A Method for Monitoring Insect Migration Regularity Using Double Immunolabeling
A technology of immune labeling and insects, applied in measuring devices, biological tests, material inspection products, etc., can solve the problem that the migration law of migratory populations is not clear and the law of migration of migratory populations is not clear
Inactive Publication Date: 2017-07-07
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology
However, the law of emigration of migratory populations and the law of re-migration of immigrant populations have not yet been clarified.
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[0053] Further illustrate the beneficial effect of the present invention by experimental case below:
[0054] Experimental case: the law of emigration of local populations and the law of re-migration of immigrated populations
[0055] The experiment was conducted for two years in 2010 and 2011 at the Ningjin County Experimental Base in Dezhou City, Shandong Province. Specific steps are as follows:
[0056] (1) Selection of experimental insects: Set-dwelling ladybugs, larvae and adults all use aphids as the main food source, and adults are migrating over summer;
[0057] Selection of experimental plants: alfalfa is a perennial flowering plant, which is an important silage, and also an important shelter for beneficial insects, especially natural predators, such as swarming ladybugs;
[0058] Selection and setting of experimental field: The experimental site is located in Ningjin County, Dezhou City, Shandong Province. The planting area of the experimental field is 2160m 2 ...
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The invention discloses a method for monitoring the insect migration rule through dual immunolabelling. The method includes the following steps of firstly, selecting migratory flight type insects; secondly, selecting protein markers; thirdly, conducting the occurrence situation survey on local populations of the target insects; fourthly, spraying the first protein marker and capturing the target insects; fifthly, spraying the second protein marker and capturing the target insects; sixthly, researching the emigration behavior rule of the local populations of the target insects and the re-migration flight behavior rule of immigrated populations. The method for researching the emigration rule of migration flight insects and the re-migration flight behavior rule of immigrated populations through the enzyme-linked immunolabelling technology is an effective method. The emigration rule of local migratory flight insects and the re-migration flight rule of immigrated insects can be clear, and therefore forecast, prevention and control of migratory flight insects can be better guided.
Description
technical field [0001] The invention belongs to the technical field of plant protection, and specifically relates to a method for monitoring insect migration rules using double immunolabeling, that is, using an enzyme-linked immunosorbent assay method to detect migratory insects with different marker proteins, so as to clarify the local migratory insects. The migration rules of flying insects and the re-migration rules of immigrated populations can guide the forecasting and control of agricultural migratory pests. Background technique [0002] Insect migration refers to the adaptive phenomenon of flying from one place of occurrence to another regularly in groups or scattered at a certain stage of growth and development in a certain season. Migratory insects often migrate long distances across provinces or even across borders, and often cause sudden disasters in agricultural, forestry and pastoral areas, posing a serious threat to economic production. For example, the desert...
Claims
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IPC IPC(8): G01N33/68G01N33/532
CPCG01N33/532G01N33/68
Inventor 万贵钧江守林刘勇波陈法军党志浩张逸飞代阳
Owner NANJING AGRICULTURAL UNIVERSITY