Living cell product preservation solution and preparation and using methods thereof

A technology for preserving liquid and living cells, applied in the field of cell culture, can solve the problems of not maintaining cell stability of cell metabolism and nutrients, unable to preserve living cells for a long time, and decreasing cell viability, so as to facilitate rapid and large-scale preparation and maintenance. The effect of stability, simple components

Active Publication Date: 2016-04-20
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, when plan 1 simply uses physiological saline as the preservation solution, there are no nutrients to maintain cell metabolism and components to maintain cell stability, so living cells cannot be preserved for a long time, and the cell viability drops significantly after 24 hours

Method used

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  • Living cell product preservation solution and preparation and using methods thereof
  • Living cell product preservation solution and preparation and using methods thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] A living cell product preservation solution, comprising the following components in the following mass percentage concentrations: 0.5% catechin, 1% glucose, 1% human serum albumin, 1% sodium bicarbonate; the balance is the preservation base solution , the preservation base liquid is composed of 0.9% sodium chloride injection and compound electrolyte injection according to the volume ratio of 1:1.

[0049] The catechins used in this example were prepared by the following method:

[0050] S01. Extraction: Weigh a certain amount of tea leaves, crush them through a 20 μm filter screen, add 15-25 times the weight of hot water at 70-90°C, stir and extract at a constant temperature for 20-60 minutes, and filter to obtain tea extracts;

[0051] S02, salting out: adding sodium chloride to the tea extract, so that the mass fraction of sodium chloride is 2-6%, let stand and salt out for 0.5-1.5h and then filter;

[0052] S03, Precipitation: Add 2-5% sodium bisulfite by weight of ...

Embodiment 2

[0064] The living cell product preservation solution contains the following components in the following mass percentage concentrations: 5% catechin, 10% glucose, 5% human serum albumin, and 10% sodium bicarbonate; the balance is the preservation base solution, The preservation base liquid is composed of 0.9% sodium chloride injection and compound electrolyte injection according to the volume ratio of 3:1.

[0065] In this example, the living cell product preservation solution is prepared by the following method: mix 0.9% sodium chloride solution and compound electrolyte solution at a volume ratio of 3:1 to obtain a preservation base solution; add catechin and glucose to the base preservation solution , human serum albumin, sodium bicarbonate, so that the mass percent concentration of catechin is 5%, the mass percent concentration of glucose is 10%, the mass percent concentration of human serum albumin is 5%, and the mass percent concentration of sodium bicarbonate The mass per...

Embodiment 3

[0068] The living cell product preservation solution contains the following components in the following mass percentage concentrations: 2% catechin, 5% glucose, 3% human albumin, and 5% sodium bicarbonate; the balance is the preservation base solution, The preservation base liquid is composed of 0.9% sodium chloride injection and compound electrolyte injection according to the volume ratio of 2:1.

[0069] The preparation and use methods of the living cell product preservation solution in this example are the same as those in Example 1.

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Abstract

The invention relates to a living cell product preservation solution and preparation and using methods thereof. The living cell product preservation solution is prepared from catechinic acid, glucose, human serum albumin, sodium bicarbonate and preservation base fluid. The preservation base fluid is composed of a 0.9% sodium chloride solution and a compound electrolyte solution. The catechinic acid, the glucose, the human serum albumin and the sodium bicarbonate are added into the preservation base fluid, a 0.22-micrometer filter film is used for filtration sterilization, and the living cell product preservation solution is obtained. Living cell product preservation solution resuspension cells are used, the cell concentration is 0.5*10<7>-5*10<7> cells/ml, and normal temperature preservation is carried out for 0-72 h. The living cell product preservation solution can be used for preserving living cells for a long time, and living cells still have high activity and motility rate after being preserved through the preservation solution for 72 h. The living cell product preservation solution can preserve living cells at normal temperature.

Description

technical field [0001] The invention relates to the field of cell culture, in particular to a living cell product preservation solution and its preparation and use methods. Background technique [0002] Living cell products generally refer to living cells that are induced and cultured in vitro or directly collected from the body and have certain therapeutic and health care effects. At present, the most common living cell products are stem cells and immune cells, as well as human living cells such as nerve cells and liver cells. [0003] With the development of the function of stem cells and immune cells and the research of disease treatment, especially the highlight of the therapeutic effect of immune cells on tumor diseases, the clinical application of living cells such as stem cells or immune cells is also becoming more and more extensive. The preservation of living cells such as stem cells and immune cells before reinfusion is very important, especially when it comes to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 葛啸虎陈海佳王一飞应杰王小燕
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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