Method for determining residual quantity of 15 kinds of triazole type pesticides in fruit

A technology for pesticide residues and determination methods, which is applied in the field of determination of 15 kinds of triazole pesticide residues in fruits, achieves the effects of high enrichment efficiency, reduced matrix interference, and shortened analysis time

Inactive Publication Date: 2016-07-13
FRUIT TREE INST OF CHINESE ACAD OF AGRI SCI
View PDF1 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been no reports of 15 triazole pesticide residues in fruits using QuEChERS sample pretreatment method combined with GPC-GC/MS detection method. Therefore, the establishm

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for determining residual quantity of 15 kinds of triazole type pesticides in fruit
  • Method for determining residual quantity of 15 kinds of triazole type pesticides in fruit
  • Method for determining residual quantity of 15 kinds of triazole type pesticides in fruit

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0023] Embodiment 1: Determination of 15 kinds of triazole pesticide residues in fruit

[0024] 1. Sample pretreatment method

[0025] Accurately weigh 10g (accurate to 0.01g) of the homogenized fruit sample into a 50mL plastic centrifuge tube, add 10.0mL acetonitrile solution, 1.0g NaCl, 4.0g anhydrous MgSO 4 , shake for 1 min, centrifuge at 8000 r / min for 5 min, draw 2.0 mL of the supernatant and transfer it to a solution containing 50 mg PSA, 50 mg C 18 , 300 mg of anhydrous magnesium sulfate in a 10 mL centrifuge tube, vortex and mix for 1 min, and let stand. After the supernatant is passed through a 0.22 μm filter membrane, 1.0 mL of nitrogen is taken to dry it, and 1 mL of acetone / cyclohexane (3 / 7) is added. Reconstitute and mix.

[0026] 2. Preparation of matrix standard solution: 15 kinds of triazole pesticide standard products were prepared into standard solutions of flusilazole and propiconazole with different concentrations respectively with acetone, and niconazol...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Column lengthaaaaaaaaaa
The inside diameter ofaaaaaaaaaa
Login to view more

Abstract

The invention relates to a method for determining the residual quantity of 15 kinds of triazole type pesticides in fruit. The method comprises the following steps of (1) sample pretreatment: homogenated fruit samples are subjected to vibration shaking and high-speed centrifugation by using an acetonitrile solution, NaCl and anhydrous MgSO4 to obtain an extracting solution; then, PSA, C18 and anhydrous magnesium sulfate are selected as adsorbents to perform dispersive solid-phase extraction on the extracting solution; still standing, microporous filtering membrane passing and nitrogen gas drying through blowing are performed; next, redissolving is performed by an acetone and cyclohexane mixed solvent; uniform mixing and determination preparation are performed; (2) substrate standard solution preparation; (3) on-line gel color spectrum series gas chromatography-mass spectrum determination. The method has the characteristics of low background interference, high enrichment efficiency, high sensitivity, simplicity, convenience, high speed and accuracy, and can meet the residue monitoring requirements of the triazole type pesticides in the fruit.

Description

technical field [0001] The invention relates to a method for determining pesticide residues in fruits, in particular to a method for determining 15 kinds of triazole pesticide residues in fruits. Background technique [0002] Triazole pesticides belong to organic heterocyclic compounds. There are many kinds, including fungicides, insecticides, and plant growth regulators. They have excellent characteristics such as high efficiency, broad spectrum, long-acting, systemic absorption, and low toxicity. A class of high-efficiency fungicides with a wide range, flexible use methods, good control effects, and the most potential for development and application. However, with the increasing use of triazole pesticides in fruits, the food safety problems caused by their residues have attracted more and more attention. In order to ensure the safety of consumers, countries have established strict maximum residue limits of 0.010mg / kg for triazole pesticide residues in different fruits and...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 李海飞聂继云徐国锋毋永龙闫震李静李志霞匡立学李明强程杨沈友明关棣凯
Owner FRUIT TREE INST OF CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap