A kind of separation method of air-dried fruiting bodies of white ginseng fungus
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A technology for the separation of white ginseng fungus and bacterial species, applied in the field of microorganisms, can solve the problems of inability to cultivate mycelium, incapable of bacterial species, separation, etc., to achieve the improvement of production level and economic benefit, the neatness of mushrooms, and the improvement of biological efficiency. Effect
Inactive Publication Date: 2019-08-09
WENSHAN UNIV
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Problems solved by technology
The inventor referred to the methods of the above two studies, and the hyphae could not be cultured at all, let alone the separation of the strains
Method used
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Examples
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Embodiment 1
[0031] 1) Soak naturally air-dried white ginseng fungus fruiting bodies in sterile water for 3 hours.
[0032] 2) Then put the soaked white ginseng fruiting bodies into the tissue culture bottle, wet the gauze with sterile water to cover the bottle mouth, put it into an artificial climate incubator for shading culture, keep the humidity at 80%-85%, The temperature is set at 12°C, and after 7-10 days, white dense hyphae can grow at the base of the stipe of the white ginseng fungus.
[0033] 3) Cut off the white ginseng fungus stipe base with a length of 0.5cm to 1.0cm with white mycelium, then move it into the clean bench, use a long and thin needle or iron wire to gently pick the basic white mycelium of the stipe and insert it into the preparation A good medium (20g of potatoes per liter, 2.5g of peptone, 2.5g of soybean protein, 20g of sugar, 0.5g of magnesium sulfate, 1g of potassium dihydrogen phosphate, 20g of agar, pH 5.6-5.8), and then placed in artificial climate In th...
Embodiment 2
[0042] Except that the fruiting bodies were air-dried and stored at 4° C. for one year, the others were consistent with Example 1. The results obtained are: the strains were successfully isolated, and the biological efficiency was 66.3% and 50.2% when the fruiting experiment was carried out. Compared with the control group, the fruiting was neat and the flowers were large.
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Abstract
The invention provides a method for isolating strains of air-dried fruiting bodies of schizophyllum commune. The method includes steps of 1), soaking and fattening the naturally air-dried fruiting bodies of the schizophyllum commune by the aid of sterile water; 2), cultivating the fruiting bodies obtained in the step 1) at the temperature of 12 DEG C for 7-10 days and fetching white hyphae grown on roots of stipe; 3), cultivating the hyphae obtained in the step 2) in cultivation media to obtain colonies; 4), purifying and then rejuvenating the colonies obtained in the step 3) to obtain the strains. The method has the advantages that shortcomings of constraints due to seasonal factors, high storage cost for fresh fruiting bodies, easiness in contamination and the like when strains are about to be acquired from the fresh fruiting bodies of schizophyllum commune can be overcome by the aid of the method, and the strains can be isolated and cultivated to produce schizophyllum commune at any time all through the year; the schizophyllum commune production biological efficiency can be greatly improved and can be improved by 35%; the obtained schizophyllum commune is neat and has large sizes when the strains are cultivated for producing the schizophyllum commune.
Description
technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a method for separating air-dried fruiting bodies of white ginseng fungi. Background technique [0002] White ginseng is an alias of Schizophyllum, in Heilongjiang, Jilin, Liaoning, Shanxi, Shandong, Jiangsu, Inner Mongolia, Anhui, Zhejiang, Jiangxi, Fujian, Taiwan, Hebei, Henan, Hunan, Guangdong, Guangxi, Hainan, Gansu, It is distributed in Tibet, Sichuan, Guizhou and Hainan. People in Yunnan Province have a long history of eating white ginseng fungus, and the products are exported. [0003] With the continuous improvement of living standards, people's demand for white ginseng bacteria is increasing, which makes how to increase the output of white ginseng bacteria an important research topic. In this research topic, how to solve the problem that the production of white ginseng fungus is severely restricted by seasons is one of the important research directions. ...
Claims
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