Lactobacillus acidophilus preparation and application thereof

A technology of Lactobacillus acidophilus and probiotics, applied in the field of biopharmaceuticals, can solve the problems of not being able to truly change allergic immune responses, treating the symptoms but not the root cause, etc.

Active Publication Date: 2016-05-18
QINGDAO EASTSEA PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, these drugs only provide temporary relief of symptoms and cannot really change the allergic immune response, treating the symptoms but not the root cause

Method used

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  • Lactobacillus acidophilus preparation and application thereof

Examples

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preparation example Construction

[0019] Preparation Example The preparation of Lactobacillus acidophilus powder

[0020] 1 Preparation of bacteria powder and identification of strains

[0021] Collect 5-10g of naturally discharged fresh feces in a sterile container, quickly put them into an anaerobic tank, and bring them back to the laboratory for strain isolation. Take 1.5g of feces sample, suspend and mix with 15ml PBS buffer, centrifuge at 400g for 5min to collect the supernatant, then add appropriate amount of PBS buffer and centrifuge at 8000g for 10min, collect the supernatant to obtain the test sample. Take 1ml of the supernatant solution and add it to a test tube containing 9ml of PBS diluent (containing 5‰ L-cysteine), vortex and mix well, as 10 -2 Diluent, dilute to 10 in the same way -3 , respectively take feces stock solution, 10 -2 Diluent, 10 -3 Spread 0.1ml of each dilution solution on the MRS plate, place it in a constant temperature incubator at 37°C for 24 hours, and select the colonies ...

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Abstract

The invention relates to a lactobacillus acidophilus preparation and application thereof, in particular to a micro-ecological preparation using lactobacillus acidophilus as a main active ingredient and application of the micro-ecological preparation in treatment of irritability and other related diseases and belongs to the field of biological medicines. The lactobacillus acidophilus is lactobacillus acidophilus DH213, and the preservation number is CGMCC No.11228.

Description

technical field [0001] The invention relates to a Lactobacillus acidophilus preparation and its application, in particular to a microecological preparation made of Lactobacillus acidophilus as the main active ingredient, and the application of the microecological preparation in treating related diseases, belonging to the field of biological medicine. Background technique [0002] Lactobacillus acidophilus plays an increasingly important role in the prevention and treatment of diseases, but the products containing Lactobacillus acidophilus that are currently on the market are not effective, so screening high-efficiency Lactobacillus acidophilus strains is an urgent problem to be solved at present , especially Lactobacillus acidophilus strains with significant anti-allergic effects were screened. [0003] In recent years, the incidence of allergic diseases has been on the rise. According to the data of the World Health Organization, 22% of the world's people suffer from allerg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P37/08A61P17/00A61P11/02A61P11/06A23K10/18A23L33/135C12R1/23
CPCC12N1/20A61K2035/11A23V2400/113
Inventor 崔云龙郁聪
Owner QINGDAO EASTSEA PHARMA
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