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A kind of amorphous silk fibroin nanofiber solution and preparation method thereof

A nanofiber and amorphous silk technology, which is applied in the field of amorphous silk fibroin nanofiber solution and its preparation, can solve the problem that the preparation method of silk protein nanofiber aqueous solution has not been reported, it is difficult to obtain silk protein, and the silk protein aqueous solution cannot be obtained, etc. problem, achieve the effect of no organic solvent residue, good biocompatibility and simple process

Active Publication Date: 2018-03-27
苏州至明至信生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

By designing formic acid-calcium chloride and formic acid-lithium bromide mixed solvents, the dissolution can be realized while retaining the nanostructure of silk protein, and different types of silk protein materials have been prepared. However, in the above patents and technologies, with formic acid and calcium chloride , the precipitation of lithium bromide, the silk protein will quickly reassemble and form a beta-sheet structure, resulting in the solidification of its gel, and the aqueous solution of silk protein cannot be obtained. At the same time, silk protein has different structures in the above system, and it is difficult to obtain silk protein with uniform structure
So far, the aqueous solution of silk protein nanofibers with amorphous structure and its preparation method have not been reported

Method used

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  • A kind of amorphous silk fibroin nanofiber solution and preparation method thereof
  • A kind of amorphous silk fibroin nanofiber solution and preparation method thereof
  • A kind of amorphous silk fibroin nanofiber solution and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] S1. Add 2.5ml 98% formic acid to 40ml 8.4M potassium bromide solution, stir and mix;

[0049] S2, then add 0.6g degummed silk, and extrude the glass rod until fully soaked to obtain a mixed solution;

[0050] S3. Put the mixed solution in an oven, and place it at 60° C. for 4 hours to obtain a mixed solution;

[0051] S4. Then put the mixed solution into a dialysis bag, and dialyze in deionized water at 20° C. for 2 days, during which the water is changed every 2 hours.

[0052] S5. Perform centrifugation at a centrifugation rate of 9000 rpm for 20 minutes, and then take the supernatant, which is the amorphous silk fibroin nanofiber solution.

Embodiment 2

[0054] (1) Add 1ml 98% formic acid to 48ml 7.2M potassium bromide solution, stir and mix;

[0055] (2) Then add 1.8g of degummed silk, and extrude the glass rod until fully soaked to obtain a mixed solution;

[0056] (3) Put the mixed solution in an oven and place it at 75°C for 2 hours to obtain a mixed solution;

[0057] (4) The mixed solution was then put into a dialysis bag, and dialyzed in deionized water at 10°C for 4 days, during which the water was changed every 4 hours.

[0058] (5) Centrifuge at a speed of 6000 rpm for 40 minutes, and then take the supernatant, which is the amorphous silk fibroin nanofiber solution.

Embodiment 3

[0060] (1) Add 1.5ml 98% formic acid to 45ml 8M potassium bromide solution, stir and mix;

[0061] (2) Then add 1g of degummed silk, and extrude the glass rod until fully soaked to obtain a mixed solution;

[0062] (3) Put the mixed solution in an oven and place it at 45°C for 8 hours to obtain a mixed solution;

[0063] (4) Then put it into a dialysis bag, and dialyze in deionized water at 2°C for 2 days, during which time the water is changed every 2 hours.

[0064] (5) Centrifuge at a speed of 10,000 rpm for 10 minutes, and then take the supernatant, which is the amorphous silk fibroin nanofiber solution.

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Abstract

The invention discloses an amorphous silk fibroin nanofiber solution and a preparation method thereof. The amorphous silk fibroin nanofiber has a diameter below 20nm and a length between 200-500nm. In the preparation process, a solution which has a uniform structure and mainly contains amorphous silk fibroin nanofibers is finally formed by cooperative control of a solvent system, quality of fibroin protein fibers, dissolving temperature, dissolving time, temperature during dialyzing process, water changing time, centrifuging speed and centrifuging time. Furthermore, by adopting the preparation method with a simple process, the prepared product does not have organic solvent residue and has good biocompatibility.

Description

technical field [0001] The invention relates to the technical field of nanofiber solutions, in particular to an amorphous silk fibroin nanofiber solution and a preparation method thereof. Background technique [0002] As a natural protein, the functional applications of silk fibroin in the fields of tissue engineering, drug release, and microdevices have been widely explored. The formation of different forms of regenerated silk fibroin, including scaffolds, films, gels, powders, and adhesives, is a prerequisite for these applications. Recently, with the expansion and deepening of the application field, higher and higher requirements are put forward for the control of silk protein microstructure. However, as the basis for the preparation of silk protein-based materials, there is basically no uniform nanostructure of silk protein in silk protein aqueous solution. , making it very difficult to further realize the microstructure design of silk protein materials, which limits th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/03C08L89/00
CPCC08J3/03C08J2389/00
Inventor 吕强肖丽媖
Owner 苏州至明至信生物科技有限公司