Compound extraction method of ganoderma triterpenes and triterpenoid saponin with high concentrations

A technology of Ganoderma lucidum triterpenes and triterpenoid saponins is applied in the directions of pharmaceutical formulations, plant raw materials, plant/algae/fungus/moss components, etc., can solve the problems of yield impact, Ganoderma lucidum is not pretreated, and achieves low production cost, Suitable for promotion, the effect of high concentration of bioactive ingredients

Inactive Publication Date: 2016-04-13
FUJIAN JIANSHEN BIOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The invention provides a method for compound extraction of high-concentration Ganoderma lucidum triterpenoids and triterpenoid saponins, which overcomes the supercritical CO 2 Extraction technology: Ganoderma lucidum was not pretreated when extracting triterpenoids from Ganoderma lucidum, and the fruiting body of Ganoderma lucidum was directly crushed and then supercritical CO was added. 2 The relevant parameters are controlled in the extraction kettle of the extraction equipment, and the fruiting body of Ganoderma lucidum is composed of cellulose, hemicellulose, lignin and other main components, and the structure is compact, so the simply crushed Ganoderma lucidum powder is directly subjected to CO 2 Supercritical extraction, the defect that its yield is affected, the present invention provides a kind of process is simple, the extraction method that can extract high-concentration ganoderma triterpenes and triterpene saponins that is suitable for popularization

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1: a kind of high-concentration Ganoderma lucidum triterpenes and triterpene saponin compound extraction method comprises the following steps:

[0014] (1) Crushing: Select top-grade Ganoderma lucidum, slice it, crush it and pass it through a 100-mesh sieve;

[0015] (2) Reduction treatment: Weigh 300g of Ganoderma lucidum powder obtained in step (1) and add it to 1200ml of 1% ascorbic acid solution for reduction treatment for 2 hours to obtain the reduced Ganoderma lucidum mixture;

[0016] (3) Freezing and thawing: place the reduced Ganoderma lucidum mixture processed in step (2) in a freeze-thaw box, add liquid nitrogen and freeze-thaw repeatedly 8 times;

[0017] (4) Wall breaking: Use 40% ethanol solution to ultrasonically break the wall of the reduced Ganoderma lucidum mixture that has been freeze-thawed in step (3). The ultrasonic output power is 440W, and the ultrasonic frequency is 50kHz. Ultrasonic treatment for 28 minutes, concentrated ultrasonic ...

Embodiment 2

[0020] Embodiment 2: a kind of high-concentration Ganoderma lucidum triterpenes and triterpene saponins compound extraction method, comprises the following steps:

[0021] (1) Crushing: Select top-grade Ganoderma lucidum, slice it, crush it and pass it through a 100-mesh sieve;

[0022] (2) Reduction treatment: Weigh 200g of Ganoderma lucidum powder obtained in step (1) and add it to 1000ml of 1% ascorbic acid solution for reduction treatment for 1 hour to obtain the reduced Ganoderma lucidum mixture;

[0023] (3) Freezing and thawing: place the reduced Ganoderma lucidum mixture processed in step (2) in a freeze-thaw box, add liquid nitrogen and freeze-thaw repeatedly 6 times;

[0024] (4) Breaking the wall: Use 40% ethanol solution to perform ultrasonic cavitation and breaking the wall of the reduced Ganoderma lucidum mixture that was freeze-thawed in step (3). The ultrasonic output power is 440W, and the ultrasonic frequency is 60kHz. Ultrasonic treatment for 32 minutes, co...

Embodiment 3

[0027] Embodiment 3: a kind of high-concentration Ganoderma lucidum triterpenes and triterpene saponins compound extraction method, comprises the following steps:

[0028] (1) Crushing: Select top-grade Ganoderma lucidum, slice it, crush it and pass it through a 100-mesh sieve;

[0029] (2) Reduction treatment: Weigh 400g of Ganoderma lucidum powder obtained in step (1) and add it to 1200ml of 1% ascorbic acid solution for reduction treatment for 1.5 hours to obtain the reduced Ganoderma lucidum mixture;

[0030] (3) Freezing and thawing: place the reduced Ganoderma lucidum mixture processed in step (2) in a freeze-thaw box, add liquid nitrogen and freeze-thaw repeatedly 7 times;

[0031] (4) Wall breaking: use 40% ethanol solution to ultrasonically break the wall of the reduced Ganoderma lucidum mixture that was freeze-thawed in step (3), with an ultrasonic output power of 420W and an ultrasonic frequency of 55kHz, ultrasonic treatment for 30 minutes, stagnation for 12 minute...

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Abstract

The invention discloses a compound extraction method of ganoderma triterpenes and triterpenoid saponin with high concentrations, relating to an extraction technology for active ingredients of lucid ganoderma. The compound extraction method comprises six steps of including smashing, reduction treatment, freeze thawing, wall breaking, supercritical CO2 extraction and freeze drying. According to the compound extraction method, before the supercritical CO2 extraction is carried out, lucid ganoderma powder is subjected to reduction, repetitive freeze-thawing and wall breaking treatment, ascorbic acid is adopted for carrying out antioxidation treatment on lucid ganoderma powder, so that the oxidation of ganoderma triterpenes and triterpenoid saponin substance is prevented, further, the compact structure of the sporocarp of lucid ganoderma is completely broken in combination with the repetitive freeze-thawing and ultrasonic wall breaking technologies, so that the bioactive components with high concentrations are guaranteed, and in addition, the industrial production cost is low, the implementation is convenient, and the market prospect is very hopeful.

Description

technical field [0001] The invention relates to a technology for extracting effective components of Ganoderma lucidum, in particular to a compound extraction method of high-concentration Ganoderma lucidum triterpenoids and triterpenoid saponins. Background technique [0002] Ganoderma lucidum, also known as Lin Zhongling and Qiongzhen, is umbrella-shaped in shape, with a kidney-shaped, semicircular or nearly round cap, and is the fruiting body of the fungus Ganoderma lucidum of Polyporaceae. It has the effects of invigorating qi and calming the nerves, relieving cough and relieving asthma, and is used for dizziness and insomnia, palpitation, shortness of breath, neurasthenia, cough and asthma due to fatigue, and prolongs life. Ganoderma lucidum has a medicinal history of thousands of years in China. Sun Simiao, the drug king, began to take Ganoderma lucidum all his life at the age of 35, and died at the age of 101. He has witnessed the longevity of Ganoderma lucidum througho...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): A61K36/074
CPCA61K36/074A61K2236/15A61K2236/37A61K2236/51
Inventor张英魏道智毛德春毛帅林文津宁书菊钱和
OwnerFUJIAN JIANSHEN BIOLOGICAL ENG