Chinese yew cell culture medium

The technology of taxus chinensis cells and culture medium is applied in the field of taxus chinensis cell culture medium, which can solve the problems of low culturing density of taxus chinensis cells and the like, and achieve the effects of good application prospect, easy realization and superior technical effect.

Inactive Publication Date: 2016-03-16
天津市博爱生物药业有限公司
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  • Summary
  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the technical defects of the prior art, and provides a yew cell culture medium to

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A yew cell culture medium, consisting of the following ingredients in parts by weight: 8 parts of sodium nitrate, 4 parts of dipotassium hydrogen phosphate, 2 parts of magnesium sulfate, 1 part of calcium chloride, 1 part of peptone, 1 part of hollyhock, 1 part of edelweiss 1 part of corn grass, 1 part of scissors grass, 1 part of wintersweet flower, 50 parts of water.

Embodiment 2

[0020] A yew cell culture medium, which is composed of the following ingredients in parts by weight: 10 parts of sodium nitrate, 6 parts of dipotassium hydrogen phosphate, 3 parts of magnesium sulfate, 2 parts of calcium chloride, 2 parts of peptone, 2 parts of hollyhock, 2 parts of edelweiss 2 parts of clam, 2 parts of corn grass, 2 parts of scissors grass, 2 parts of wintersweet flower, 60 parts of water.

Embodiment 3

[0022] A yew cell culture medium, consisting of the following ingredients in parts by weight: 9 parts of sodium nitrate, 5 parts of dipotassium hydrogen phosphate, 2.3 parts of magnesium sulfate, 1.8 parts of calcium chloride, 1.5 parts of peptone, 1.6 parts of hollyhock, 1.7 parts of edelweiss 1.8 parts of corn grass, 1.2 parts of clippers, 1.8 parts of wintersweet flower, 55 parts of water.

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PUM

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Abstract

The invention provides a Chinese yew cell culture medium. According to the culture medium, sodium nitrate and dipotassium phosphate serve as a nitrogen source and a phosphorus source respectively, a certain amount of calcium chloride is added, and on the basis, it is accidentally found that cell culture density is easily improved by adding traditional Chinese medicine materials of specific varieties and with specific components. On the basis of the beneficial discoveries, abelmoschus manilhot, edelweiss, Japan clover herb, clinopodium herb, wintersweet flowers, quassia, flaccid knotweed herb, selaginella moellendorfii hieron, polygala japonica and other traditional Chinese medicine components used for human disease treatment are preferably selected through experiment means, the obtained culture medium accidentally achieves an outstanding culture effect, and the culture density of Chinese yew cells is remarkably increased. Meanwhile, the growth speed of the Chinese yew cells is higher. By means of the innovative technical means, an excellent technical effect is obtained. Meanwhile, the cost is low, and the culture medium is easy to obtain and thus has good application prospects.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a yew cell culture medium. Background technique [0002] Yew, also known as yew, also known as Chinese yew, is a national first-class rare and protected tree species in China, and is also recognized as an endangered natural rare anti-cancer plant in the world. [0003] Paclitaxel and its analogues only exist in the genus Taxus and Australian Taxus in the gymnosperm Taxaceae. There are 11 species of Taxus in the world, which are widely distributed in the frigid, temperate and subtropical regions of Eurasia and North America. area, but is scattered and slow-growing. Taxus genus Australia is only distributed in the southern hemisphere, and its quantity is rare. The content of paclitaxel in yew plants is generally very low, even if it is less than 0.1% in the bark of yew yew, which is currently recognized as having the highest content, but it is in great demand. [0004] In or...

Claims

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Application Information

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IPC IPC(8): C12P17/02C12N5/04
CPCC12P17/02C12N5/04C12N2500/05C12N2500/30C12N2500/76
Inventor 夏雪城黄友法
Owner 天津市博爱生物药业有限公司
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