Method for extracting ginsenoside Rg5
A technology of ginsenosides and ginseng powder, applied in chemical instruments and methods, glycoside steroids, steroids, etc., to achieve the effects of mild experimental conditions, high extraction yield and short extraction time
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Embodiment 1
[0027] A kind of extracting method of ginsenoside Rg5: get ginseng root, after it is pulverized, pass through 120 mesh sieves, get 0.5g ginseng powder, add the mixed solvent of n-butanol and water of 60 times of volume (the volume ratio of the two is 4:1) , adding hydrochloric acid to make the acid concentration 0.2mol / L, stirring and extracting in a 60°C water bath for 4h. The extract was centrifuged to separate the extract and the solid, and the solid was washed twice with saturated n-butanol, combined with the extract, and washed with saturated Na 2 CO 3 After the aqueous solution is neutralized, it is concentrated to dryness with a rotary evaporator at a temperature lower than 60° C. to obtain an extract containing ginsenoside Rg5.
Embodiment 2
[0029] A kind of extracting method of ginsenoside Rg5: get ginseng root, cross 100 mesh sieves after it is pulverized, get 0.5g ginseng powder, add the mixed solvent of methyl alcohol, n-butanol, water of 20 times of volumes (three volume ratios are 3 :5:2), add sulfuric acid to make the acid concentration 0.1mol / L, stir and extract in 80°C water bath for 2h. The extract was centrifuged to separate the extract and the solid, and the solid was washed twice with saturated n-butanol, combined with the extract, and washed with saturated Na 2 CO 3 After the aqueous solution is neutralized, it is concentrated to dryness with a rotary evaporator at a temperature lower than 60° C. to obtain an extract containing ginsenoside Rg5.
Embodiment 3
[0031]A kind of extracting method of ginsenoside Rg5: get ginseng root, after it is pulverized, pass through 120 mesh sieves, get 0.5g ginseng powder, add the mixed solvent (5:3:2) of the ethanol of 15 times volume, n-butanol, water , adding hydrochloric acid to make the acid concentration 0.06mol / L, stirring and extracting in a 70°C water bath for 6h. The extract was centrifuged to separate the extract and the solid, and the solid was washed twice with saturated n-butanol, combined with the extract, and washed with saturated Na 2 CO 3 After the aqueous solution is neutralized, it is concentrated to dryness with a rotary evaporator at a temperature lower than 60° C. to obtain an extract containing ginsenoside Rg5.
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