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Application of chlorogenic acid and/or chlorogenic acid derivative in tumor stem cell treatment drug

A technology of tumor stem cells and anti-tumor drugs, applied in anti-tumor drugs, drug combinations, drug delivery, etc., can solve problems such as the ineffectiveness of tumor stem cells, inability to eliminate tumor stem cells, tumor recurrence and metastasis, etc., to achieve weakening of self-renewal ability, Effect of inhibiting proliferation and preventing recurrence

Inactive Publication Date: 2016-05-18
SICHUAN JIUZHANG BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although chemotherapy and radiotherapy can kill most tumor cells, it has no effect on tumor stem cells. After proliferation of tumor stem cells, it can eventually lead to tumor recurrence
Therefore, chemotherapy and radiotherapy cannot eliminate tumor stem cells in the body, and as long as there are tumor stem cells in the body, the tumor may relapse and metastasize

Method used

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  • Application of chlorogenic acid and/or chlorogenic acid derivative in tumor stem cell treatment drug
  • Application of chlorogenic acid and/or chlorogenic acid derivative in tumor stem cell treatment drug
  • Application of chlorogenic acid and/or chlorogenic acid derivative in tumor stem cell treatment drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Effects of chlorogenic acid on the growth of lung cancer stem cells

[0032] Tumor sphere formation experiment: The cells of conventionally cultured human lung cancer cell lines H460 and H466 were digested with trypsin and centrifuged at a centrifugal speed of 1000rpm for 2min, discarded the supernatant, added 3ml of sterile phosphate buffered saline (PBS), and blown gently , Wash the cell pellet and centrifuge. After repeated washing for 3 times, tumorsphere medium (serum-free medium) was added to prepare a single cell suspension.

[0033] Measure the cell concentration of the prepared single-cell suspension of cell line H460 and adjust the cell density to 1500cells / ml, transfer the single-cell suspension evenly to an ultra-low adhesion 6-well plate, and set up A, B, and C respectively. Tumorsphere culture was carried out with group D; the cell concentration of the prepared single cell suspension of cell line H466 was measured and the cell density was adjusted to 1500...

Embodiment 2

[0039] Effects of chlorogenic acid on breast cancer stem cells

[0040] 1. Serum-free Suspension Microsphere Culture of Mouse Breast Cancer Cell Line MCF-7—Enrichment of Breast Cancer Stem Cells

[0041]The purchased MCF-7 breast cancer cells were routinely revived, cultured in DMEM / F12 medium (SSM) containing 10% fetal bovine serum, and digested with 0.25% trypsin-0.02% EDTA for passage. MCF-7 cells in the logarithmic growth phase cultured in SSM were digested with 0.25% trypsin-0.02% EDTA and mechanically blown into a single cell suspension, stained with trypan blue and counted, inoculated at 2×104 / ml in Serum-free medium (SFM, that is, DMEM / F12 medium without fetal bovine serum, supplemented with 20ng / mlEGF, 20ng / mlbFGF, 2%B27), cultured statically in a 37°C, 5%CO2 incubator, centrifuged for 3 days Change the medium, and when the cell spheres are formed, digest them with a 10-fold dilution of 0.25% trypsin-0.02% EDTA to form a single-cell suspension, resuspend them in SFM,...

Embodiment 3

[0057] Effects of Chlorogenic Acid on Leukemia Stem Cells

[0058] Materials: acute myeloid leukemia stem cells or peripheral blood or bone marrow cells of patients with chronic myeloid leukemia in blast phase.

[0059] Methods: Peripheral blood cells were treated with leukocyte separation medium to remove red blood cells, bone marrow cells were treated with ammonium chloride and sodium bicarbonate to remove red blood cells, then cultured in serum-free medium and washed three times. They were divided into three groups, and treated with normal saline, chlorogenic acid at concentrations of 1 μg / mL and 5 μg / mL for 3 hours, washed with serum-free medium to remove chlorogenic acid, and treated with methylcellulose IMAM medium (1 % methylcellulose, 30% bovine serum albumin, 0.2mM mercaptoethanol, 2mM glutamine, 50ng / ml recombinant human stem cell factor, 10ng / ml erythropoietin, 50ng / ml granulocyte colony-producing factor) into 50000cells / ml, put into the incubator, cultivate for 10...

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Abstract

The invention provides an application of chlorogenic acid and / or a chlorogenic acid derivative in a tumor stem cell treatment drug and belongs to the technical field of medicine. According to the application of the chlorogenic acid and / or the chlorogenic acid derivative in the tumor stem cell treatment drug, the new application of the chlorogenic acid is provided, and the chlorogenic acid is applied to the tumor stem cell treatment drug, so that the tumor stem cells can be inhibited and killed by the drug and relapse, metastasis and multidrug resistance of tumors are effectively prevented.

Description

technical field [0001] The present invention relates to the technical field of medicine, in particular to the application of chlorogenic acid and / or its derivatives in medicine for treating tumor stem cells. Background technique [0002] 90% of cancer-related deaths are due to tumor recurrence and metastasis to other key organs. Tumor recurrence and metastasis are due to the existence of tumor stem cells. The American Association for Cancer Research (American Association for Cancer Research) gave a clear definition of cancer stem cells in 2006: cells in tumors that have self-renewal ability and can produce heterogeneous tumor cells. Cancer stem cells have the characteristics of unlimited self-renewal ability, multi-directional differentiation potential, high telomerase activity, strong drug resistance, anti-apoptosis, low proportion in tumor tissue and expression of stem cell-specific markers. Although chemotherapy and radiotherapy can kill most tumor cells, it has no effe...

Claims

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Application Information

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IPC IPC(8): A61K31/216A61P35/00
CPCA61K31/216A61K9/0019A61K9/145A61K9/2059
Inventor 张洁朱丽娜
Owner SICHUAN JIUZHANG BIO TECH CO LTD
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