Method for extracting oleanolic acid and hederagenin in clematis root
A technology of helexin and oleanolic acid, applied in the field of laboratory testing, can solve the problems of low determination concentration, complicated operation and the like
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[0020] Step 1: After crushing Clematis clematis, extract by ASE extraction method to obtain extract;
[0021] Wherein, the step 1 is specifically:
[0022] S11: Mix 1g of Clematis with 2g of quartz sand after crushing;
[0023] S12: Add 3 g of the mixture in S11 to the extraction tank covered with 1 g of diatomaceous earth, then add quartz sand to make the solid in the extraction tank reach the mouth of the extraction tank, cover the extraction tank for extraction; collect the extract;
[0024] The extraction is carried out in two steps, namely a degreasing step and an extraction step; the solution obtained in the degreasing step is discarded, and the solution obtained in the extraction step is used as the extract;
[0025] The control parameters of the degreasing step are as follows: the extraction solvent is ethyl acetate and methyl acetate; the extraction temperature is 110°C; the static extraction time is 3 minutes; the flushing volume is 100%; the number of static cycles...
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