Preparing method of feed antibiotic substitute rich in lipopeptid and Chinese herbal medicine effective ingredients

An effective ingredient, a technology of Chinese herbal medicine, applied in the field of preparation of feed antibiotic substitutes, can solve problems such as being unfavorable for Bacillus subtilis to produce lipopeptides, reducing the antibacterial activity of feed additives, and being unfavorable for degrading Chinese herbal medicine cell walls, etc. The effect of enhanced antibacterial activity and reduced production cost

Inactive Publication Date: 2016-06-29
GUANGDONG OCEAN UNIVERSITY
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Problems solved by technology

In this invention application, in order to make the prepared feed additive rich in small peptides, too much leftovers from aquatic products were added to the fermentation medium, and the carbon-nitrogen ratio in the fermentation medium was seriously unbalanced, which was not conducive to the production of Bacillus subtilis. Lipopeptides are also not conducive to the production of non-starch polysaccharides by Bacillus subtilis to degrade the cell walls of Chinese herbal medicines, thereby greatly reducing the antibacterial activity of the prepared feed additives

Method used

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Examples

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Effect test

Embodiment 1

[0026] Embodiment 1: the Bacillus subtilis ( Bacillus subtilis ) HL-1 was inserted into the LB liquid medium cooled after sterilization, and cultured at 37°C and 150r / min for 16h to prepare Bacillus subtilis ( Bacillus subtilis) HL-1 seed solution for later use; crush Astragalus membranaceus and pass through a 60-mesh sieve for later use. Mix 10 kilograms of pulverized astragalus, 500 grams of glucose, 700 grams of peptone, 600 grams of potassium chloride, 600 grams of magnesium chloride, 900 grams of dipotassium hydrogen phosphate and 100 kilograms of water to obtain a mixed fermentation medium; Medium culture is based on sterilizing at 121°C for 20 minutes and then ready for use; add 3 kg of Bacillus subtilis ( Bacillus subtilis ) HL-1 seed liquid; the fermentation temperature is 32°C, the sterile air intake is 2.0vvm, the rotation speed of the fermenter is 400rpm, and the fermentation time is 35h; after the fermentation, the fermentation liquid is filtered to remove the pr...

Embodiment 2

[0027] Embodiment 2: the Bacillus subtilis ( Bacillus subtilis ) HL-1 was inserted into the LB liquid medium cooled after sterilization, and cultured at 37°C and 150r / min for 16h to prepare Bacillus subtilis ( Bacillus subtilis ) HL-1 seed solution for later use; crush Astragalus membranaceus and Houttuynia cordata and pass through a 60-mesh sieve for later use. Mix 10 kg of compound Chinese herbal medicine (4 kg of Astragalus + 6 kg of Houttuynia cordata), 450 g of glucose, 650 g of peptone, 600 g of potassium chloride, 600 g of magnesium chloride, 900 g of dipotassium hydrogen phosphate and 100 kg of water to obtain Mixed fermentation medium; cultivated in the fermenter based on 121°C sterilization for 20 minutes for later use; add 3 kg of Bacillus subtilis ( Bacillus subtilis ) HL-1 seed liquid; the fermentation temperature is 30°C, the sterile air intake is 2.0vvm, the rotation speed of the fermenter is 400rpm, and the fermentation time is 42h; after the fermentation, the...

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Abstract

The invention discloses a preparing method of a feed antibiotic substitute rich in lipopeptid and Chinese herbal medicine effective ingredients. The preparing method comprises the main following steps that Chinese herbal medicine is smashed and screened in a 60-mesh mode for use; the smashed Chinese herbal medicine is added into a fermentation tank, and nutrient and water are added to serve as a fermentation culture medium; after being sterilized and cooled, the fermentation culture medium in the fermentation tank is inoculated with bacillus subtilis HL1 capable of generating anti-bacterial lipopeptid to be subjected to liquid aerobic fermentation; after fermentation is finished, fermentation liquor is subjected to filtration and precipitate removal to obtain supernatant liquor, the supernatant liquor is concentrated in a vacuum mode to obtain a concentrated solution, the concentration solution is adsorbed by an adsorption agent, and an adsorbing system can be prepared into the feed antibiotic substitute after being dried and smashed. The feed antibiotic substitute prepared through the method is rich in lipopeptid and Chinese herbal the medicine effective ingredients, has good properties of promoting growth and resisting disease, can replace feed antibiotic completely in the feed industry, and has a good application prospect.

Description

technical field [0001] The invention relates to a preparation method of a feed antibiotic substitute rich in lipopeptide and Chinese herbal medicine active ingredients, belonging to the technical field of feed additive preparation. Background technique [0002] At present, 97,000 tons of the approximately 210,000 tons of antibiotic raw materials produced in China each year are used in animal husbandry, accounting for 46.1% of the total annual output. Excessive use of antibiotics not only leads to the resistance of farmed animals to antibiotics and reduces the disease resistance of farmed animals, but also these antibiotics will enter the human body along the food chain, causing the human body to inadvertently produce drug-resistant bacteria, which will increase once they get sick. Difficulty and cost of treatment, and even "super bacteria" may be produced, and there is no cure. With the continuous improvement of people's quality of life, there are higher requirements for th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/30A23K10/12A23K20/195
CPCY02P60/87
Inventor 刘唤明洪鹏志刘颖
Owner GUANGDONG OCEAN UNIVERSITY
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