Lactobacillus paracasei preparation and its application

A technology of para-cheese and Lactobacillus, applied in the field of biopharmaceuticals, can solve the problems of no research reports, the inability to really change the allergic immune response, and treating the symptoms but not the root cause

Active Publication Date: 2019-08-30
QINGDAO EASTSEA PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these drugs only provide temporary relief of symptoms and cannot really change the allergic immune response, treating the symptoms but not the root cause
[0005] The microecological preparation of Lactobacillus paracasei of the present invention has remarkable curative effect in preventing and treating diseases such as allergies, and the effect is significantly better than that of other Lactobacillus paracasei, and can be used as medicine, health care product, food, veterinary drug and feed additive, etc., which has great social significance and has never been seen Relevant research reports, apply for this invention patent

Method used

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  • Lactobacillus paracasei preparation and its application

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preparation example Construction

[0019] Preparation Example The preparation of Lactobacillus paracasei powder

[0020] 1 Preparation of bacteria powder and identification of strains

[0021] Collect 5-10g of naturally discharged fresh feces in a sterile container, quickly put them into an anaerobic tank, and bring them back to the laboratory for strain isolation. Take 1.5g of feces sample, suspend and mix with 15ml PBS buffer, centrifuge at 400g for 5min to collect the supernatant, then add appropriate amount of PBS buffer and centrifuge at 8000g for 10min, collect the supernatant to obtain the test sample. Take 1ml of the supernatant solution and add it to a test tube containing 9ml of PBS diluent (containing 5‰ L-cysteine), vortex and mix well, as 10 -2 Diluent, dilute to 10 in the same way -3 , respectively take feces stock solution, 10 -2 Diluent, 10 -3 Spread 0.1ml of each dilution on the MRS plate, and place it in a constant temperature incubator at 37°C for 24 hours. After culturing for 24 hours, t...

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Abstract

The invention relates to a preparation of Lactobacillus paracasei and its application, in particular to a microecological preparation prepared from Lactobacillus paracasei as the main active ingredient, and the application of the microecological preparation in treating related diseases, which belongs to the field of biological medicine.

Description

technical field [0001] The invention relates to a preparation of Lactobacillus paracasei and its application, in particular to a microecological preparation prepared from Lactobacillus paracasei as the main active ingredient, and the application of the microecological preparation in treating related diseases, which belongs to the field of biological medicine. Background technique [0002] Lactobacillus paracasei plays an increasingly important role in the prevention and treatment of diseases, but the products containing Lactobacillus paracasei that are currently on the market have unsatisfactory effects. Therefore, screening for efficient Lactobacillus paracasei strains is an urgent problem to be solved at present , especially Lactobacillus paracasei strains with significant anti-allergic effect were screened. [0003] In recent years, the incidence of allergic diseases has been on the rise. According to the data of the World Health Organization, 22% of the world's people su...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/747A61P37/08A61P17/00A61P11/02A61P11/06A23L33/135A23L33/00A23K10/18
CPCA61K35/747A23V2002/00A23V2400/165A23V2200/318A23V2200/314A23V2200/304
Inventor 崔云龙郁聪
Owner QINGDAO EASTSEA PHARMA
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