LC-MS/MS kit for detecting nicotine and its metabolites in saliva

A technology for nicotine and metabolites, applied in the field of analysis and detection, can solve problems such as measurement errors, and achieve the effects of high sensitivity, convenient collection, satisfactory precision and stability

Inactive Publication Date: 2016-03-23
SHANGHAI DIAN CLINICAL TESTING CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The collection of urine is less invasive than that of blood, and the content of cotinine in urine is...

Method used

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  • LC-MS/MS kit for detecting nicotine and its metabolites in saliva
  • LC-MS/MS kit for detecting nicotine and its metabolites in saliva
  • LC-MS/MS kit for detecting nicotine and its metabolites in saliva

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] 1. Materials

[0064] The samples of the methodological research experiment came from the saliva samples of the employees of Shanghai Dean Medical Laboratory.

[0065] (1) Instrument: API4000 + Triple quadrupole mass spectrometer (ABSCIEX company); ekspert'ultraLC100 liquid chromatography system (with automatic sampler); medical high-speed refrigerated centrifuge (Beijing Baiyang Medical Equipment Co., Ltd.); ultrapure water instrument (Chengdu Youpuchao) Pure Technology Co., Ltd.); multi-tube vortex mixer (Hangzhou Aosheng Instrument Co., Ltd.); fast mixer (Jiangyan Kangtai Medical Equipment Factory); adjustable pipette (ThermoScientific0.5~10μL, 10~100μL , 100~1000μL); glassware, beakers, measuring cylinders, etc.

[0066] (2) Reagent consumables: chromatographically pure methanol and acetonitrile (Tedia High Purity Solvent Company.Inc); Shanghai Yuexu Technology chromatographic column UltimateSiO2 (2.1×100mm, 3μm); 1.5mL centrifuge tube (AXYGEN, USA).

[0067] (3)...

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Abstract

The invention discloses an LC-MS/MS kit for detecting nicotine and its metabolites in saliva. The metabolites are cotinine and 3-hydroxyl cotinine. The kit includes the following reagents: two types of eluent, mixed standard liquid A, internal standard liquid B, two types of diluent, an organic solvent and quality control substance, wherein the eluent A is 0.1% v/v of a formic acid aqueous solution, the eluent B is 0.1% v/v of a formic acid methanol solution, the mixed standard liquid A is a methanol solution containing nicotine, cotinine and 3-hydroxyl cotinine, the internal standard liquid B is an internal standard solution containing deuterated cotinine, the dilute 1 is non-smoker's saliva, the diluent 2 is methanol, the organic solvent is acetonitrile, and the quality control substance is non-smoker's saliva containing nicotine, cotinine and 3-hydroxyl cotinine. The kit is high in detection sensitivity, good in specificity, accurate and simple in pretreatment process, saliva samples are convenient to collect, no wound is caused, and the kit can be used for evaluation of smoking and smoking cessation behaviors and research of environment smoke exposure degree.

Description

technical field [0001] The invention relates to a kit for detecting nicotine and its metabolites in saliva by LC-MS / MS, belonging to the technical field of analysis and detection. Background technique [0002] Nicotine is an important component in tobacco. Both active smoking and passive smoking will have adverse effects on the body, such as cardiovascular disease, chronic obstructive pulmonary disease and even cancer. Smoking addiction has become a prominent status quo that endangers public health in today's society. The main metabolites of nicotine in the human body include various substances such as cotinine and 3-hydroxy cotinine, among which cotinine accounts for 70%-80% of all metabolites. According to reports, the concentration levels of nicotine and its metabolites in different body fluids (blood, urine, and saliva) are closely related to smoke exposure, and their levels can evaluate smoking, smoking cessation behavior, and environmental smoke exposure. Therefore, i...

Claims

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Application Information

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IPC IPC(8): G01N30/88
CPCG01N30/88
Inventor 周亚飞于嘉屏
Owner SHANGHAI DIAN CLINICAL TESTING CENT
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