A kind of yellow fluorescent carbon dot with high quantum yield and preparation method thereof

A technology of yellow fluorescence and quantum yield, which is applied in the field of yellow fluorescent carbon dots and its preparation, can solve the problems of limited application, reduced quantum yield, and poor penetration, and achieve simple and fast process, stable optical properties, and quantum yield. high effect

Active Publication Date: 2017-12-01
LANZHOU UNIVERSITY
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, there are still two key problems in the research of carbon dots: first, the quantum yield of fluorescent carbon dots is low and needs to be improved urgently; second, most fluorescent carbon dots emit blue fluorescence, and the emission wavelength is longer There are few reports of fluorescent carbon dots
Since the autofluorescence of biological tissues is mostly blue, blue fluorescent carbon dots are not conducive to the distinction between target signals and background signals when applied to biological imaging; in addition, blue light has poor penetration into tissues, which limits the optical properties of deep tissues in vivo. imaging
Although some excitation wavelength-dependent fluorescent carbon dots can adjust the emission wavelength by adjusting the excitation wavelength, the quantum yield of non-blue light emission decreases rapidly, which limits its application in biology.

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  • A kind of yellow fluorescent carbon dot with high quantum yield and preparation method thereof
  • A kind of yellow fluorescent carbon dot with high quantum yield and preparation method thereof
  • A kind of yellow fluorescent carbon dot with high quantum yield and preparation method thereof

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Embodiment 1

[0029] At room temperature, dissolve 0.05 g of malic acid and 0.2 g of mercaptoethylamine in 5 ml of water, mix them well, put them in a microwave oven, and irradiate them at a power of 800 W for 3 min to obtain a brown solid crude product. After natural cooling at room temperature, water and Rinse with methanol mixed solution, and purify by reverse phase silica gel column chromatography to obtain yellow fluorescent carbon dot material. Its excitation wavelength is 405 nanometers, its emission wavelength is 510 nanometers, and exhibits solid-state luminescent properties. The quantum yield of yellow fluorescent carbon dots can reach 38%.

Embodiment 2

[0031] At room temperature, dissolve 0.2 g of ethylenediamine tetraacetic acid and 0.7 g of aminoethanol in 5 ml of water, mix well, place in a microwave oven, and irradiate at a power of 700 W for 5 min to obtain a brown solid crude product. After natural cooling at room temperature Rinse with a mixed solution of water and methanol, and purify by reverse-phase silica gel column chromatography to obtain a yellow fluorescent carbon dot material. Its excitation wavelength is 410 nanometers, its emission wavelength is 520 nanometers, and exhibits solid-state luminescent properties. The quantum yield of yellow fluorescent carbon dots can reach 41%.

Embodiment 3

[0033] At room temperature, dissolve 0.3 g of trimesic acid and 0.2 g of ethylenediamine in 5 ml of water, mix well, place in a microwave oven, and irradiate at a power of 600 W for 7 min to obtain a brown solid crude product. After natural cooling at room temperature Rinse with a mixed solution of water and methanol, and purify by reverse-phase silica gel column chromatography to obtain a yellow fluorescent carbon dot material. Its excitation wavelength is 415 nanometers, its emission wavelength is 525 nanometers, and exhibits solid-state luminescent properties. The quantum yield of yellow fluorescent carbon dots can reach 44%.

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Abstract

The invention relates to yellow fluorescence carbon dots with high quantum yield. A preparation method of the yellow fluorescence carbon dots is characterized by comprising the following steps: dissolving organic acid and organic amine in water; performing microwave radiation after fully mixing the organic acid and the organic amine, thus obtaining a brown solid crude product; performing reverse-phase silica gel column chromatography purification by taking a mixed solution of the water and methyl alcohol as an eluting agent, thus obtaining the yellow fluorescence carbon dots. The yellow fluorescence carbon dots are characterized in that the average grain diameter is 3.0 to 4.0 nm; bright yellow fluorescent light can be emitted under the irradiation of an ultraviolet lamp, the excitation wavelength is 400 to 420 nm, the emission wavelength is 510 to 550 nm, the luminescent property of solids is shown, and the quantum yield can reach 44 percent. The yellow fluorescence carbon dots prepared by the invention are good in water solubility and stable in fluorescence signals, are suitable for cell imaging under physiological conditions, have a function of nuclear staining and can be widely applied to the fields of analysis and detection, biochemical sensing, biological imaging, biological marking and the like.

Description

technical field [0001] The invention relates to a fluorescent carbon nanometer material, in particular to a yellow fluorescent carbon point with high quantum yield and its preparation, which is mainly used for biological imaging and biological labeling under physiological conditions. Background technique [0002] As a new member of the carbon material family, carbon dots are one of the most popular carbon nanomaterials after fullerenes, carbon nanotubes, and graphene. Carbon dots are usually composed of four basic elements, C, H, O, and N. The particle size is generally less than 10 nanometers, with good optical properties, adjustable luminous range, and good photostability. Compared with traditional organic dyes and semiconductor quantum dots, fluorescent carbon dots have the advantages of good water solubility and chemical stability, easy functionalization, low cytotoxicity and excellent biocompatibility. research interests, and has shown great application potential in th...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C09K11/65B82Y20/00
CPCB82Y20/00C09K11/65
Inventor周雷赵凤姣
OwnerLANZHOU UNIVERSITY