Method for measuring content of effective ingredients in cinobufagin extraction process

A technology for the extraction process and active ingredients, which is applied in the field of determination of the content of active ingredients in the extraction process of cinobufaginin, can solve the problems of affecting the uniformity and stability of the product, the inability to monitor in real time, and the difficulty of rapid determination, etc., so as to achieve rapid judgment and save Manpower and material resources, the effect of short measurement time

Inactive Publication Date: 2016-06-29
ANHUI CHINA RESOURCES JINCHAN PHARMA CO LTD
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Due to the many and complex components in the toad skin extract, it is difficult to quickly determine the content of its index quality control components
If there is a lack of effective quality detection means, it is impossible to monitor the changes in the content of active ingredients during the extraction process in real time, which will make it difficult to accurately control the production process and affect the uniformity and stability of the product

Method used

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  • Method for measuring content of effective ingredients in cinobufagin extraction process

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Collection of cinobufacin extract samples

[0025] Dried toad skin 500g, washed, extracted twice: for the first extraction, add 2500ml of pure water, heat to boil and then keep warm for 45min; After heating to boiling, keep warm and decoct for 30 minutes. For the first decoction, 5ml of the extract was collected every 2.5min, and 5ml of pure water was added. For the second decoction, 5ml of the extract was collected every 5min, and 5ml of purified water was added. The extraction experiment of different batches of cinobufacin was repeated 10 times, and 240 samples were obtained. One batch of data is randomly selected as the verification set, and the remaining 9 batches of samples are used as the calibration set to participate in the modeling.

[0026] 2. Determination of key indicators of cinobufacin extract samples

[0027] 1) Determination of solid content

[0028] To the weighing bottle (W 0 ), accurately add 2ml extract sample, weigh (W 1 ), evaporated to d...

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Abstract

The invention discloses a method for measuring the content of effective ingredients in the cinobufagin extraction process. The method comprises steps as follows: step 1) no fewer than 100 parts of cinobufagin extracting solution samples obtained in the extraction processes of different batches are collected; step 2) key indexes of the cinobufagin extracting solution samples are measured with a conventional analysis method respectively; step 3) a quantitative model between near infrared spectroscopy and each key index is established; step 4) the quantitative model is verified; step 5) key indexes of effective ingredients in an unknown sample are rapidly measured. The provided method for measuring the content of the effective ingredients in the cinobufagin extraction process can effectively solve the problem about rapid detection of the content of the effective ingredients in the cinobufagin extraction process, the cinobufagin extraction end point is rapidly judged, accurate control of process units is improved, finally, the quality stability of cinobufagin extracting solutions in different batches is finally improved, and the final product quality is guaranteed.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine pharmacy, and more specifically, the invention relates to a method for measuring the content of active ingredients in the extraction process of cinobufacin. Background technique [0002] Cinobufacin is derived from the skin of Bufobufogargarizans Cantor or Bufomelanostictus Schneider after extraction and separation. It is a second-class new drug developed by my country and a national traditional Chinese medicine protection variety. It has the pharmacological effects of regulating immunity, inhibiting tumor cell proliferation, and resisting hepatitis B virus. , has been widely used in the clinical treatment of anti-tumor, chronic hepatitis B and other diseases. [0003] At present, the production process of cinobufacin includes water extraction from toad skin, concentration, alcohol precipitation, secondary concentration, secondary alcohol precipitation, concentration and ointment collect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/15
CPCG01N33/15
Inventor 高波罗川
Owner ANHUI CHINA RESOURCES JINCHAN PHARMA CO LTD
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