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New uses of caspase recruitment domain protein 9

A technology of use and protein expression, applied in the new application field of caspase recruitment domain protein 9, which can solve the problems of low sensitivity and incomplete elucidation of the pathogenesis of acute pancreatitis, and achieve the effect of high sensitivity

Inactive Publication Date: 2018-10-09
上海市松江区中心医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Ranson scoring system has played a great role in the diagnosis and treatment of severe pancreatitis, but compared with CT and other imaging examinations, it is found that its sensitivity is not high
[0004] To date, the exact pathogenesis of the initiation and progression of acute pancreatitis has not been fully elucidated

Method used

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  • New uses of caspase recruitment domain protein 9
  • New uses of caspase recruitment domain protein 9
  • New uses of caspase recruitment domain protein 9

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The preparation of embodiment 1 kit

[0047] Assemble the kit from the following reagents:

[0048] 1) Specific primer pair for CARD9:

[0049] Upstream primer: 5'-GCAGGTGTTCCAGTGTGAG-3'

[0050] Downstream primer 5'-GGCAGCCTTTGTCTGAGAG-3'

[0051] 2) Internal reference primer pair:

[0052] Upstream primer 5'-AATCCCATCACCATCTTC-3'

[0053] Downstream primer 5'-AGGCTGTTGTCATACTTC-3'

[0054] 3) Negative control: DEPC deionized water

[0055] 4) Positive control: construction of CARD9 gene plasmid DNA

[0056] 4.1 Primer design:

[0057]The upstream and downstream sequences of the target gene were queried from GenBank, and the primers were designed with Vector NTI software.

[0058] The primer sequences are:

[0059] Upstream primer: 5'-GCAGGTGTTCCAGTGTGAG-3'

[0060] Downstream primer 5'-GGCAGCCTTTGTCTGAGAG-3'

[0061] Template source: normal human peripheral blood mononuclear cells

[0062] PCR amplification target fragment:

[0063] Use the high-fidelity ...

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Abstract

The present invention relates to new uses of caspase recruitment domain-containing protein 9, further discloses uses of caspase recruitment domain-containing protein 9 as a biological biomarker in preparation of an acute pancreatitis severity diagnosis kit, and an acute pancreatitis severity diagnosis kit. According to the present invention, the caspase recruitment domain-containing protein 9 is adopted as the index to diagnose the acute pancreatitis severity, and has higher sensitivity compared to the RANSON scoring.

Description

technical field [0001] The invention relates to the fields of biotechnology and medicine, in particular to a new application of caspase recruiting domain protein 9 (CARD9). Background technique [0002] Acute pancreatitis (AP) is one of the common acute abdomen, often accompanied by systemic inflammatory response syndrome and multiple organ dysfunction syndrome, which seriously endangers the life of patients. Therefore, early diagnosis and prediction of the severity of pancreatitis have very important clinical significance. [0003] In the early 1970s, Ranson proposed the Ranson scoring system after studying 100 acute pancreatitis patients admitted for 48 hours. The scoring system includes 5 clinical indicators at admission and 1 point for each of the 6 indicators within 48 hours, totaling 11 points. Severe pancreatitis is defined as a score of 3 or more. The fatality rate is 0.9% for scores below 3, 16% for scores 3-4, 40% for scores 5-6, and 100% for scores above 6. The...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883G01N33/68G01N33/577
Inventor 徐萍杨志文王静翁成钊孟潇潇
Owner 上海市松江区中心医院