Tetradium ruticarpum fruit effective ingredient extract as well as preparation method and application thereof

A technology of active ingredients and extracts, which is applied in the preparation of evodius alkaloids and the application field of medicine for treating migraine, can solve the problems of backwardness of evodius, different components and related medicinal effects, etc.

Inactive Publication Date: 2016-04-20
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the extraction method of Evodia rutaecarpa is relatively backwar

Method used

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  • Tetradium ruticarpum fruit effective ingredient extract as well as preparation method and application thereof
  • Tetradium ruticarpum fruit effective ingredient extract as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: the preparation of Evodia rutaecarpa extract

[0017] Take 9kg of Evodia rutaecarpa, crush it, pass through a 20-mesh sieve, add 6 times the amount of 95% ethanol and soak overnight. Reflux extraction 3 times, 1 hour each time, the extract was filtered through 6 layers of gauze while it was hot, the filtrates were combined for 3 times, suction filtered with 1 layer of filter paper, concentrated under reduced pressure (below 60%) and recovered ethanol to obtain 1800 g of dry paste. Add 10L of boiling water and knead to dissolve, divide into the supernatant and the remaining part after knead and dissolve. Take the supernatant and pass through the HPD-100 macroporous resin column, elute with 10%, 30%, 50%, and 95% ethanol (each 4 times the column volume) respectively, reclaim the ethanol below 60%, and obtain the dry paste of each part as follows: 86, 148, 145, 13g. After TLC detection, the 30%, 50% ethanol eluted fractions were combined. Put it in a dialy...

Embodiment 2

[0018] Example 2: Preparation of Evodia Evodia Extract

[0019] Take 90 grams of Evodia rutaecarpa in 30 times the amount of water at 70°C, stir slowly for 15 minutes, filter out with 4 layers of gauze, discard the filtrate, repeat the operation 7 times, dry the final obtained Evodia rutaecarpa at 60°C, add ten times the amount of water, and soak 30min, decoct for 1h, filter the decoction with 4 layers of gauze, repeat the decoction once, combine the filtrate, concentrate under low pressure to about 1.5 liters, freeze-dry the concentrated solution into powder, the powder weight is about 14.0 grams, and the powder yield is 15.6% . Take 0.02g of lyophilized powder, add 1ml of methanol precisely, ultrasonicate for 30min, centrifuge at 15000rpm for 3min, and take the supernatant. Put it in a dialysis bag with a pore size of 500D, cover it with a dialysis bag with a pore size of 100D, and tie the mouth. Soak it in a beaker of double distilled water and let it stand at 4°C for 48h...

Embodiment 3

[0020] Example 3: Pharmacological Experiment of Evodia Evodia Extract

[0021] The mice were randomly divided into 8 groups, 12 in each group (half male and half female), each subcutaneously injected with reserpine 10 mg / kg for 14 days. On the 14th day, the mice were anesthetized with ether, and at the same time, each mouse was orally administered the positive control products sibeline, zhennaoning and the ingredients <100D and 100D-500D extracted from Evodia rutaecarpa for 3 consecutive days. Pain threshold was measured by hot plate method 1 hour after the last administration.

[0022]

[0023] Summary of Results

[0024] It can be concluded from Example 3 that the 100D-500D Evodia rutaecarpa extract significantly improves the pain threshold of mice, so it is speculated that it has better biological activity and may have better curative effect on migraine.

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Abstract

The invention provides a tetradium ruticarpum fruit effective ingredient extract. The extract comprises evodiamine, isorhamnetin and limonin and has the molecular weight of 100-500D. The invention further provides a preparation method of the tetradium ruticarpum fruit effective ingredient extract. The method has the characteristics of low cost, facilitation of industrial production, simplicity, convenience and high efficiency.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine pharmacy, and in particular relates to a preparation method of evodia rutaecarpa alkaloids and the application of active substances in medicines for treating migraine. Modern production technology and pharmacoeconomics are used to realize its low-cost and easy-to-industrial preparation process, so as to ensure the lowest cost and the greatest benefit to meet the demand for effective substances in clinical treatment. Background technique [0002] Evodia rutaecarpa is a commonly used traditional Chinese medicine, which was first recorded in "Shen Nong's Materia Medica" and "Chinese Pharmacopoeia" 2005 edition. It has the functions of dispelling cold and relieving pain, lowering adverse flow and relieving vomiting, supporting yang and relieving diarrhea. Evodia rutaecarpa contains many types of chemical components, including alkaloids, bitters, terpenes, flavonoids, coumarin, steroids, vo...

Claims

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Application Information

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IPC IPC(8): A61K36/754A61K31/519A61K31/352A61K31/585A61P25/06A61P29/02
CPCA61K31/352A61K31/519A61K31/585A61K36/754A61K2236/331A61K2236/39A61K2236/51A61K2236/53A61K2236/55A61K2300/00
Inventor 胡紫霞毕开顺李清许华容袁子越
Owner SHENYANG PHARMA UNIVERSITY
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