Method for measuring phenolic compounds in immature bitter oranges or fructus aurantii through SPE-HPLC
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A technology of phenolic compounds and citrus aurantii, which is applied in the field of drug detection, can solve the problems of not establishing a method for determining the content of components, and achieve the effects of suitable analysis conditions, good separation, and high enrichment rate
Inactive Publication Date: 2016-07-27
BEIJING UNIV OF CHINESE MEDICINE
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Shi Xuejiao et al. once proposed the star point design-response surface method to optimize the extraction process of the total phenolic acids of Aurantium citrus, and carried out content determination, but this method is mainly for the determination of the total phenolic content, and no method for the content determination of specific components has been established.
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Embodiment 1
[0018] 1 Instruments and reagents
[0019] Agilent HP1100 series liquid chromatograph (with autosampler), DAD ultraviolet detector, ChemStation chromatographic workstation, Supel-SelectHLB column (Waters, USA), Strata-X column (Phenomenex, USA), Strata-SDB-L small Column (Phenomenex, USA), Strata-C18E small column (Phenomenex, USA), TCQ-250 ultrasonic cleaner (Beijing Second Medical Equipment Factory), CP225D electronic balance (Sartorius, Germany).
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Abstract
The invention relates to a method for measuring phenolic compounds in immature bitter oranges or fructus aurantii through SPE-HPLC.The method specifically comprises the steps that powder of the immature bitter oranges or powder of fructus aurantii is taken, accurately weighed and placed into a conical flask with a cover, methyl alcohol is precisely added, ultrasonic extraction, cooling, well shaking and filtering are conducted, filtrate is precisely measured, pressure is reduced, the filtrate is recovered and dried, the product is dissolved with water, the mixture flows through a solid-phase extraction column which is balanced through methyl alcohol and water in advance, then, leaching with methyl alcohol, eluting with pure methyl alcohol, volume metering for the part eluted with pure methyl alcohol and filtering with a millipore filter membrane are sequentially conducted, and testing liquid is obtained; the phenolic compounds are measured.Compared with the prior art, the method has the advantage that as SPE is adopted for pretreating the sample, the effect of impurities on measuring is reduced; solid-phase extraction has the advantages that operation is easy and convenient, the enrichment ratio is high, less solvent is consumed, cost is low, and extraction and separation are integrated.In addition, chromatographic conditions established through the method have good resolution for the phenolic compounds to be measured, and analysis conditions are appropriate.
Description
technical field [0001] The invention belongs to the technical field of drug detection, and in particular relates to a method for measuring phenolic compounds in Fructus Aurantii or Fructus Aurantii by SPE-HPLC. Background technique [0002] Citrus aurantium, the name of traditional Chinese medicine; it belongs to the dried young fruit of Rutaceae plant sour orange and its cultivars or sweet orange. Lime, small tree of Rutaceae, citrus, with dense branches and leaves, many thorns, the thorns of the elongated branches are up to 8 cm. The leaf color is dark green, and the texture is quite thick. It is mainly produced in Sichuan, Jiangxi, Fujian, Jiangsu and other places. Pick or collect the self-falling fruit between May and June, cut it into two halves from the middle, sun-dry or low-temperature drying, and the smaller ones are directly sun-dried or low-temperature drying. When used, wash, suffocate, cut into thin slices, and dry. Used raw or fried with bran. "Medicines an...
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