Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for measuring concentration of skullcapflavone II in plasma

A plasma and concentration technology, applied in measuring devices, instruments, scientific instruments, etc., to achieve the effects of high sensitivity, fast method and easy operation

Inactive Publication Date: 2016-10-12
JIANGSU PROVINCIAL HOSPITAL OF TCM
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There are currently no literature reports on the in vivo assay of Skullcapflavone-II

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for measuring concentration of skullcapflavone II in plasma
  • Method for measuring concentration of skullcapflavone II in plasma
  • Method for measuring concentration of skullcapflavone II in plasma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0029] Example: Determination of Skullcapflavone-II Concentration in Canine Plasma

[0030] 1. Experimental materials and instruments

[0031] skullcapflavone-II: provided by Zelang Pharmaceutical Group (Nanjing, China); new psoralen isoflavone (internal standard): purchased from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China); test water: ultrapure water; methanol : Chromatographically pure (Merck Company).

[0032] Agilent ZORBAX SB-C 18 Column (150mm×2.1mm I.D., 5μm, Agilent Technologies, Wilmington, DE, USA); API4000 LC-MS / MS triple quadrupole mass spectrometer (AB Co., Ltd., USA), chromatographic workstation Analyst 1.4.2; CPA225D electronic analysis Balance (Sartorius Co., Ltd., Germany); WH-2 Micro Vortex Mixer (Shanghai Huxi Analytical Instrument Factory); Milli-Q System Water Purifier (Micropore, Bedford, MA, USA); Biofuge PrimoR freezer High-speed centrifuge (German Heraeus company).

[0033] 2. Liquid conditions ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
lengthaaaaaaaaaa
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for measuring concentration of 5,2'-dyhydroxyl,6,7,8,5'-tetramethoxyflavone (skullcapflavone II) in plasma. A liquid chromatography-mass spectrometry system is used for carrying out measuring, a sample to be measured is firstly taken, a certain amount of organic solvent is added for medicine liquid-liquid extraction, after pretreatment, chromatographic column separation is carried out, and a mass spectrometry detector is used for carrying out detection. According to the method, quickness and accuracy are achieved, sensitivity is high, and operation is easy and convenient; the method is suitable for measuring the concentration of skullcapflavone II in the plasma.

Description

technical field [0001] The invention belongs to the technical field of biological detection, in particular to a method for measuring the concentration of 5,2'-dihydroxyl, 6,7,8,5'-tetramethoxyflavone (skullcapflavone II) in plasma. Background technique [0002] Skullcapflavone-II, namely 5,2'-dihydroxy, 6,7,8,5'-tetramethoxyflavone, is a new flavonoid compound isolated from the medicinal plant Scutellaria baicalensis. SKullcapflavone-II has anti-inflammatory, viral and immunomodulatory effects. In addition, many ingredients have the effects of antipruritic, regulating metabolic disorders, neuroprotection, promoting angiogenesis, anti-cancer and anti-HIV. Skullcapflavone-II has multiple biological properties such as putative role for cytotoxicity, effect on histamine release from mast cells, inhibition of plasminogen activator type 1 elevation inhibitor by trypsin and anti-asthmatic airway inflammatory response. [0003] Its structural formula is as follows: [0004] ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 刘史佳张农山戴国梁居文政储继红李长印许美娟吴婷张军冯丽雯
Owner JIANGSU PROVINCIAL HOSPITAL OF TCM
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com