Method for extracting oleanolic acid and hederagenin in clematis root

Abstract

The invention discloses a method for extracting oleanolic acid and hederagenin in clematis root. The method comprises the following steps: step 1, crushing the clematis root and then mixing the crushed clematis root with a proper amount of quartz sand; step 2, adding the mixture obtained in the previous step into an extraction tank where diatomite is laid, adding quartz sand until solids in the extraction tank reach the mouth of the extraction tank, covering a cover of the extraction tank, carrying out extraction and collecting extract, wherein extraction is carried out in two steps, i.e., a degreasing step and an extraction step; and step 3, subjecting the extract to centrifugation so as to obtain supernatant. The method for extracting oleanolic acid and hederagenin in the clematis root in the invention can simultaneously extract oleanolic acid and hederagenin in the clematis root, is thorough in extraction and reaches extraction effect described in pharmacopeia.

Description

technical field [0001] The invention relates to the field of laboratory detection, in particular to a method for extracting oleanolic acid and hedera saponin from Clematis clematis. Background technique [0002] In 2010, the Pharmacopoeia recorded the method of extracting hedera saponin from Clematis clematis: take about 4g of the powder of this product (passed through a No. 4 sieve), weigh it accurately, put it in a Soxhlet extractor, add an appropriate amount of ethyl acetate, and heat to reflux for 3 hours , discard the ethyl acetate solution, evaporate the dregs to dry the solvent, transfer it together with the filter paper cylinder to a conical flask, add 50ml of dilute ethanol precisely, weigh it, heat and reflux for 1 hour, let it cool, then weigh it, and use dilute ethanol Make up for the lost weight, shake well, filter, accurately measure 25ml of the filtrate, put it on a water bath and evaporate to dryness, add 30ml of 2mol / L hydrochloric acid solution to the resid...

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problem that the operation in the pharmacopoeia method is too complicated, we have obtained a rapid extraction method through ASE to separate and test oleanolic acid in Clematis clematis through a large number of experiments. Specifically, the extraction process is divided into impurity removal And extraction step, solvent is ethyl acetate in the impurity removal step, and the solvent in the extraction step is dilute ethanol, and it can very well measure the oleanolic acid in Clematis clematis, and it is consistent with the precision that pharmacopoeia method measures, but in Find in practical application, when adopting this ASE rapid extraction method to measure helexin simultaneously, its measured concentration is on the low side, although the chemical formula of hederiden and oleanolic acid is C 30 h 48 o 3 , but in the extraction process, the extraction solvent needs to be further adjusted in order to simultaneously determine helexin and oleanolic acid

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