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Detection primer composition, reagent kit and method for mitochondria drug-induced deafness

A technology for drug-induced deafness and detection kits, which is applied to the determination/inspection of microorganisms, biochemical equipment and methods, recombinant DNA technology, etc. Achieve the effect of high yield, low cost, and rapid amplification reaction

Inactive Publication Date: 2016-08-17
BEIJING JINQI BIOLOGICAL TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The technical problem to be solved by the present invention is that the existing detection of mitochondrial gene site mutations has problems such as high cost, long detection cycle, cumbersome operation, etc., which is not conducive to the wide-scale popularization of clinical testing

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  • Detection primer composition, reagent kit and method for mitochondria drug-induced deafness
  • Detection primer composition, reagent kit and method for mitochondria drug-induced deafness
  • Detection primer composition, reagent kit and method for mitochondria drug-induced deafness

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Embodiment Construction

[0053] In order to better understand the present invention, the present invention will be further described below in conjunction with specific embodiments and accompanying drawings, but the protection scope of the present invention is not limited.

[0054] A detection primer composition for mitochondrial drug-induced deafness, comprising:

[0055] Forward outer primer F3:

[0056] 5'-TGGGCTACATTTTCTACCC-3' (SEQ ID No. 1);

[0057] Reverse Outer Primer B3:

[0058] 5'-ACACTTACCATGTTACGACT-3' (SEQ ID No.2);

[0059] Forward inner primer FIP:

[0060] 5'-AGCACTCTACTCTTAGTTTACTGCTCAGAAAACTACGATAGCCCTT-3' (SEQ ID No. 3);

[0061] Reverse inner primer BIP:

[0062] 5'-CCGTCACCCTCCTCAAGTATTGTCTCCTCTATATAAATGCGTA-3' (SEQ ID No. 4); and

[0063] Peptide nucleic acid sequence PNA-4C used to block the C allele at position 1494:

[0064] 5'-CCGTCACCCTCCTCA-3' (SEQ ID No. 5);

[0065] Peptide nucleic acid sequence PNA-4T used to block the T allele at position 1494:

[0066] 5'-CCC...

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Abstract

The invention relates to the technical field of biological detection, in particular to detection primer composition, reagent kit and method for mitochondria drug-induced deafness. With the PNA Clamp LAMP technology having PNA and LAMP combined, C-alleles of 1494 loci are closed by PNA-4C, T-alleles of 1494 loci are closed by PNA-4T, A-alleles of 1555 loci are closed by PNA-5A, and G-alleles of 1555 loci are closed by PNA-5G. In this way, corresponding LAMP reaction cannot go on for the purpose of detecting sudden change of gene loci of the mitochondria. The method is simple in steps, good in specificity, convenient to identify, low in cost and convenient in large-scale popularization of clinical detection.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a detection primer composition, kit and method for mitochondrial drug-induced deafness. Background technique [0002] Drug-induced deafness (referred to as drug-induced deafness) refers to the deafness caused by the use or contact of certain ototoxic drugs. These drugs (including aminoglycoside antibiotics, non-aminoglycoside antibiotics, salicylates, etc.) It has toxic and side effects on the human auditory nerve, can damage the cochlea, and generally causes bilateral and permanent deafness, which is mostly irreversible. [0003] According to the results of China’s second national sample survey of disabled people in 2006, there are about 20.04 million people with hearing disabilities, accounting for 24.16% of the total number of 82.96 million people with disabilities in the country, and as many as 8 million people are deaf due to drugs. Among them, the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6858C12Q1/6883C12Q2600/156C12Q2531/119C12Q2525/107
Inventor 汪大为张井存
Owner BEIJING JINQI BIOLOGICAL TECH CO LTD
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