A reagent cartridge provided with a push-type magnetic bead transfer mechanism

Abstract

A reagent cartridge provided with a push-type magnetic bead transfer mechanism comprises a first cavity and a second cavity arranged adjacently and an interval area separating the first cavity and the second cavity; the interval area A accommodating cavity is formed in the accommodating cavity, and a pressing magnetic bead transfer mechanism for transferring the magnetic beads in the first cavity to the second cavity is arranged in the accommodating cavity, and the pressing magnetic bead transfer mechanism is sealed and fitted in the accommodating cavity. In the cavity, the push-type magnetic bead transfer mechanism includes a rotor and a push rod that pushes the rotor to rotate, and the bottom of the rotor is provided with a cylindrical rotating structure driven by the rotor. In the solution of the present invention, a pressing magnetic bead transfer mechanism is arranged between adjacent cavities, so that the magnetic beads and the materials to be extracted adsorbed on the magnetic beads are transferred sufficiently and efficiently; in addition, the reagent cartridge of the present invention not only The utility model has the advantages of simple structure and convenient operation, which can effectively reduce the complexity of the equipment used with it.

Description

Technical field [0001] The invention relates to consumables for the preparation and detection of nucleic acid or protein, and in particular to a reagent cartridge provided with a pressing type magnetic bead transfer mechanism. Background technique [0002] With the transformation of medical models and the continuous development of individualized medications, the medical laboratory industry urgently needs fast and accurate detection methods, and molecular detection exerts its unique advantages. The basis of molecular detection is to analyze the tissue cells, hair, anticoagulant blood or dried blood stains of the subject, as well as the genes and their expression products in the formaldehyde-fixed and paraffin-embedded tissues, and complete nucleic acid (DNA and RNA) at the molecular level. ) Detection, once the disease occurs or even before the symptoms, signs and biochemical changes appear, accurate detection can be made. [0003] At present, molecular detection technologies mainl...

AI Technical Summary

Problems solved by technology

At present, blood routine, cytology, pathology and immunology and other testing methods are all developing in the direction of automation, integration and standardization, but due to the technical complexity of molecular testing, the fully automated instrument platform "from sample to result" Few or many technical problems that are difficult to solve

For example, the spin column method or magnetic bead method used in the field of biotechnology for nucleic acid extraction generally requires four steps of lysis, binding, rinsing, and elution, plus subsequent nucleic acid molecule hybridization, polymerase chain reaction (PCR ) and biochips and other detection steps, it is very difficult to realize the fully automated instrument "from sample to result". It is obvious that the manual material transfer method used in the prior art is not only cumbersome to operate, but also time-consuming and labor-intensive.

What's more, the whole operation process is easy to cause pollution, which affects the purity of the extract, and it is difficult to transfer the material fully and efficiently, which affects the experimental results.

Therefore, in view of the technical complexity of molecular detection itself, there are few fully automated instrument platforms for "from sample to result" or there are many difficult technical problems. In order to avoid problems in the prior art such as high cost, poor sensitivity, complex structure, overly complicated operation and related detection equipment, etc.

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