Application of chlorogenic acid in preparation of medicine for treating melanoma and medicine for treating melanoma

A technology of melanoma and chlorogenic acid, applied in the field of biomedicine and melanoma treatment

Inactive Publication Date: 2015-06-10
SICHUAN JIUZHANG BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Up to now, there is no relevant report on

Method used

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  • Application of chlorogenic acid in preparation of medicine for treating melanoma and medicine for treating melanoma
  • Application of chlorogenic acid in preparation of medicine for treating melanoma and medicine for treating melanoma
  • Application of chlorogenic acid in preparation of medicine for treating melanoma and medicine for treating melanoma

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0037] Example 1 Chlorogenic acid promotes the polarization of monocytes to M1 macrophages

[0038] (1) Establishment of animal model

[0039] Mouse melanoma B16 model, use C57BL / 6 mice, male, 18-22g. During the experiment, the well-growing tumor tissue was cut, ground, filtered, and diluted with sterile saline at a ratio of 1:3 to prepare a tumor cell suspension. Each mouse was inoculated with 0.2ml tumor cell suspension on the back of the axilla. . The animals were randomly divided into groups the next day after the inoculation, weighed, and the administration started. The administration volume of chlorogenic acid injection is 0.2ml per 10g mouse intraperitoneally, once a day for 13 consecutive days.

[0040] The experimental animals were divided into 4 groups, the negative control group, chlorogenic acid 5mg / kg, chlorogenic acid 10mg / kg, chlorogenic acid 20mg / kg three dose groups. 15 animals per group. The animals were sacrificed the next day after the chlorogenic acid was di...

Example Embodiment

[0046] Example 2 Chlorogenic acid maintains M1 macrophages and inhibits the polarization of M1 to M2

[0047] Mouse melanoma B16 model, use C57BL / 6 mice, male, 18-22g. During the experiment, the well-growing tumor tissue was cut, ground, filtered, and diluted with sterile saline at a ratio of 1:3 to prepare a tumor cell suspension. Each mouse was inoculated with 0.2ml tumor cell suspension on the back of the axilla. . The animals were randomly divided into groups the next day after the inoculation, weighed, and the administration started. The administration volume of chlorogenic acid injection is 0.2ml per 10g mouse intraperitoneally, once a day for 13 consecutive days.

[0048] The experimental animals were divided into 4 groups, namely: (1) control group (LPS+INFγ), (2) LPS and INFγ combined with chlorogenic acid 5 mg / kg group, (3) LPS and INFγ and chlorogenic acid 10 mg / kg kg combined medication group, (4) LPS and INFγ combined with chlorogenic acid 20mg / kg group. 15 animals...

Example Embodiment

[0054] Example 3 Chlorogenic acid promotes polarization of M2 type macrophages into M1 type macrophages

[0055] (1) Establishment of animal model

[0056] Mouse melanoma B16 model, use C57BL / 6 mice, male, 18-22g. During the experiment, the well-growing tumor tissue was cut, ground, filtered, and diluted with sterile saline at a ratio of 1:3 to prepare a tumor cell suspension. Each mouse was inoculated with 0.2ml tumor cell suspension on the back of the axilla. . The animals were randomly divided into groups the next day after the inoculation, weighed, and the administration started. The administration volume of chlorogenic acid injection is 0.2ml per 10g mouse intraperitoneally, once a day for 13 consecutive days.

[0057] The experimental animals were divided into 5 groups, namely: (1) negative control group, (2) control group (IL-4), (3) IL-4 and chlorogenic acid 5mg / kg combination group, (3) IL- 4 Combined medication group with chlorogenic acid 10mg / kg, (3) IL-4 combined with...

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Abstract

The invention discloses an application of a chlorogenic acid in preparation of a medicine for treating melanoma and the medicine for treating melanoma. An experiment proves that the chlorogenic acid is capable of promoting monocyte to polarize towards M1 macrophages, inhibiting the monocyte to polarize towards M2 macrophages, inhibiting the M1 macrophages to transform towards the M2 macrophages, inhibiting the M2 macrophages to transform towards the M1 macrophages, so that the target of treating the melanoma is finally reached.

Description

technical field [0001] The present invention relates to the field of biomedicine, especially the field of melanoma treatment, specifically the use of chlorogenic acid in the preparation of medicine for treating melanoma and the medicine for treating melanoma. The invention realizes the treatment of melanoma through the regulation of chlorogenic acid on macrophage polarization. Background technique [0002] Chlorogenic acid is a phenol isolated from the leaves and fruits of dicotyledonous plants (such as honeysuckle leaves, coffee beans, and sunflowers), which are produced by shikimic acid pathways during aerobic respiration in plants. A phenylpropanoid compound. It is an important component widely found in various plants, and plants related to daily life include sunflower seeds, fruits (apples, pears, grapes), vegetables (potatoes), soybeans, wheat, cocoa beans, coffee beans, sea buckthorn fruits Both traditional Chinese herbal medicines (Eulcommia ulmoides and honeysuckle...

Claims

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Application Information

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IPC IPC(8): A61K31/216A61K45/00A61P35/00A61K33/243
CPCA61K31/337A61K31/513A61K31/675A61K31/704A61P35/00A61P17/00A61K31/216A61K45/06A61K33/243A61K33/24A61K2300/00A61K9/0019A61K9/0053A61K31/05A61K31/191C07C69/732
Inventor 张洁陈晓光黄骏黄望
Owner SICHUAN JIUZHANG BIO TECH CO LTD
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