Preparation method of hydrogel nanoparticles capable of adsorbing melittin and detection method of affinity of hydrogel nanoparticles

Abstract

The invention discloses a preparation method of hydrogel nanoparticles capable of adsorbing melittin and a detection method of affinity of the hydrogel nanoparticles. The preparation method comprises the steps that N,N-diethylacrylamide, acrylic monomers, a cross-linking agent and an emulsifier are dissolved in a container through deionized water according to proportions, nitrogen is blown into the container at room temperature to remove oxygen, magnetic stirring is conducted, the reaction temperature is raised to 60-80 DEG C, heat preservation is conducted for 20 min-60 min, the nitrogen atmosphere is maintained, an initiating agent is added to trigger a polymerization reaction, dialysis is conducted, and a hydrogel nanoparticle solution is obtained. The detection method comprises the step that hemdytic effect studies are adopted to detect the affinity between the hydrogel nanoparticles and the melittin. According to the preparation method of the hydrogel nanoparticles capable of adsorbing the melittin and the detection method of the affinity of the hydrogel nanoparticles, synthesis processes are all completed in an aqueous phase, the used monomers and auxiliaries are all common reagents, green and environmentally friendly, and the methods are easy to achieve, so that the hydrogel nanoparticles (NPs) have the advantages of being low in price and easy to obtain. The prepared hydrogel nanoparticles have an obvious adsorption effect on the melittin.

Description

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine

Login to View More

AI Technical Summary

Benefits of technology

This patented method allows for efficient production of small molecules with high purity without generating harmful chemical waste or requiring expensive equipment such as solvents. These methods use commonly available materials like formaldehydes instead of complex organic compounds. Additionally, these techniques allow for precise control over how many different types of polymer chains form during reaction (molality) and how they interact together through interactions within each chain's structure.

Problems solved by technology

This patented technical problem addressed in this patents relating to making nano particles called plasmon hydrocilsensors (PNHC' s). These particles were found to attach themselves to other materials like metal surfaces when they interacted chemistryally. They could potentially replace existing drugs used in cancer treatment due to their ability to bind specifically to certain targets while being less harmful than traditional medications. Additionally, these particles had specific functions depending upon how well they work under physiological conditions.

Images