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Application of chlorogenic acid in preparation of medicine for treating melanoma and medicine for treating melanoma

A technology of melanoma and chlorogenic acid, applied in the field of biomedicine and melanoma treatment

Inactive Publication Date: 2015-06-10
SICHUAN JIUZHANG BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Up to now, there is no relevant report on the treatment of melanoma with chlorogenic acid

Method used

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  • Application of chlorogenic acid in preparation of medicine for treating melanoma and medicine for treating melanoma
  • Application of chlorogenic acid in preparation of medicine for treating melanoma and medicine for treating melanoma
  • Application of chlorogenic acid in preparation of medicine for treating melanoma and medicine for treating melanoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Chlorogenic acid promotes the polarization of monocytes to M1 macrophages

[0038] (1) Establishment of animal models

[0039] The mouse melanoma B16 model is a C57BL / 6 mouse, male, 18-22g. During the experiment, take well-grown tumor tissue, cut it into pieces, grind it, filter it, and dilute it with sterile normal saline at a ratio of 1:3 to make a tumor cell suspension, and inoculate 0.2ml of the tumor cell suspension on the back of each mouse’s armpit . Animals were randomly grouped, weighed, and dosed started the day after inoculation. The administration volume of chlorogenic acid injection was intraperitoneally injecting 0.2ml per 10g of mice, once a day, for 13 consecutive days.

[0040] The experimental animals were divided into 4 groups, negative control group, chlorogenic acid 5 mg / kg, chlorogenic acid 10 mg / kg, and chlorogenic acid 20 mg / kg three dosage groups. 15 animals per group. The animals were sacrificed the next day after chlorogenic aci...

Embodiment 2

[0046] Example 2 Chlorogenic acid maintains M1 macrophages and inhibits the polarization of M1 to M2

[0047] The mouse melanoma B16 model is a C57BL / 6 mouse, male, 18-22g. During the experiment, take well-grown tumor tissue, cut it into pieces, grind it, filter it, and dilute it with sterile normal saline at a ratio of 1:3 to make a tumor cell suspension, and inoculate 0.2ml of the tumor cell suspension on the back of each mouse’s armpit . Animals were randomly grouped, weighed, and dosed started the day after inoculation. The administration volume of chlorogenic acid injection was intraperitoneally injecting 0.2ml per 10g of mice, once a day, for 13 consecutive days.

[0048] Experimental animals were divided into 4 groups, respectively: (1) control group (LPS+INFγ), (2) LPS and INFγ combined with chlorogenic acid 5 mg / kg group, (3) LPS and INFγ combined with chlorogenic acid 10 mg / kg kg combined drug group, (4) LPS and INFγ combined with chlorogenic acid 20 mg / kg group. ...

Embodiment 3

[0054] Example 3 Chlorogenic acid promotes the polarization of M2 macrophages into M1 macrophages

[0055] (1) Establishment of animal models

[0056] The mouse melanoma B16 model is a C57BL / 6 mouse, male, 18-22g. During the experiment, take well-grown tumor tissue, cut it into pieces, grind it, filter it, and dilute it with sterile normal saline at a ratio of 1:3 to make a tumor cell suspension, and inoculate 0.2ml of the tumor cell suspension on the back of each mouse’s armpit . Animals were randomly grouped, weighed, and dosed started the day after inoculation. The administration volume of chlorogenic acid injection was intraperitoneally injecting 0.2ml per 10g of mice, once a day, for 13 consecutive days.

[0057] Experimental animals were divided into 5 groups, respectively: (1) negative control group, (2) control group (IL-4), (3) IL-4 and chlorogenic acid 5mg / kg combined drug group, (3) IL-4 4 combined with chlorogenic acid 10 mg / kg group, (3) IL-4 combined with chl...

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Abstract

The invention discloses an application of a chlorogenic acid in preparation of a medicine for treating melanoma and the medicine for treating melanoma. An experiment proves that the chlorogenic acid is capable of promoting monocyte to polarize towards M1 macrophages, inhibiting the monocyte to polarize towards M2 macrophages, inhibiting the M1 macrophages to transform towards the M2 macrophages, inhibiting the M2 macrophages to transform towards the M1 macrophages, so that the target of treating the melanoma is finally reached.

Description

technical field [0001] The present invention relates to the field of biomedicine, especially the field of melanoma treatment, specifically the use of chlorogenic acid in the preparation of medicine for treating melanoma and the medicine for treating melanoma. The invention realizes the treatment of melanoma through the regulation of chlorogenic acid on macrophage polarization. Background technique [0002] Chlorogenic acid is a phenol isolated from the leaves and fruits of dicotyledonous plants (such as honeysuckle leaves, coffee beans, and sunflowers), which are produced by shikimic acid pathways during aerobic respiration in plants. A phenylpropanoid compound. It is an important component widely found in various plants, and plants related to daily life include sunflower seeds, fruits (apples, pears, grapes), vegetables (potatoes), soybeans, wheat, cocoa beans, coffee beans, sea buckthorn fruits Both traditional Chinese herbal medicines (Eulcommia ulmoides and honeysuckle...

Claims

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Application Information

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IPC IPC(8): A61K31/216A61K45/00A61P35/00A61K33/243
CPCA61K31/337A61K31/513A61K31/675A61K31/704A61P35/00A61P17/00A61K31/216A61K45/06A61K33/243A61K33/24A61K2300/00A61K9/0019A61K9/0053A61K31/05A61K31/191C07C69/732
Inventor 张洁陈晓光黄骏黄望
Owner SICHUAN JIUZHANG BIO TECH CO LTD
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