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Method for extracting oleanolic acid and hederagenin in clematis root

A technology of helexin and oleanolic acid, applied in the field of laboratory testing, can solve the problems of low determination concentration, complicated operation and the like

Inactive Publication Date: 2016-09-21
WUZHOU INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problem that the operation in the pharmacopoeia method is too complicated, we have obtained a rapid extraction method through ASE to separate and test oleanolic acid in Clematis clematis through a large number of experiments. Specifically, the extraction process is divided into impurity removal And extraction step, solvent is ethyl acetate in the impurity removal step, and the solvent in the extraction step is dilute ethanol, and it can very well measure the oleanolic acid in Clematis clematis, and it is consistent with the precision that pharmacopoeia method measures, but in Find in practical application, when adopting this ASE rapid extraction method to measure helexin simultaneously, its measured concentration is on the low side, although the chemical formula of hederiden and oleanolic acid is C 30 h 48 o 3 , but in the extraction process, the extraction solvent needs to be further adjusted in order to simultaneously determine helexin and oleanolic acid

Method used

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  • Method for extracting oleanolic acid and hederagenin in clematis root
  • Method for extracting oleanolic acid and hederagenin in clematis root
  • Method for extracting oleanolic acid and hederagenin in clematis root

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Embodiment 1

[0020] Step 1: After crushing Clematis clematis, extract by ASE extraction method to obtain extract;

[0021] Wherein, the step 1 is specifically:

[0022] S11: Mix 1g of Clematis with 2g of quartz sand after crushing;

[0023] S12: Add 3 g of the mixture in S11 to the extraction tank covered with 1 g of diatomaceous earth, then add quartz sand to make the solid in the extraction tank reach the mouth of the extraction tank, cover the extraction tank for extraction; collect the extract;

[0024] The extraction is carried out in two steps, namely a degreasing step and an extraction step; the solution obtained in the degreasing step is discarded, and the solution obtained in the extraction step is used as the extract;

[0025] The control parameters of the degreasing step are as follows: the extraction solvent is ethyl acetate and methyl acetate; the extraction temperature is 110°C; the static extraction time is 3 minutes; the flushing volume is 100%; the number of static cycles...

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Abstract

The invention discloses a method for extracting oleanolic acid and hederagenin in clematis root. The method comprises the following steps: step 1, crushing the clematis root and then mixing the crushed clematis root with a proper amount of quartz sand; step 2, adding the mixture obtained in the previous step into an extraction tank where diatomite is laid, adding quartz sand until solids in the extraction tank reach the mouth of the extraction tank, covering a cover of the extraction tank, carrying out extraction and collecting extract, wherein extraction is carried out in two steps, i.e., a degreasing step and an extraction step; and step 3, subjecting the extract to centrifugation so as to obtain supernatant. The method for extracting oleanolic acid and hederagenin in the clematis root in the invention can simultaneously extract oleanolic acid and hederagenin in the clematis root, is thorough in extraction and reaches extraction effect described in pharmacopeia.

Description

technical field [0001] The invention relates to the field of laboratory detection, in particular to a method for extracting oleanolic acid and hedera saponin from Clematis clematis. Background technique [0002] In 2010, the Pharmacopoeia recorded the method of extracting hedera saponin from Clematis clematis: take about 4g of the powder of this product (passed through a No. 4 sieve), weigh it accurately, put it in a Soxhlet extractor, add an appropriate amount of ethyl acetate, and heat to reflux for 3 hours , discard the ethyl acetate solution, evaporate the dregs to dry the solvent, transfer it together with the filter paper cylinder to a conical flask, add 50ml of dilute ethanol precisely, weigh it, heat and reflux for 1 hour, let it cool, then weigh it, and use dilute ethanol Make up for the lost weight, shake well, filter, accurately measure 25ml of the filtrate, put it on a water bath and evaporate to dryness, add 30ml of 2mol / L hydrochloric acid solution to the resid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 陈学松欧妮陈翠玲钟水桥李仁乔
Owner WUZHOU INST FOR FOOD & DRUG CONTROL
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