Preparation method and application of active ingredient of Artemisia annua

Abstract

The invention relates to a preparation method and application of active ingredients of artemisia rupestris. The method comprises the steps that dry artemisia rupestris is smashed, extracted through an aqueous ethanol solution and separated and purified through a polyamide resin column, an eluted portion is extracted through an organic solvent, samples obtained on a trichloromethane layer are collected and then separated and purified for the second time through a silica gel column, recrystallization is conducted, and then three monomeric compounds are obtained, wherein the yield and content of casticin are 0.30 mg / g and 96%-98% respectively, the yield and content of 6-demethoxyl-4'-O-methyl capillarisin are 0.35 mg / g and 97%-99% respectively, and the yield and content of artemisetin are 0.06 mg / g and 95%-97%. In-vitro anti-influenza virus experiments show that the artemisia rupestris active ingredients obtained through the method have the certain activity of resisting an A influenza virus, a stephania tetrandra influenza virus, a 359 influenza virus and a 95 (H3N2) influenza virus and are potential novel anti-influenza virus lead compounds. According to the method, casticin, 6-demethoxyl-4'-O-methyl capillarisin and artemisetin are simultaneously acquired from the artemisia rupestris, and the advantages of being low in cost, easy and convenient to operate, efficient and stable are achieved.

Description

Technical field [0001] The invention relates to the preparation and use of the active ingredient of Artemisia sphaerocephala against influenza virus. Background technique: [0002] Artemisia rupestris L. is a plant belonging to the genus Artemisia L. of the Compositae family. It is an indigenous medicinal material in Xinjiang and its Uyghur medicine name is Zihuo Aimini. Mainly distributed in Xinjiang, but also distributed in Central Asia, Europe and other places. Xinjiang is mainly distributed along the Tianshan Mountains and Altai Mountains, and generally grows in subalpine grasslands and open coniferous forests at an altitude of 1600-3000m. Artemisia xinjiangensis is cold in nature and has a bitter taste. It has the functions of clearing away heat and reducing fever, reducing inflammation and pain, cooling blood and detoxification. Artemisia xinjiangensis has a high medicinal value. The whole herb is mostly used as medicine in the folk. It has anti-allergic, anti-oxidant, an...

AI Technical Summary

Problems solved by technology

The research on the anti-influenza virus effect of Artemisia argyi is relatively in-depth, but due to the complex structure of the monomer components, the difficulty of synthesis, and the lack of access to it, the research on its pharmacological activity is mostly concentrated on the effective parts, and the pharmacological activity of its monomer components The research on its mechanism of action is not yet in-depth, and there is currently no research on the pharmacological activity of other monomeric components in Artemisia japonica except artemisinic acid

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