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A kind of metastable silk fibroin nanoparticle, and the preparation method of its solution

A nanoparticle and silk fibroin technology, applied in the field of nanoparticles, can solve the problems of difficulty in obtaining silk protein with uniform structure and become gel, and achieve high size uniformity, no organic solvent residue, and good biocompatibility. Effect

Active Publication Date: 2019-03-26
苏州至明至信生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in such patents and technologies, the solution rapidly turns into a gel as the salt ions are removed during dialysis
At the same time, silk protein has different structures in the above system, and it is difficult to obtain silk protein with uniform structure.
But it shows that it is possible to prepare silk fibroin materials that maintain a special metastable structure in aqueous solution by adjusting the solvent system
In the prior art, there is no precedent for directly dissolving silk to prepare metastable silk fibroin nanoparticles through the design of solvent system

Method used

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  • A kind of metastable silk fibroin nanoparticle, and the preparation method of its solution
  • A kind of metastable silk fibroin nanoparticle, and the preparation method of its solution
  • A kind of metastable silk fibroin nanoparticle, and the preparation method of its solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] S1. Add 2.5ml 88% formic acid to 40ml 8.5M potassium bromide solution, stir and mix;

[0049] S2, then add 0.5g of degummed silk, and extrude the glass rod until fully soaked to obtain a mixed solution;

[0050] S3. Put the mixed solution in an oven, and place it at 60° C. for 4 hours to obtain a mixed solution;

[0051] S4. Then put the mixed solution into a dialysis bag, and dialyze in deionized water at 20° C. for 3 days, during which the water is changed every 2 hours.

[0052] S5. Perform centrifugation at a centrifugation rate of 9000 rpm and a centrifugation time of 20 min, then collect the precipitate, and redisperse the precipitate with ultrapure water to finally obtain a metastable silk fibroin nanoparticle solution.

Embodiment 2

[0054] (1) 1ml 98% formic acid was added to 48ml 7M potassium bromide solution, stirred and mixed;

[0055] (2) Then add 2g of degummed silk, and extrude the glass rod until fully soaked to obtain a mixed solution;

[0056] (3) Put the mixed solution in an oven and place it at 75°C for 2 hours to obtain a mixed solution;

[0057] (4) The mixed solution was then put into a dialysis bag, and dialyzed in deionized water at 10°C for 4 days, during which the water was changed every 4 hours.

[0058] (5) Centrifuge at a speed of 6000 rpm and a centrifugation time of 40 min, then collect the precipitate and redisperse the precipitate with ultrapure water to finally obtain a metastable silk fibroin nanoparticle solution.

Embodiment 3

[0060] (1) Add 1.5ml 98% formic acid to 45ml 8M potassium bromide solution, stir and mix;

[0061] (2) Then add 1g of degummed silk, and extrude the glass rod until fully soaked to obtain a mixed solution;

[0062] (3) Put the mixed solution in an oven and place it at 45°C for 8 hours to obtain a mixed solution;

[0063] (4) Then put it into a dialysis bag, and dialyze in deionized water at 2°C for 2 days, during which time the water is changed every 2 hours.

[0064] (5) Perform centrifugation at a centrifugal rate of 10000 rpm for 10 min, then collect the precipitate and redisperse the precipitate with ultrapure water to finally obtain a metastable silk fibroin nanoparticle solution.

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Abstract

The invention discloses a metastable state silk fibroin nano-particle, and a preparation method of a solution thereof. The diameter of the metastable state silk fibroin nano-particle is between 100-200nm, and the relatively high dimensional homogeneity, the good dispersibility and the difficult accumulation facilitate the drug-loaded silk fibroin nano-particle which is taken as a drug carrier to build different functions and release actions; in a process of preparing the metastable state silk fibroin nano-particle solution, through cooperative control on the solvent system, the silk fibroin fiber mass, the dissolving temperature, the dissolving time, the temperature in a dialysis process, the water changing time, the centrifugal rate and the centrifugal time, the metastable state silk fibroin nano-particle solution with homogeneous structure is formed finally. Meanwhile, the preparation method is simple in technology, and the obtained product has no residual organic solvent and is good in biocompatibility.

Description

technical field [0001] The invention relates to the technical field of nanoparticles, in particular to a metastable silk fibroin nanoparticle and a method for preparing a metastable silk fibroin nanoparticle solution. Background technique [0002] Due to its good biocompatibility, excellent mechanical properties, and adjustable degradation rate, silk fibroin can stabilize drug properties, prepare solution-based drugs, and regulate drug loading. It is a universal carrier for controlled drug release. According to the needs of different drug delivery applications, silk fibroin can be regenerated into various forms such as injectable nanoparticles, microspheres, hydrogels and adhesives, as well as fixed-position implanted films and scaffolds. Further modifications to silk fibroin were used to optimize the structure, composition, and crystallinity, so that the product could match targeted drugs and have a programmable release behavior while ensuring drug activity. Because silk f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/51A61K47/42C08J3/03C08L89/00
CPCA61K9/08A61K9/5169C08J3/03C08J2389/00
Inventor 吕强肖丽媖
Owner 苏州至明至信生物科技有限公司