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Application of monkey earring extract in the preparation of anti-multidrug-resistant Pseudomonas aeruginosa drugs

A Pseudomonas aeruginosa, multi-drug resistance technology, applied in antibacterial drugs, plant/algae/fungus/moss components, pharmaceutical formulations, etc., can solve the antibacterial sensitization effect of Pseudomonas aeruginosa not mentioned problems, to achieve the effect of low cost and low side effects

Active Publication Date: 2019-09-17
HUACHENG PHARMA FACTORY GAUNGZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese patent CN103385912A discloses that the extract of monkey earrings has anti-MRSA effect and antibiotic sensitization effect, but the patent does not mention the antibacterial effect of the monkey earrings extract on multidrug-resistant Pseudomonas aeruginosa and the increase in combination with antibiotics. Sensitization

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  • Application of monkey earring extract in the preparation of anti-multidrug-resistant Pseudomonas aeruginosa drugs
  • Application of monkey earring extract in the preparation of anti-multidrug-resistant Pseudomonas aeruginosa drugs
  • Application of monkey earring extract in the preparation of anti-multidrug-resistant Pseudomonas aeruginosa drugs

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Preparation method of monkey earring extract

[0021] Monkey earrings (Pithecellobium clypearia Benth) were provided by Guangzhou Huacheng Pharmaceutical Factory. Take an appropriate amount of monkey earring medicinal material, grind it into coarse powder, reflux twice with water or ethanol for 2 hours each time, and filter; combine the filtrates and concentrate to obtain an extract (water or ethanol extract). Suspend the extract with water, extract with ethyl acetate three times, combine the ethyl acetate extracts, and concentrate to obtain the ethyl acetate extract.

[0022] The ethanol extract can be prepared by refluxing 10-95% ethanol.

Embodiment 2

[0024] Bacteriostatic and bactericidal tests of monkey earring water extract against multidrug-resistant Pseudomonas aeruginosa and the sensitizing effect of combined with levofloxacin, imipenem, amikacin, ceftazidime, and cefoperazone.

[0025] 1. Experimental method

[0026] 1) Determination of minimum inhibitory concentration (MIC):

[0027] Monkey earring water extract, levofloxacin (LVX), imipenem (IMP), amikacin (AMK), ceftazidime (CAZ), cefoperazone (CFP) were subjected to a series of times in MH broth medium Ratio dilution, 50 μl per well, adjust the inoculum to 1.0×10 6 CFU / ml, 50 μl bacterial solution per well. Incubate at 35°C; for 24 hours, the lowest concentration of antibacterial drug without precipitation is its minimum inhibitory concentration (MIC).

[0028] 2) Determination of minimum bactericidal concentration (MBC):

[0029] Using the plate coating counting method, draw 50ul of bacterial suspension from the aseptic growth hole in item 1) onto the blood ...

Embodiment 3

[0076] Bacteriostasis and bactericidal test of 10% ethanol extract from monkey earrings against multi-drug resistant Pseudomonas aeruginosa and investigation on the sensitizing effects of combination with levofloxacin, imipenem, amikacin, ceftazidime and cefoperazone respectively.

[0077] 1. Experimental method: measure and evaluate by the method under the embodiment 2 of the present invention.

[0078] 2. Experimental results:

[0079] Table 13 shows the in vitro antibacterial test results of 10% ethanol extract of monkey earrings and five antibiotics (levofloxacin, imipenem, amikacin, ceftazidime, and cefoperazone) against multidrug-resistant Pseudomonas aeruginosa.

[0080] See Table 14 for the in vitro bactericidal test results of 10% ethanol extract of monkey earrings against multi-drug resistant Pseudomonas aeruginosa.

[0081] After statistical analysis, the MIC50 and MIC90 of 10% ethanol extract of monkey earrings and five antibiotics against multi-drug resistant Pse...

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Abstract

The invention discloses application of a folium archidendri clypeariae extract in preparation of a multidrug-resistant pseudomonas aeruginosa drug and such antibiotic sensitizing drug. The folium archidendri clypeariae extract is prepared by extracting folium archidendri clypeariae coarse powder with water or an aqueous alcohol solution, and extracting the extracting solution with ethyl acetate to obtain the extract, i.e., a target product. The invention firstly discloses the application of the folium archidendri clypeariae extract in preparation of the multidrug-resistant pseudomonas aeruginosa drug and such antibiotic sensitizing drug. The folium archidendri clypeariae extract can be used as a natural antibacterial drug or an antibiotic sensitizing agent, and applied to treatment of diseases caused by pseudomonas aeruginosa. The folium archidendri clypeariae extract as a traditional Chinese medicine extract also has the advantages of low cost, low side effects and the like.

Description

technical field [0001] The invention relates to a new application of monkey earring extract in preparing anti-drug-resistant bacteria medicine. Background technique [0002] The 21st century is the era of multi-drug-resistant bacteria. After 60 years of clinical application of antibiotics, more and more nosocomial infections and multi-drug-resistant bacterial infections have become a major problem in clinical antibacterial treatment. In 1961, Jerons reported the first clinical case of A-resistant Patients with methicillin Staphylococcus aureus (MRSA) infection, currently MRSA infection has gradually spread all over the world; the distribution of main drug-resistant bacteria in China's bacterial resistance monitoring clinic in 2011, Escherichia coli, Klebsiella pneumoniae producing extended-spectrum β -Lactamase (ESBL) strains averaged 50.7% and 38.5% respectively, and the change and status quo of their drug resistance have attracted much attention. In addition, according to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/48A61P31/04A61K31/546A61K31/7036A61K31/407A61K31/5383
Inventor 王永刚刘翀周倩李沛波苏薇薇彭维
Owner HUACHENG PHARMA FACTORY GAUNGZHOU