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Detection of Streptococcus pneumoniae

A Streptococcus pneumoniae detection and labeling technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problem that there is no provision for Streptococcus specific detection

Active Publication Date: 2021-05-14
GENOMICA SAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no specific probes are provided for the specific detection of different species of Streptococcus

Method used

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  • Detection of Streptococcus pneumoniae
  • Detection of Streptococcus pneumoniae
  • Detection of Streptococcus pneumoniae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0094] Samples of blood culture positive for S. pneumoniae were obtained and DNA was extracted therefrom by means of the NucliSENSeasy MAG kit from BioMerieux. The initial volume of blood culture used was 1 ml and the final volume after elution was 80 μl.

[0095] Next, take an elution volume of 5 μl and amplify it with a mixture of the following reagents:

[0096]

[0097]

[0098] Amplification products were denatured by incubation at 95°C for 10 minutes. Afterwards, in the presence of a standard hybridization solution, 5 μl of the denatured PCR product was mixed with the DNA containing the SEQ ID and SEQ ID Microarray incubation of probes.

[0099] After a series of standard washing and blocking steps, images were taken to obtain figure 1 A visualization of the final result is shown in .

Embodiment 2

[0101] Samples of blood cultures positive for S. light were obtained and the same procedure described in Example 1 was performed for S. pneumoniae. exist figure 2 A visualization of the final result is shown in .

Embodiment 3

[0103] Samples of blood cultures positive for oral Streptococcus were obtained and the same procedure described in Example 1 was performed for Streptococcus pneumoniae.

[0104] exist image 3 A visualization of the final result is shown in .

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Abstract

The invention is based on a method for the detection of Streptococcus pneumoniae in a sample, which allows a specific distinction between Streptococcus pneumoniae and other species of the Streptococcus genus. Furthermore, the present invention relates to (i) a kit comprising a microarray in which the probes of the present invention are disposed; (ii) the probe set itself; (iii) a microarray comprising the probe set; and (iv) the above Methods, kits, probe sets, and uses of microarrays for the detection of Streptococcus pneumoniae in a sample.

Description

[0001] Background of the invention [0002] Streptococcus pneumoniae is the causative agent of sepsis and it would be convenient to be able to specifically detect its presence in blood. Streptococcus pneumoniae is also a major cause of community-acquired pneumonia. It is very important to be able to distinguish S. pneumoniae from other members of the Streptococcus group, thereby determining whether any Streptococci present in the sample actually correspond to the pathogenic S. Other harmless streptococcal species that cause disease. [0003] Many attempts have been made to detect S. pneumoniae and differentiate it from other members of the Streptococcus group (Wessels et al., J. Clin. Microbiol. Apr. 2012, Vol. 50, No. 4, 1171-1177). These include culture tests and routine biochemical and phenotypic tests such as Gram-stained morphology, optoxin sensitivity, colony morphology, and alpha-hemolysis on sheep blood agar. Additional techniques used include: MALDI-TOF (matrix-assis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6837C12Q1/689C12N15/11
CPCC12Q1/6837C12Q1/689
Inventor 安娜·伊莎贝尔·莫拉加金塔尼利亚玛丽亚·路易莎·比利亚埃尔莫萨贾因
Owner GENOMICA SAU
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