Method for cultivating ludisia discolor by means of orchid mycorrhizae

Abstract

The invention provides a method for cultivating ludisia discolor by means of orchid mycorrhizae. Orchid mycorrhizae is cleaned with tap water and subjected to surface sterilization with ethyl alcohol of 75%, nodular tissue is cut off on an ultra-clean work table and placed in a fungus plate medium till hyphae grow, and hyphae are extracted and placed in the same plate medium for purification cultivation; cultivation is conducted at 25-30 DEG C for 7-8 days till the plat medium is covered with hyphae, and hypha blocks on a plate are cut and placed in a mixed cultivation fungus sack for inoculation; cultivation is conducted at 24-28 DEG C for 35-40 days till hyphae grow out of the fungus sack; the fungus sack is mixed with a matrix where orchid was planted to serve as a ludisia discolor cultivation medium; then the medium is inoculated with ludisia discolor tissue cultivation seedlings for cultivation. Wild ludisia discolor tissue cultivation seedlings are adopted, the matrix where orchid was planted is mixed with the endophytic fungus mixed medium cultivated artificially to cultivate ludisia discolor, plant height and weight are increased remarkably, product quality is improved remarkably, and then the medical value of ludisia discolor can be better developed and utilized.

Description

technical field [0001] The invention belongs to the field of plant cultivation, and in particular relates to a method for cultivating male stone pine by using orchid root fungus. Background technique [0002] Gongshisong: Ludisia discolor ( Ker-Gawl. ) A. Rich., a plant of Orchidaceae, is a herbal medicine with local characteristics in southern Fujian. It has the effects of clearing heat and cooling blood, promoting body fluid and reducing fire, diuresis and collaterals, and relieving cough. It can be used to treat persistent high fever, sore throat, swelling and pain without name, spleen and stomach diseases and inflammation. "Chinese Materia Medica" records its efficacy: "moisten the lungs, invigorate the spleen, calm the nerves, and treat mainly tuberculosis and hemoptysis, neurasthenia, and loss of appetite." Male stone pine has strict requirements on the growth environment, and often grows in damp rock crevices, and the growth place is mostly rich in endophytic fungi....

AI Technical Summary

Problems solved by technology

[0003] Artificial cultivation According to some distributors' investigations on the artificial cultivation of fresh plants of male stone pine, because the male stone pine has strict requirements on the growth environment, there is no more authoritative mature technology to refer to, and few people have carried out cultivation trial research on artificial cultivation. Planting Techniques. The male stone pine sold in the market is generally cultivated for short-term in three forms: sand culture, hydroponics and soil culture by purchasing fresh plants. Sand culture is used by most people because of its good air permeability, sufficient mineral nutrition, less water accumulation and no root rot, etc., but the general technical content is low, and the growth of biological yield is not obvious. Moreover, sand culture is not used for large-scale seedling cultivation, but is mainly used to properly extend the life cycle of fresh plants, and arrange the supply of fresh plants according to sales volume to ensure long-term uninterrupted supply. In production practice, there is no precedent for large-scale seedling cultivation using asexual reproduction techniques such as cuttings and tissue culture.

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