Living cell product preservation solution and preparation and using methods thereof
A technology for preserving liquid and living cells, applied in the field of cell culture, can solve the problems of not maintaining cell stability of cell metabolism and nutrients, unable to preserve living cells for a long time, and decreasing cell viability, so as to facilitate rapid and large-scale preparation and maintenance. The effect of stability, simple components
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Embodiment 1
[0048] A living cell product preservation solution, comprising the following components in the following mass percentage concentrations: 0.5% catechin, 1% glucose, 1% human serum albumin, 1% sodium bicarbonate; the balance is the preservation base solution , the preservation base liquid is composed of 0.9% sodium chloride injection and compound electrolyte injection according to the volume ratio of 1:1.
[0049] The catechins used in this example were prepared by the following method:
[0050] S01. Extraction: Weigh a certain amount of tea leaves, crush them through a 20 μm filter screen, add 15-25 times the weight of hot water at 70-90°C, stir and extract at a constant temperature for 20-60 minutes, and filter to obtain tea extracts;
[0051] S02, salting out: adding sodium chloride to the tea extract, so that the mass fraction of sodium chloride is 2-6%, let stand and salt out for 0.5-1.5h and then filter;
[0052] S03, Precipitation: Add 2-5% sodium bisulfite by weight of ...
Embodiment 2
[0064] The living cell product preservation solution contains the following components in the following mass percentage concentrations: 5% catechin, 10% glucose, 5% human serum albumin, and 10% sodium bicarbonate; the balance is the preservation base solution, The preservation base liquid is composed of 0.9% sodium chloride injection and compound electrolyte injection according to the volume ratio of 3:1.
[0065] In this example, the living cell product preservation solution is prepared by the following method: mix 0.9% sodium chloride solution and compound electrolyte solution at a volume ratio of 3:1 to obtain a preservation base solution; add catechin and glucose to the base preservation solution , human serum albumin, sodium bicarbonate, so that the mass percent concentration of catechin is 5%, the mass percent concentration of glucose is 10%, the mass percent concentration of human serum albumin is 5%, and the mass percent concentration of sodium bicarbonate The mass per...
Embodiment 3
[0068] The living cell product preservation solution contains the following components in the following mass percentage concentrations: 2% catechin, 5% glucose, 3% human albumin, and 5% sodium bicarbonate; the balance is the preservation base solution, The preservation base liquid is composed of 0.9% sodium chloride injection and compound electrolyte injection according to the volume ratio of 2:1.
[0069] The preparation and use methods of the living cell product preservation solution in this example are the same as those in Example 1.
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