content of paclitaxel in yew plants is generally very low, even if it is less than 0.1% in the bark of yew yew, which is currently recognized as having the highest content, but it is in great demand. [0004] In or...

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the technical defects of the prior art, and provides a yew cell culture medium to solve the technical problem of the low density of the yew cell culture in the plant cell culture medium in the prior art