Lactobacillus rhamnosus preparation and application thereof

A technology of Lactobacillus rhamnosus and probiotics, applied in the field of biopharmaceuticals, can solve the problems of not being able to really change the allergic immune response, treating the symptoms but not the root cause

Active Publication Date: 2016-06-01
QINGDAO EASTSEA PHARMA
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, these drugs only provide temporary relief of symptoms and cannot really

Method used

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  • Lactobacillus rhamnosus preparation and application thereof

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preparation example Construction

[0019] Preparation Example The preparation of Lactobacillus rhamnosus powder

[0020] 1 Preparation of bacteria powder and identification of strains

[0021] Collect 5-10g of naturally discharged fresh feces in a sterile container, quickly put them into an anaerobic tank, and bring them back to the laboratory for strain isolation. Take 1.5g of feces sample, suspend and mix with 15ml PBS buffer, centrifuge at 400g for 5min to collect the supernatant, then add appropriate amount of PBS buffer and centrifuge at 8000g for 10min, collect the supernatant to obtain the test sample. Take 1ml of the supernatant solution and add it to a test tube containing 9ml of PBS diluent (containing 5‰ L-cysteine), vortex and mix well, as 10 -2 Diluent, dilute to 10 in the same way -3 , respectively take feces stock solution, 10 -2 Diluent, 10 -3 Spread 0.1ml of each dilution on the MRS plate, and place it in a constant temperature incubator at 37°C for 24 hours. After culturing for 24 hours, t...

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Abstract

The invention belongs to the field of biological medicine and relates to a lactobacillus rhamnosus preparation and application thereof, in particular to a method for preparing a microecological preparation from lactobacillus rhamnosus serving as a main active ingredient and application of the microecological preparation in treating allergy and other related diseases. The lactobacillus rhamnosus is lactobacillus rhamnosus DH099 with the preservation number of CGMCC No.11229.

Description

technical field [0001] The invention relates to a preparation of Lactobacillus rhamnosus and an application thereof, in particular to a microecological preparation made of Lactobacillus rhamnosus as a main active ingredient, and the application of the microecological preparation in treating related diseases, belonging to the field of biological medicine. Background technique [0002] Lactobacillus rhamnosus plays an increasingly important role in the prevention and treatment of diseases, but the products containing Lactobacillus rhamnosus that are currently on the market are not ideal, so screening high-efficiency Lactobacillus rhamnosus strains is an urgent need The problem to be solved, especially the screening of Lactobacillus rhamnosus strains with significant anti-allergic effect. [0003] In recent years, the incidence of allergic diseases has been on the rise. According to the data of the World Health Organization, 22% of the world's people suffer from allergic diseas...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/747A61P37/08A23L33/135A23K10/18C12R1/225
CPCA61K35/747C12N1/20
Inventor 崔云龙郁聪
Owner QINGDAO EASTSEA PHARMA
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