LC-MS/MS method for detecting nicotine and its metabolite in saliva

A technology for nicotine and metabolites, applied in the field of analysis and detection, can solve problems such as measurement errors, and achieve the effects of high sensitivity, non-invasive collection, and convenient collection

Inactive Publication Date: 2016-03-23
SHANGHAI DIAN CLINICAL TESTING CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The collection of urine is less invasive than that of blood, and the content of cotinine in urine is...

Method used

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  • LC-MS/MS method for detecting nicotine and its metabolite in saliva
  • LC-MS/MS method for detecting nicotine and its metabolite in saliva
  • LC-MS/MS method for detecting nicotine and its metabolite in saliva

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Experimental program
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Effect test

Embodiment 1

[0033] 1. Materials

[0034] The samples of the methodological research experiment came from the saliva samples of the employees of Shanghai Dean Medical Laboratory.

[0035] (1) Instrument: API4000 + Triple quadrupole mass spectrometer (ABSCIEX company); ekspert'ultraLC100 liquid chromatography system (with automatic sampler); medical high-speed refrigerated centrifuge (Beijing Baiyang Medical Equipment Co., Ltd.); ultrapure water instrument (Chengdu Youpuchao) Pure Technology Co., Ltd.); multi-tube vortex mixer (Hangzhou Aosheng Instrument Co., Ltd.); fast mixer (Jiangyan Kangtai Medical Equipment Factory); adjustable pipette (ThermoScientific0.5~10μL, 10~100μL , 100~1000μL); glassware, beakers, measuring cylinders, etc.

[0036] (2) Reagent consumables: chromatographically pure methanol and acetonitrile (Tedia High Purity Solvent Company.Inc); Shanghai Yuexu Technology chromatographic column UltimateSiO2 (2.1×100mm, 3μm); 1.5mL centrifuge tube (AXYGEN, USA).

[0037] (3)...

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Abstract

The invention discloses an LC-MS/MS method for detecting nicotine and its metabolites in saliva. The metabolites can be cotinine and 3-hydroxyl cotinine. LC-MS/MS is utilized to respectively detect a standard substance solution and a pretreated saliva sample, an isotope internal standard quantitative method is utilized to establish a calibration curve and calculate the concentration of the nicotine and its metabolites in the saliva with the concentration ratio of the standard substance solution and the internal standard substance as the X axis and the peak area ratio of the standard substance solution and the internal standard substance as the Y axis. The method is high in sensitivity, good in specificity, accurate and simple in pretreatment process and can complete separation and detection within 5 minutes, the precision and the stability meet the requirements, saliva samples are convenient to collect, no wound is caused, and the method can be used for evaluation of smoking and smoking cessation behaviors and research of environment smoke exposure degree.

Description

technical field [0001] The invention relates to a method for detecting nicotine and its metabolites in saliva by LC-MS / MS, belonging to the technical field of analysis and detection. Background technique [0002] Nicotine is an important component in tobacco. Both active smoking and passive smoking will have adverse effects on the body, such as cardiovascular disease, chronic obstructive pulmonary disease and even cancer. Smoking addiction has become a prominent status quo that endangers public health in today's society. The main metabolites of nicotine in the human body include various substances such as cotinine and 3-hydroxy cotinine, among which cotinine accounts for 70%-80% of all metabolites. According to reports, the concentration levels of nicotine and its metabolites in different body fluids (blood, urine, and saliva) are closely related to smoke exposure, and their levels can evaluate smoking, smoking cessation behavior, and environmental smoke exposure. Therefore...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 周亚飞于嘉屏
Owner SHANGHAI DIAN CLINICAL TESTING CENT
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