A low-temperature biocontrol strain and its application

A bio-control strain and low-temperature technology, applied in the field of microorganisms, can solve the problems of slow growth, hinder the widespread application of microbial pesticides, reduce the application effect of biological pesticides, etc., and achieve the effect of high bacteriostatic rate and broad bacteriostatic spectrum.

Active Publication Date: 2018-12-25
INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the usual sowing season is early spring or autumn when the soil temperature is low, the microbial pesticide strains used today are all room temperature bacteria, which grow slowly or stop growing under low temperature conditions, which reduces the application effect of biological pesticides and hinders the widespread use of microbial pesticides. Therefore, the acquisition of low-temperature biocontrol strains can provide high-quality bacterial source materials for the research and development of low-temperature suitable biological fertilizers and biopesticides, and has potential research and development advantages in biological control

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  • A low-temperature biocontrol strain and its application
  • A low-temperature biocontrol strain and its application
  • A low-temperature biocontrol strain and its application

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Embodiment Construction

[0030] The technical solution of the present invention will be further described below in conjunction with the accompanying drawings, but it is not limited thereto. Any modification or equivalent replacement of the technical solution of the present invention without departing from the spirit and scope of the technical solution of the present invention should be covered by the present invention. within the scope of protection.

[0031] The invention provides a low-temperature biocontrol bacterial strain and application thereof, the specific contents are as follows:

[0032] 1) Screening and identification of low-temperature biocontrol strains:

[0033] The low-temperature biocontrol strain WSWGH-10 was isolated and screened from the winter permafrost in Hegang, Heilongjiang Province. The screening was carried out as follows: take 5g of permafrost and thaw it, grind it, put it into a conical flask containing 50ml of sterile water, shake it at 10°C for 3h, and dilute it with ste...

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Abstract

The invention discloses a low-temperature biocontrol bacterial strain and an application thereof. The low-temperature biocontrol bacterial strain is Bacillus methylotrophicus WSWGH-10, is deposited with CGMCC and is assigned the accession number CGMCC No.11541 at Oct, 21st, 2015 and at the deposition address Chaoyang District of Beijing, Beichen road, Yard 1, No.3. The biocontrol bacterial strain can grow and reproduce at low temperature, can product various antibacterial substances, such as siderophore, chitinase, lipopeptides, proteins and the like, and also has the capability of degrading cellulose. The bacterial strain has a wide antibacterial spectrum, can inhibit phytophthora sojae, sclerotinia sclerotiorum, pathogen of maize stalk rot, fusarium moniforme, ustilaginoidea virens, rice wilt pathogens, potato rhizoctonia bacteria, root rot pathogen of acanthopanax, pathogen of cucumber fusarium wilt, botrytis cinerea and such pathogenic fungus and tobacco bacterial disease pseudomonas angulate holland and such pathogenic bacteria. The bacterial strain also has the function of promoting plant growth.

Description

technical field [0001] The invention belongs to the technical field of microbes, and relates to a low-temperature biocontrol strain and its application. Background technique [0002] Crop disease is a natural disaster in agricultural production, which directly affects the yield and quality of crops, and causes huge losses. Due to the increasingly serious problems of chemical pesticide resistance and pollution, environmentally friendly microbial pesticides have become an important means of crop disease control. At present, microbial pesticides are mainly live bacterial preparations, and the unstable control effect in application is a common problem. Whether biocontrol bacteria can survive and reproduce in the soil environment is the key to their actual disease control effect. Since the usual sowing season is early spring or autumn when the soil temperature is low, the microbial pesticide strains used today are all room temperature bacteria, which grow slowly or stop growing...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01P1/00A01P3/00C12R1/07
Inventor 张淑梅刘宇帅姜威陈静宇孟利强胡基华李晶曹旭
Owner INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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